Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Oct;124(1):290–295. doi: 10.1128/jb.124.1.290-295.1975

"Killer" character does not influence the transmission of mitochondrial genes in Saccharomyces cerevisiae.

R A Young, P S Perlman
PMCID: PMC235895  PMID: 1100604

Abstract

Three cytoplasmic genetic elements have been shown to be separate from mitochondrial deoxyribonucleic acid (DNA), [rho], in Saccharomyces cerevisiae: the killer character [k], omicron-DNA, and psi [psi]. Griffiths has suggested genetic interactions between [VENR] and [TETR] mutants possibly located on omicron-DNA and mitochondrial genetic markers, but possible interactions between the best characterized of the three, the killer character, and mitochondrial DNA have not been investigated. To test this we isolated cycloheximide-induced nonkiller segregants (NKS) of killer cells with suitable genetic markers and mated them in [k] x [k], [k] x [k], and (NKS) x (NKS) combinations. No differences in quantitative mitochondrial marker transmission between these groups were found in crosses illustrating the mitochondrial phenomena of bias, polarity, and suppressiveness. Our studies show that no intercellular interactions between [k] and (NKS) cells influence mitochondrial transmission genetics. Intracellular interactions between the smaller double-stranded ribonucleic acid of [k] and mitochondrial DNA also were not detected.

Full text

PDF
290

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry E. A., Bevan E. A. A new species of double-stranded RNA from yeast. Nature. 1972 Sep 29;239(5370):279–280. doi: 10.1038/239279a0. [DOI] [PubMed] [Google Scholar]
  2. Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
  3. Bevan E. A., Somers J. M. Somatic segregation of the killer (k) and neutral (n) cytoplasmic genetic determinants in yeast. Genet Res. 1969 Aug;14(1):71–77. doi: 10.1017/s0016672300001865. [DOI] [PubMed] [Google Scholar]
  4. Callen D. The effect of mating type on the polarity of mitochondrial gene transmission in Saccharomyces cerevisiae. Mol Gen Genet. 1974;128(4):321–329. doi: 10.1007/BF00268519. [DOI] [PubMed] [Google Scholar]
  5. Clark-Walker G. D. Size distribution of circular DNA from petite-mutant yeast lacking rho DNA. Eur J Biochem. 1973 Jan 15;32(2):263–267. doi: 10.1111/j.1432-1033.1973.tb02606.x. [DOI] [PubMed] [Google Scholar]
  6. Coen D., Deutsch J., Netter P., Petrochilo E., Slonimski P. P. Mitochondrial genetics. I. Methodology and phenomenology. Symp Soc Exp Biol. 1970;24:449–496. [PubMed] [Google Scholar]
  7. Dujon B., Slonimski P. P., Weill L. Mitochondrial genetics IX: A model for recombination and segregation of mitochondrial genomes in saccharomyces cerevisiae. Genetics. 1974 Sep;78(1):415–437. doi: 10.1093/genetics/78.1.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EPHRUSS B., GRANDCHAMP S. ETUDES SUR LA SUPPRESSIVIT'E DES MUTANTS 'A DEFICIENCE RESPIRATOIRE DE LA LEVURE. I. EXISTENCE AU NIVEAU CELLULAIRE DE DIVERS "DEGR'ES DE SUPPRESSIVIT'E". Heredity (Edinb) 1965 Feb;20:1–7. doi: 10.1038/hdy.1965.1. [DOI] [PubMed] [Google Scholar]
  9. Ephrussi B., Jakob H., Grandchamp S. Etudes Sur La SuppressivitE Des Mutants a Deficience Respiratoire De La Levure. II. Etapes De La Mutation Grande En Petite Provoquee Par Le Facteur Suppressif. Genetics. 1966 Jul;54(1):1–29. doi: 10.1093/genetics/54.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ephrussi B., de Margerie-Hottinguer H., Roman H. SUPPRESSIVENESS: A NEW FACTOR IN THE GENETIC DETERMINISM OF THE SYNTHESIS OF RESPIRATORY ENZYMES IN YEAST. Proc Natl Acad Sci U S A. 1955 Dec 15;41(12):1065–1071. doi: 10.1073/pnas.41.12.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fink G. R., Styles C. A. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. doi: 10.1073/pnas.69.10.2846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gillham N. W. Genetic analysis of the chloroplast and mitochondrial genomes. Annu Rev Genet. 1974;8:347–391. doi: 10.1146/annurev.ge.08.120174.002023. [DOI] [PubMed] [Google Scholar]
  13. Goldthwaite C. D., Cryer D. R., Marmur J. Effect of carbon source on the replication and transmission of yeast mitochondrial genomes. Mol Gen Genet. 1974;133(2):87–104. doi: 10.1007/BF00264830. [DOI] [PubMed] [Google Scholar]
  14. Guerineau M., Grandchamp C., Paoletti C., Slonimski P. Characterization of a new class of circular DNA molecules in yeast. Biochem Biophys Res Commun. 1971 Feb 5;42(3):550–557. doi: 10.1016/0006-291x(71)90406-2. [DOI] [PubMed] [Google Scholar]
  15. Howell N., Hall R. M., Linnane A. W., Lukins H. B. Genetic analyses of the polarity alleles in recombinants from mitochondrial genetic crosses. J Bacteriol. 1974 Sep;119(3):1063–1065. doi: 10.1128/jb.119.3.1063-1065.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lancashire, Griffiths D. E. Studies on energy-linked reactions: genetic analysis of venturicidin-resistant mutants. Eur J Biochem. 1975 Feb 21;51(2):403–413. doi: 10.1111/j.1432-1033.1975.tb03940.x. [DOI] [PubMed] [Google Scholar]
  17. Lancashire W. E., Griffiths D. E. Studies on energy-linked reactions: isolation, characterisation and genetic analysis of trialkyl-tin-resistant mutants of Saccharomyces cerevisiae. Eur J Biochem. 1975 Feb 21;51(2):377–392. doi: 10.1111/j.1432-1033.1975.tb03938.x. [DOI] [PubMed] [Google Scholar]
  18. OGUR M., ST. JOHN R., NAGAI S. Tetrazolium overlay technique for population studies of respiration deficiency in yeast. Science. 1957 May 10;125(3254):928–929. doi: 10.1126/science.125.3254.928. [DOI] [PubMed] [Google Scholar]
  19. Perlman P. S., Birky C. W., Jr Mitochondrial genetics in Bakers' yeast: a molecular mechanism for recombinational polarity and suppressiveness. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4612–4616. doi: 10.1073/pnas.71.11.4612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SHERMAN F., EPHRUSSI B. The relationship between respiratory deficiency and suppressiveness in yeast as determined with segregational mutants. Genetics. 1962 Jun;47:695–700. doi: 10.1093/genetics/47.6.695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Somers J. M., Bevan E. A. The inheritance of the killer character in yeast. Genet Res. 1969 Feb;13(1):71–83. doi: 10.1017/s0016672300002743. [DOI] [PubMed] [Google Scholar]
  22. Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wolf K., Dujon B., Slonimski P. P. Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae. Mol Gen Genet. 1973 Sep 5;125(1):53–90. doi: 10.1007/BF00292983. [DOI] [PubMed] [Google Scholar]
  24. Woods D. R., Bevan E. A. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol. 1968 Apr;51(1):115–126. doi: 10.1099/00221287-51-1-115. [DOI] [PubMed] [Google Scholar]
  25. Young C. S., Cox B. S. Extrachromosomal elements in a super-suppression system of yeast. II. Relations with other extrachromosomal elements. Heredity (Edinb) 1972 Apr;28(2):189–199. doi: 10.1038/hdy.1972.24. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES