Abstract
Extracts of recessive UGA suppressor strains, designated supK, are deficient in transfer ribonucleic acid (tRNA)-methylating activity when compared to wild-type extracts. Moreover, the tRNA from suppressor strains is methyl deficient when compared to wild-type tRNA. This deficiency is due to the lack of a single tRNA methylase activity in suppressor strains. UGA suppressor activity may be caused by the miscoding of one or more methyl-deficient tRNA's in supK strains.
Full text
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Selected References
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- Atkins J. F., Ryce S. UGA and non-triplet suppressor reading of the genetic code. Nature. 1974 Jun 7;249(457):527–530. doi: 10.1038/249527a0. [DOI] [PubMed] [Google Scholar]
- Björk G. R., Isaksson L. A. Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA. J Mol Biol. 1970 Jul 14;51(1):83–100. doi: 10.1016/0022-2836(70)90272-x. [DOI] [PubMed] [Google Scholar]
- Chater K. F. Dominance of the wild-type alleles of methionine regulatory genes in Salmonella typhimurium. J Gen Microbiol. 1970 Sep;63(1):95–109. doi: 10.1099/00221287-63-1-95. [DOI] [PubMed] [Google Scholar]
- Cortese R., Kammen H. O., Spengler S. J., Ames B. N. Biosynthesis of pseudouridine in transfer ribonucleic acid. J Biol Chem. 1974 Feb 25;249(4):1103–1108. [PubMed] [Google Scholar]
- Dalal F. R., Gots R. E., Gots J. S. Mechanism of adenine inhibition in adenine-sensitive mutants of Salmonella typhimurium. J Bacteriol. 1966 Feb;91(2):507–513. doi: 10.1128/jb.91.2.507-513.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gold M., Hurwitz J., Anders M. THE ENZYMATIC METHYLATION OF RNA AND DNA, II. ON THE SPECIES SPECIFICITY OF THE METHYLATION ENZYMES. Proc Natl Acad Sci U S A. 1963 Jul;50(1):164–169. doi: 10.1073/pnas.50.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodman H. M., Abelson J., Landy A., Brenner S., Smith J. D. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA. Nature. 1968 Mar 16;217(5133):1019–1024. doi: 10.1038/2171019a0. [DOI] [PubMed] [Google Scholar]
- Greene R. C., Su C. H., Holloway C. T. S-Adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1120–1126. doi: 10.1016/0006-291x(70)90355-4. [DOI] [PubMed] [Google Scholar]
- HOSKINSON R. M., KHORANA H. G. STUDIES ON POLYNUCLEOTIDES. XLI. PURIFICATION OF PHENYLALANINE-SPECIFIC TRANSFER RIBONUCLEIC ACID FROM YEAST BY COUNTERCURRENT DISTRIBUTION. J Biol Chem. 1965 May;240:2129–2134. [PubMed] [Google Scholar]
- HURWITZ J., GOLD M., ANDERS M. THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. 3. PURIFICATION OF SOLUBLE RIBONUCLEIC ACID-METHYLATING ENZYMES. J Biol Chem. 1964 Oct;239:3462–3473. [PubMed] [Google Scholar]
- Hirsh D., Gold L. Translation of the UGA triplet in vitro by tryptophan transfer RNA's. J Mol Biol. 1971 Jun 14;58(2):459–468. doi: 10.1016/0022-2836(71)90363-9. [DOI] [PubMed] [Google Scholar]
- Hirsh D. Tryptophan transfer RNA as the UGA suppressor. J Mol Biol. 1971 Jun 14;58(2):439–458. doi: 10.1016/0022-2836(71)90362-7. [DOI] [PubMed] [Google Scholar]
- Hobson A. C., Smith D. A. S-adenosylmethionine synthetase in methionine regulatory mutants of Salmonella typhimurium. Mol Gen Genet. 1973 Oct 16;126(1):7–18. doi: 10.1007/BF00333477. [DOI] [PubMed] [Google Scholar]
- Hoffman E. P., Wilhelm R. C. Genetic mapping and dominance of the amber suppressor, Su1 (supD), in Escherichia coli K-12. J Bacteriol. 1970 Jul;103(1):32–36. doi: 10.1128/jb.103.1.32-36.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawrence D. A., Smith D. A., Rowbury R. J. Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics. 1968 Apr;58(4):473–492. doi: 10.1093/genetics/58.4.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marinus M. G., Morris N. R., Söll D., Kwong T. C. Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. J Bacteriol. 1975 Apr;122(1):257–265. doi: 10.1128/jb.122.1.257-265.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NISHIMURA S., NOVELLI G. D. AMINO ACID ACCEPTOR ACTIVITY OF ENZYMICALLY ALTERED SOLUBLE RNA FROM ESCHERICHIA COLI. Biochim Biophys Acta. 1964 Apr 27;80:574–586. doi: 10.1016/0926-6550(64)90302-0. [DOI] [PubMed] [Google Scholar]
- Phillips J. H., Kjellin-Stråby K. Studies on microbial ribonucleic acid. IV. Two mutants of Saccharomyces cerevisiae lacking N-2-dimethylguanine in soluble ribonucleic acid. J Mol Biol. 1967 Jun 28;26(3):509–518. doi: 10.1016/0022-2836(67)90318-x. [DOI] [PubMed] [Google Scholar]
- Reeves R. H., Imura N., Schwam H., Weiss G. B., Schulman L. H., Chambers R. W. Transfer RNA, I. Isolation and characterization of a new yeast alanine transfer RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1450–1457. doi: 10.1073/pnas.60.4.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reeves R. H., Roth J. R. A recessive UGA suppressor. J Mol Biol. 1971 Mar 28;56(3):523–533. doi: 10.1016/0022-2836(71)90399-8. [DOI] [PubMed] [Google Scholar]
- Riddle D. L., Roth J. R. Frameshift suppressors. 3. Effects of suppressor mutations on transfer RNA. J Mol Biol. 1972 May 28;66(3):495–506. doi: 10.1016/0022-2836(72)90429-9. [DOI] [PubMed] [Google Scholar]
- Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
- Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
- Shugart L., Chastain B. H., Novelli G. D., Stulberg M. P. Restoration of aminoacylation activity of undermethylated transfer RNA by in vitro methylation. Biochem Biophys Res Commun. 1968 May 10;31(3):404–409. doi: 10.1016/0006-291x(68)90490-7. [DOI] [PubMed] [Google Scholar]
- Signer E. R., Beckwith J. R., Brenner S. Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965 Nov;14(1):153–166. doi: 10.1016/s0022-2836(65)80237-6. [DOI] [PubMed] [Google Scholar]
- Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]
- Soll L. Mutational alterations of tryptophan-specific transfer RNA that generate translation suppressors of the UAA, UAG and UGA nonsense codons. J Mol Biol. 1974 Jun 25;86(2):233–243. doi: 10.1016/0022-2836(74)90015-1. [DOI] [PubMed] [Google Scholar]
- Srinivasan P. R., Borek E. THE SPECIES VARIATION OF RNA METHYLASE. Proc Natl Acad Sci U S A. 1963 Apr;49(4):529–533. doi: 10.1073/pnas.49.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Starr J. L., Sells B. H. Methylated ribonucleic acids. Physiol Rev. 1969 Jul;49(3):623–669. doi: 10.1152/physrev.1969.49.3.623. [DOI] [PubMed] [Google Scholar]
- Svensson I., Isaksson L., Henningsson A. Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidine. Biochim Biophys Acta. 1971 May 13;238(2):331–337. doi: 10.1016/0005-2787(71)90100-6. [DOI] [PubMed] [Google Scholar]
- VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
- Yaniv M., Folk W. R., Berg P., Soll L. A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG. J Mol Biol. 1974 Jun 25;86(2):245–260. doi: 10.1016/0022-2836(74)90016-3. [DOI] [PubMed] [Google Scholar]