Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Oct;124(1):332–340. doi: 10.1128/jb.124.1.332-340.1975

Transfer ribonucleic acid methylase deficiency found in UGA supressor strains.

R H Reeves, J R Roth
PMCID: PMC235900  PMID: 1100607

Abstract

Extracts of recessive UGA suppressor strains, designated supK, are deficient in transfer ribonucleic acid (tRNA)-methylating activity when compared to wild-type extracts. Moreover, the tRNA from suppressor strains is methyl deficient when compared to wild-type tRNA. This deficiency is due to the lack of a single tRNA methylase activity in suppressor strains. UGA suppressor activity may be caused by the miscoding of one or more methyl-deficient tRNA's in supK strains.

Full text

PDF
332

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins J. F., Ryce S. UGA and non-triplet suppressor reading of the genetic code. Nature. 1974 Jun 7;249(457):527–530. doi: 10.1038/249527a0. [DOI] [PubMed] [Google Scholar]
  2. Björk G. R., Isaksson L. A. Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA. J Mol Biol. 1970 Jul 14;51(1):83–100. doi: 10.1016/0022-2836(70)90272-x. [DOI] [PubMed] [Google Scholar]
  3. Chater K. F. Dominance of the wild-type alleles of methionine regulatory genes in Salmonella typhimurium. J Gen Microbiol. 1970 Sep;63(1):95–109. doi: 10.1099/00221287-63-1-95. [DOI] [PubMed] [Google Scholar]
  4. Cortese R., Kammen H. O., Spengler S. J., Ames B. N. Biosynthesis of pseudouridine in transfer ribonucleic acid. J Biol Chem. 1974 Feb 25;249(4):1103–1108. [PubMed] [Google Scholar]
  5. Dalal F. R., Gots R. E., Gots J. S. Mechanism of adenine inhibition in adenine-sensitive mutants of Salmonella typhimurium. J Bacteriol. 1966 Feb;91(2):507–513. doi: 10.1128/jb.91.2.507-513.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gold M., Hurwitz J., Anders M. THE ENZYMATIC METHYLATION OF RNA AND DNA, II. ON THE SPECIES SPECIFICITY OF THE METHYLATION ENZYMES. Proc Natl Acad Sci U S A. 1963 Jul;50(1):164–169. doi: 10.1073/pnas.50.1.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goodman H. M., Abelson J., Landy A., Brenner S., Smith J. D. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA. Nature. 1968 Mar 16;217(5133):1019–1024. doi: 10.1038/2171019a0. [DOI] [PubMed] [Google Scholar]
  8. Greene R. C., Su C. H., Holloway C. T. S-Adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis. Biochem Biophys Res Commun. 1970 Mar 27;38(6):1120–1126. doi: 10.1016/0006-291x(70)90355-4. [DOI] [PubMed] [Google Scholar]
  9. HOSKINSON R. M., KHORANA H. G. STUDIES ON POLYNUCLEOTIDES. XLI. PURIFICATION OF PHENYLALANINE-SPECIFIC TRANSFER RIBONUCLEIC ACID FROM YEAST BY COUNTERCURRENT DISTRIBUTION. J Biol Chem. 1965 May;240:2129–2134. [PubMed] [Google Scholar]
  10. HURWITZ J., GOLD M., ANDERS M. THE ENZYMATIC METHYLATION OF RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID. 3. PURIFICATION OF SOLUBLE RIBONUCLEIC ACID-METHYLATING ENZYMES. J Biol Chem. 1964 Oct;239:3462–3473. [PubMed] [Google Scholar]
  11. Hirsh D., Gold L. Translation of the UGA triplet in vitro by tryptophan transfer RNA's. J Mol Biol. 1971 Jun 14;58(2):459–468. doi: 10.1016/0022-2836(71)90363-9. [DOI] [PubMed] [Google Scholar]
  12. Hirsh D. Tryptophan transfer RNA as the UGA suppressor. J Mol Biol. 1971 Jun 14;58(2):439–458. doi: 10.1016/0022-2836(71)90362-7. [DOI] [PubMed] [Google Scholar]
  13. Hobson A. C., Smith D. A. S-adenosylmethionine synthetase in methionine regulatory mutants of Salmonella typhimurium. Mol Gen Genet. 1973 Oct 16;126(1):7–18. doi: 10.1007/BF00333477. [DOI] [PubMed] [Google Scholar]
  14. Hoffman E. P., Wilhelm R. C. Genetic mapping and dominance of the amber suppressor, Su1 (supD), in Escherichia coli K-12. J Bacteriol. 1970 Jul;103(1):32–36. doi: 10.1128/jb.103.1.32-36.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lawrence D. A., Smith D. A., Rowbury R. J. Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics. 1968 Apr;58(4):473–492. doi: 10.1093/genetics/58.4.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marinus M. G., Morris N. R., Söll D., Kwong T. C. Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. J Bacteriol. 1975 Apr;122(1):257–265. doi: 10.1128/jb.122.1.257-265.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NISHIMURA S., NOVELLI G. D. AMINO ACID ACCEPTOR ACTIVITY OF ENZYMICALLY ALTERED SOLUBLE RNA FROM ESCHERICHIA COLI. Biochim Biophys Acta. 1964 Apr 27;80:574–586. doi: 10.1016/0926-6550(64)90302-0. [DOI] [PubMed] [Google Scholar]
  18. Phillips J. H., Kjellin-Stråby K. Studies on microbial ribonucleic acid. IV. Two mutants of Saccharomyces cerevisiae lacking N-2-dimethylguanine in soluble ribonucleic acid. J Mol Biol. 1967 Jun 28;26(3):509–518. doi: 10.1016/0022-2836(67)90318-x. [DOI] [PubMed] [Google Scholar]
  19. Reeves R. H., Imura N., Schwam H., Weiss G. B., Schulman L. H., Chambers R. W. Transfer RNA, I. Isolation and characterization of a new yeast alanine transfer RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1450–1457. doi: 10.1073/pnas.60.4.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reeves R. H., Roth J. R. A recessive UGA suppressor. J Mol Biol. 1971 Mar 28;56(3):523–533. doi: 10.1016/0022-2836(71)90399-8. [DOI] [PubMed] [Google Scholar]
  21. Riddle D. L., Roth J. R. Frameshift suppressors. 3. Effects of suppressor mutations on transfer RNA. J Mol Biol. 1972 May 28;66(3):495–506. doi: 10.1016/0022-2836(72)90429-9. [DOI] [PubMed] [Google Scholar]
  22. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  23. Russell R. L., Abelson J. N., Landy A., Gefter M. L., Brenner S., Smith J. D. Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol. 1970 Jan 14;47(1):1–13. doi: 10.1016/0022-2836(70)90397-9. [DOI] [PubMed] [Google Scholar]
  24. Shugart L., Chastain B. H., Novelli G. D., Stulberg M. P. Restoration of aminoacylation activity of undermethylated transfer RNA by in vitro methylation. Biochem Biophys Res Commun. 1968 May 10;31(3):404–409. doi: 10.1016/0006-291x(68)90490-7. [DOI] [PubMed] [Google Scholar]
  25. Signer E. R., Beckwith J. R., Brenner S. Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965 Nov;14(1):153–166. doi: 10.1016/s0022-2836(65)80237-6. [DOI] [PubMed] [Google Scholar]
  26. Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]
  27. Soll L. Mutational alterations of tryptophan-specific transfer RNA that generate translation suppressors of the UAA, UAG and UGA nonsense codons. J Mol Biol. 1974 Jun 25;86(2):233–243. doi: 10.1016/0022-2836(74)90015-1. [DOI] [PubMed] [Google Scholar]
  28. Srinivasan P. R., Borek E. THE SPECIES VARIATION OF RNA METHYLASE. Proc Natl Acad Sci U S A. 1963 Apr;49(4):529–533. doi: 10.1073/pnas.49.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Starr J. L., Sells B. H. Methylated ribonucleic acids. Physiol Rev. 1969 Jul;49(3):623–669. doi: 10.1152/physrev.1969.49.3.623. [DOI] [PubMed] [Google Scholar]
  30. Svensson I., Isaksson L., Henningsson A. Aminoacylation and polypeptide synthesis with tRNA lacking ribothymidine. Biochim Biophys Acta. 1971 May 13;238(2):331–337. doi: 10.1016/0005-2787(71)90100-6. [DOI] [PubMed] [Google Scholar]
  31. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  32. Yaniv M., Folk W. R., Berg P., Soll L. A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG. J Mol Biol. 1974 Jun 25;86(2):245–260. doi: 10.1016/0022-2836(74)90016-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES