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. 1975 Nov;124(2):661–667. doi: 10.1128/jb.124.2.661-667.1975

Chromosome-plasmid interaction in Escherichia coli K-12 carrying a thermosensitive plasmid, Rts1, in autonomous and in integrated states.

H Yoshimoto, M Yoshikawa
PMCID: PMC235952  PMID: 1102527

Abstract

An Hfr strain of Escherichia coli K-12 was obtained by integrative suppression with a thermosensitive plasmid, Rts1. The R plasmid was integrated into the chromosome between rif and thr, and transfer of the chromosome occurred counterclockwise. The thermosensitivity of host cell growth due to the dnaA mutation was markedly but not completely reduced in this integratively suppressed Hfr strain. When the dnaA mutation was removed by transducing the dnaA+ genome to this Hfr, the thermosensitivity of cell growth due to existence of Rts1 was suppressed in contrast to strains carrying it autonomously. Thermosensitivity of cell growth appeared again when the plasmid was detached from the chromosome to exist autonomously. Contrary to the effect on cell growth, the transfer of the chromosome and the plasmid itself and the ability to "restrict" T-even phages were still thermosensitive in all of these strains carrying Rts1, irrespective of its state of existence. The detached plasmid as well as the original Rts1 were segregated upon growth at 42 C. These data are discussed in relation to chromosome-plasmid interaction. One of the most important conculusions is that some plasmid genes, related to their replication, are phenotypically suppressed by the chromosome when it is integrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DiJoseph C. G., Bayer M. E., Kaji A. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts1. J Bacteriol. 1973 Jul;115(1):399–410. doi: 10.1128/jb.115.1.399-410.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DiJoseph C. G. The thermosensitive lesion in the replication of the drug resistance factor, Rts1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2515–2519. doi: 10.1073/pnas.71.6.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ikeda H., Tomizawa J. I. Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments. J Mol Biol. 1965 Nov;14(1):85–109. doi: 10.1016/s0022-2836(65)80232-7. [DOI] [PubMed] [Google Scholar]
  5. Ippen K., Shapiro J. A., Beckwith J. R. Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of lambda-lac transducing bacteriophages. J Bacteriol. 1971 Oct;108(1):5–9. doi: 10.1128/jb.108.1.5-9.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. Sotomura M., Yoshikawa M. Reinitiation of chromosome replication in the presence of chloramphenicol under an integratively suppressed state by R6K. J Bacteriol. 1975 May;122(2):623–628. doi: 10.1128/jb.122.2.623-628.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Terawaki Y., Kakizawa Y., Takayasu H., Yoshikawa M. Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R(KM) factor. Nature. 1968 Jul 20;219(5151):284–285. doi: 10.1038/219284a0. [DOI] [PubMed] [Google Scholar]
  10. Terawaki Y., Kishi H., Nakaya R. Integration of R plasmid Rts1 to the gal region of the Escherichia coli chromosome. J Bacteriol. 1975 Mar;121(3):857–862. doi: 10.1128/jb.121.3.857-862.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Terawaki Y., Takayasu H., Akiba T. Thermosensitive replication of a kanamycin resistance factor. J Bacteriol. 1967 Sep;94(3):687–690. doi: 10.1128/jb.94.3.687-690.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yokota T., Kanamaru Y., Mori R., Akiba T. Recombination between a thermosensitive kanamycin resistance factor and a nonthermosensitive multiple-drug resistant factor. J Bacteriol. 1969 Jun;98(3):863–873. doi: 10.1128/jb.98.3.863-873.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yoshikawa M. Identification and mapping of the replication genes of an R factor, R100-1, integrated into the chromosome of Escherichia coli K-12. J Bacteriol. 1974 Jun;118(3):1123–1131. doi: 10.1128/jb.118.3.1123-1131.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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