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. 1975 Nov;124(2):686–692. doi: 10.1128/jb.124.2.686-692.1975

Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae.

M Singh, W B Dempsey, P A Srere
PMCID: PMC235955  PMID: 1102528

Abstract

A pantothenate-requiring mutant of Klebsiella pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase (3.4 mol/mol of enzyme). Analysis of a double-labeled enzyme ([14C]pantothenate and [3H]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3H and 14C were associated solely with the smallest subunit, the acyl carrier protein of citrate lyase.

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Selected References

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