Abstract
Neurospora crassa ribosomes contain a species of ribonucleic acid (RNA) of molecular weight 54,000, similar to 5.8S ribosomal RNA previously described for other eukaryotic organisms. The 5.8S RNA from N. crassa was found to be released by heat treatment at 60 C from 25S ribosomal RNA but not from 18S ribosomal RNA. The base composition of N. crassa 5.8S RNA was similar to that of 5.8S RNA from Saccharomyces cerevisiae, but differed from animal 5.8S RNA. During the course of this study, it was discovered that N. crassa 25S ribosomal RNA had a number of internal cleavages that may exist in vivo.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Atchison B. A., Bourque D. P., Wildman S. G. Preservation of 23-S chloroplast RNA as a single chain of nucleotides. Biochim Biophys Acta. 1973 Dec 21;331(3):382–389. doi: 10.1016/0005-2787(73)90024-5. [DOI] [PubMed] [Google Scholar]
- INGLE J., KEY J. L., HOLM R. E. DEMONSTRATION AND CHARACTERIZATION OF A DNA-LIKE RNA IN EXCISED PLANT TISSUE. J Mol Biol. 1965 Apr;11:730–746. doi: 10.1016/s0022-2836(65)80031-6. [DOI] [PubMed] [Google Scholar]
- Ishikawa H. Primary and secondary nicks in the ribosomal ribonucleic acid of insects. Biochem Biophys Res Commun. 1973 Sep 5;54(1):301–307. doi: 10.1016/0006-291x(73)90923-6. [DOI] [PubMed] [Google Scholar]
- King H. W., Gould H. Low molecular weight ribonucleic acid in rabbit reticulocyte ribosomes. J Mol Biol. 1970 Aug;51(3):687–702. doi: 10.1016/0022-2836(70)90017-3. [DOI] [PubMed] [Google Scholar]
- Kochert G., Sansing N. Isolation and characterization of nucleic acids from Volvox carteri. Biochim Biophys Acta. 1971 May 27;238(3):397–405. doi: 10.1016/0005-2787(71)90614-9. [DOI] [PubMed] [Google Scholar]
- Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marrs B., Kaplan S. 23 s precursor ribosomal RNA of Rhodopseudomonas spheroides. J Mol Biol. 1970 Apr 28;49(2):297–317. doi: 10.1016/0022-2836(70)90247-0. [DOI] [PubMed] [Google Scholar]
- Payne P. I., Dyer T. A. Phytochrome and temperature relations in Lactuca sativa L. Grand Rapids seed germination after thermo-dormancy. Nat New Biol. 1972 Feb 2;235(57):145–147. [PubMed] [Google Scholar]
- Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
- Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
- Solymosy F., Fedorcsák I., Gulyás A., Farkas G. L., Ehrenberg L. A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. Eur J Biochem. 1968 Sep 24;5(4):520–527. doi: 10.1111/j.1432-1033.1968.tb00401.x. [DOI] [PubMed] [Google Scholar]
- Stanley W. M., Jr, Bock R. M. Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli. Biochemistry. 1965 Jul;4(7):1302–1311. doi: 10.1021/bi00883a014. [DOI] [PubMed] [Google Scholar]
- Stevens A. R., Pachler P. F. Discontinuity of 26 s rRNA in Acanthamoeba castellani. J Mol Biol. 1972 May 14;66(2):225–237. doi: 10.1016/0022-2836(72)90475-5. [DOI] [PubMed] [Google Scholar]
- Sy J., McCarty K. S. Characterization of 5.8-S RNA from a complex with 26-S ribosomal RNA from Arbacia punctulata. Biochim Biophys Acta. 1970 Jan 21;199(1):86–94. doi: 10.1016/0005-2787(70)90697-0. [DOI] [PubMed] [Google Scholar]
- Weinberg R. A., Penman S. Small molecular weight monodisperse nuclear RNA. J Mol Biol. 1968 Dec;38(3):289–304. doi: 10.1016/0022-2836(68)90387-2. [DOI] [PubMed] [Google Scholar]