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. 1975 Nov;124(2):784–790. doi: 10.1128/jb.124.2.784-790.1975

Transmissible toxin (hemolysin) plasmid in Streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid.

G M Dunny, D B Clewell
PMCID: PMC235968  PMID: 810482

Abstract

Streptococcus faecalis strain DS-5 has been shown previously to contain three plasmids, pAMalpha1, pAMbeta1, and pAMgamma1. Mixed incubation of DS-5 with strain JH2-2, a plasmid-free S. faecalis recipient, results in the transfer of pAMalpha1 (which determines resistance to tetracycline) and/or pAMgamma1. Analyses of recipients carrying various combinations of these plasmids have revealed the pAMgamma1 codes for toxin (hemolysin) production and two bacteriocin activities. JH2-2 strains carrying both pAMalpha1 and pAMgamma1, or pAMgamma1 only, can donate their plasmids to other recipients, whereas strains carrying only pAMalpha1 cannot serve as donors. This indicates that pAMgamma1 actually mobilizes the otherwise nontransferable pAMalpha1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Lewis M. J. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature. 1965 Nov 27;208(5013):843–849. doi: 10.1038/208843a0. [DOI] [PubMed] [Google Scholar]
  2. BROCK T. D., DAVIE J. M. PROBABLE IDENTITY OF A GROUP D HEMOLYSIN WITH A BACTERIOCINE. J Bacteriol. 1963 Oct;86:708–712. doi: 10.1128/jb.86.4.708-712.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bak A. L., Christiansen C., Stenderup A. Bacterial genome sizes determined by DNA renaturation studies. J Gen Microbiol. 1970 Dec;64(3):377–380. doi: 10.1099/00221287-64-3-377. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Franke A. E. Characterization of a plasmid determining resistance to erythromycin, lincomycin, and vernamycin Balpha in a strain Streptococcus pyogenes. Antimicrob Agents Chemother. 1974 May;5(5):534–537. doi: 10.1128/aac.5.5.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  6. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clewell D. B., Yagi Y., Bauer B. Plasmid-determined tetracycline resistance in Streptococcus faecalis: evidence for gene amplification during growth in presence of tetracycline. Proc Natl Acad Sci U S A. 1975 May;72(5):1720–1724. doi: 10.1073/pnas.72.5.1720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clewell D. B., Yagi Y., Dunny G. M., Schultz S. K. Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance. J Bacteriol. 1974 Jan;117(1):283–289. doi: 10.1128/jb.117.1.283-289.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cords B. R., McKay L. L., Guerry P. Extrachromosomal elements in group N streptococci. J Bacteriol. 1974 Mar;117(3):1149–1152. doi: 10.1128/jb.117.3.1149-1152.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Courvalin P. M., Carlier C., Chabbert Y. A. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus". Ann Inst Pasteur (Paris) 1972 Dec;123(6):755–759. [PubMed] [Google Scholar]
  11. Dunny G. M., Birch N., Hascall G., Clewell D. B. Isolation and characterization of plasmid deoxyribonucleic acid from Streptococcus mutans. J Bacteriol. 1973 Jun;114(3):1362–1364. doi: 10.1128/jb.114.3.1362-1364.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Granato P. A., Jackson R. W. Characterization of the A component of Streptococcus zymogenes lysin. J Bacteriol. 1971 Aug;107(2):551–556. doi: 10.1128/jb.107.2.551-556.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jacob A. E., Douglas G. J., Hobbs S. J. Self-transferable plasmids determining the hemolysin and bacteriocin of Streptococcus faecalis var. zymogenes. J Bacteriol. 1975 Mar;121(3):863–872. doi: 10.1128/jb.121.3.863-872.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Milliken C. E., Clowes R. C. Molecular structure of an R factor, its component drug-resistance determinants and transfer factor. J Bacteriol. 1973 Feb;113(2):1026–1033. doi: 10.1128/jb.113.2.1026-1033.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sherman J. M. The Enterococci and Related Streptococci. J Bacteriol. 1938 Feb;35(2):81–93. doi: 10.1128/jb.35.2.81-93.1938. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tomura T., Hirano T., Ito T., Yoshioka M. Transmission of bacteriocinogenicity by conjugation in group D streptococci. Jpn J Microbiol. 1973 Nov;17(6):445–452. doi: 10.1111/j.1348-0421.1973.tb00930.x. [DOI] [PubMed] [Google Scholar]
  18. Yag Y., Franke A. E., Clewell D. B. Plasmid-determined resistance to erythromycin: comparison of strains of streptococcus faecalis and streptococcus pyogenes with regard to plasmid hmology and resistance inducibility. Antimicrob Agents Chemother. 1975 Jun;7(6):871–873. doi: 10.1128/aac.7.6.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. van Embden J., Cohen S. N. Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids. J Bacteriol. 1973 Nov;116(2):699–709. doi: 10.1128/jb.116.2.699-709.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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