Abstract
Methods are described that resulted in the selective release of deoxyribonucleic acid (DNA)-binding factor from the surface of competent pneumococci. The same methods caused a parallel inactivation of the DNA-binding capacity of the extracted bacteria. Genetically or physiologically incompetent pneumococci did not yield binding factor upon exposure to the same methods. The solubilized binding factor appeared to be a protein; it could be assayed by a membrane filter binding procedure. The binding factor had properties reminiscent of those of the DNA receptors of transformable pneumococci (Seto et al., 1975).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anraku Y., Heppel L. A. On the nature of the changes induced in Escherichia coli by osmotic shock. J Biol Chem. 1967 May 25;242(10):2561–2569. [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Legault-Démare J., Desseaux B., Heyman T., Séror S., Ress G. P. Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters. Biochem Biophys Res Commun. 1967 Aug 23;28(4):550–557. doi: 10.1016/0006-291x(67)90349-x. [DOI] [PubMed] [Google Scholar]
- Mosser J. L., Tomasz A. Choline-containing teichoic acid as a structural component of pneumococcal cell wall and its role in sensitivity to lysis by an autolytic enzyme. J Biol Chem. 1970 Jan 25;245(2):287–298. [PubMed] [Google Scholar]
- Seto H., Lopez R., Garrigan O., Tomasz A. Nucleolytic degradation of homologous and heterologous deoxyribonucleic acid molecules at the surface of competent pneumococci. J Bacteriol. 1975 May;122(2):676–685. doi: 10.1128/jb.122.2.676-685.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seto H., Lopez R., Tomasz A. Cell surface-located deoxyribonucleic acid receptors in transformable pneumococci. J Bacteriol. 1975 Jun;122(3):1339–1350. doi: 10.1128/jb.122.3.1339-1350.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seto H., Tomasz A. Early stages in DNA binding and uptake during genetic transformation of pneumococci. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1493–1498. doi: 10.1073/pnas.71.4.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seto H., Tomasz A. Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci. J Bacteriol. 1975 Jan;121(1):344–353. doi: 10.1128/jb.121.1.344-353.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A. Cellular metabolism in genetic transformation of pneumococci: requirement for protein synthesis during induction of competence. J Bacteriol. 1970 Mar;101(3):860–871. doi: 10.1128/jb.101.3.860-871.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A., Westphal M. Abnormal autolytic enzyme in a pneumococus with altered teichoic acid composition. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2627–2630. doi: 10.1073/pnas.68.11.2627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ziegler R., Tomasz A. Binding of the competence factor to receptors in the spheroplast membrane of pneumococci. Biochem Biophys Res Commun. 1970 Dec 9;41(5):1342–1349. doi: 10.1016/0006-291x(70)90236-6. [DOI] [PubMed] [Google Scholar]