Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Dec;124(3):1153–1158. doi: 10.1128/jb.124.3.1153-1158.1975

Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome.

T J Foster, T G Howe, K M Richmond
PMCID: PMC236021  PMID: 1104574

Abstract

Pairs of normally incompatible derivatives of R100-1 (one ChlS TetR, the other ChilR TetS) were forced to coexist in a recA host by selection for ChlR TetR cells. After many generations stable derivatives were isolated. The analysis of none independent stabilization experiments showed that in each case TetR was translocated from the plasmid to the chromosome of the host. No evidence for the joint integration of other plasmid genes (those controlling transfer, antibiotic resistance, incompatibility, or origin of transfer replication) was obtained. One of the chromosomal TetR determinants was mapped close to metE.

Full text

PDF
1153

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg D. E., Davies J., Allet B., Rochaix J. D. Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628–3632. doi: 10.1073/pnas.72.9.3628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chan R. K., Botstein D., Watanabe T., Ogata Y. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology. 1972 Dec;50(3):883–898. doi: 10.1016/0042-6822(72)90442-4. [DOI] [PubMed] [Google Scholar]
  4. DUBNAU E., STOCKER B. A. GENETICS OF PLASMIDS IN SALMONELLA TYPHIMURIUM. Nature. 1964 Dec 12;204:1112–1113. doi: 10.1038/2041112a0. [DOI] [PubMed] [Google Scholar]
  5. DeVries J. K., Maas W. K. Description of an incompatibility mutant of Escherichia coli. J Bacteriol. 1973 Jul;115(1):213–220. doi: 10.1128/jb.115.1.213-220.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Foster T. J., Howe T. G. Deletion map of the chloramphenicol resistance region of R1 and R100-1. J Bacteriol. 1973 Nov;116(2):1062–1063. doi: 10.1128/jb.116.2.1062-1063.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Foster T. J., Howe T. G. Recombination and complementation between R factors in Escheichia coli K 12. Genet Res. 1971 Dec;18(3):287–297. doi: 10.1017/s0016672300012696. [DOI] [PubMed] [Google Scholar]
  8. Foster T. J. Tetracycline-sensitive mutants of the F-like R factors R100 and R100-1. Mol Gen Genet. 1975;137(1):85–88. doi: 10.1007/BF00332542. [DOI] [PubMed] [Google Scholar]
  9. Foster T. J., Walsh A. Phenotypic characterization of R-factor tetracycline resistance determinants. Genet Res. 1974 Dec;24(3):333–343. doi: 10.1017/s0016672300015330. [DOI] [PubMed] [Google Scholar]
  10. Hashimoto H., Hirota Y. Gene recombination and segregation of resistance factor R in Escherichia coli. J Bacteriol. 1966 Jan;91(1):51–62. doi: 10.1128/jb.91.1.51-62.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  12. Heffron F., Rubens C., Falkow S. Translocation of a plasmid DNA sequence which mediates ampicillin resistance: molecular nature and specificity of insertion. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623–3627. doi: 10.1073/pnas.72.9.3623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Heffron F., Sublett R., Hedges R. W., Jacob A., Falkow S. Origin of the TEM-beta-lactamase gene found on plasmids. J Bacteriol. 1975 Apr;122(1):250–256. doi: 10.1128/jb.122.1.250-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kopecko D. J., Cohen S. N. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373–1377. doi: 10.1073/pnas.72.4.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  16. Maas W. K., Goldschmidt A. D. A mutant of Escherichia coli permitting replication of two F factors. Proc Natl Acad Sci U S A. 1969 Mar;62(3):873–880. doi: 10.1073/pnas.62.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nishimura A., Nishimura Y., Caro L. Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction. J Bacteriol. 1973 Dec;116(3):1107–1112. doi: 10.1128/jb.116.3.1107-1112.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Palchaudhuri S. R., Mazaitis A. J., Maas W. K., Kleinschmidt A. K. Characterization by electron microscopy of fused F-prime factors in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1873–1876. doi: 10.1073/pnas.69.7.1873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Palchoudhury S. R., Iyer V. N. Compatibility between two F' factors in an Escherichia coli strain bearing a chromosomal mutation affecting DNA synthesis. J Mol Biol. 1971 Apr 28;57(2):319–333. doi: 10.1016/0022-2836(71)90349-4. [DOI] [PubMed] [Google Scholar]
  21. Ptashne K., Cohen S. N. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications. J Bacteriol. 1975 May;122(2):776–781. doi: 10.1128/jb.122.2.776-781.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reif H. J., Saedler H. IS1 is involved in deletion formation in the gal region of E. coli K12. Mol Gen Genet. 1975;137(1):17–28. doi: 10.1007/BF00332538. [DOI] [PubMed] [Google Scholar]
  23. San Blas F., Thompson R., Broda P. An Escherichia coli K12 mutant apparently carrying two autonomous F-prime factors. Mol Gen Genet. 1974 May 21;130(2):153–163. doi: 10.1007/BF00269086. [DOI] [PubMed] [Google Scholar]
  24. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  25. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tschäpe H. "Tet-gene pick up" durch ein F-Plasmid in Proteus mirabilis. Z Allg Mikrobiol. 1973;13(8):693–700. doi: 10.1002/jobm.3630130807. [DOI] [PubMed] [Google Scholar]
  27. Tye B. K., Chan R. K., Botstein D. Packaging of an oversize transducing genome by Salmonella phage P22. J Mol Biol. 1974 Jan 5;85(4):485–500. doi: 10.1016/0022-2836(74)90311-8. [DOI] [PubMed] [Google Scholar]
  28. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. III. Transduotion of resistance factors. J Bacteriol. 1961 Aug;82:202–209. doi: 10.1128/jb.82.2.202-209.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watanabe T., Ogata Y., Chan R. K., Botstein D. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22. Virology. 1972 Dec;50(3):874–882. doi: 10.1016/0042-6822(72)90441-2. [DOI] [PubMed] [Google Scholar]
  30. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Willetts N., Bastarrachea F. Genetic and physicochemical characterization of Escherichia coli strains carrying fused F' elements derived from KLF1 and F57. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1481–1485. doi: 10.1073/pnas.69.6.1481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Willetts N. Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12. J Bacteriol. 1974 Jun;118(3):778–782. doi: 10.1128/jb.118.3.778-782.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES