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. 1975 Dec;124(3):1522–1528. doi: 10.1128/jb.124.3.1522-1528.1975

Lipid-phase transitions of the strictly anaerobic bacteria Veillonella parvula and Anaerovibrio lipolytica.

A J Verkley, P H Ververgaert, R A Prins, L M van Golde
PMCID: PMC236067  PMID: 1194242

Abstract

As a basis for physicochemical studies on the membranes of the strictly anaerobic bacteria Veillonella parvula, Anaerovibrio lipolytica, and Megasphaera elsdenii, the fatty acyl and alk-1-enyl moieties on the phosphoglycerides of these organism were characterized. Uncommon is the high proportion of a heptadecenoic acyl and alk-1-enyl moiety in these three lactate-fermenting bacteria. In contrast to V. parvula and A. lipolytica, M. elsdenii contains high amounts of branched-chain acyl and alk-1-enyl moieties. Freeze-etching electron microscopy showed that the lipids of the plasma membranes of V. parvula and A. lipolytica go from the liquid crystalline to the gel state upon lowering of the temperature, indicating that the membrane lipids are predominantly in the fluid state. No lipid-protein segregation could be detected in the plasma membrane of M. elsdenii. This can be explained by the abundance of branched-chain fatty acyl and alk-1-enyl residues in the membranes of this organism which may prevent lipid-protein segregation during the lipid-phase transition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broekhuyse R. M. Phospholipids in tissues of the eye. I. Isolation, characterization and quantitative analysis by two-dimensional thin-layer chromatography of diacyl and vinyl-ether phospholipids. Biochim Biophys Acta. 1968 Mar 4;152(2):307–315. doi: 10.1016/0005-2760(68)90038-6. [DOI] [PubMed] [Google Scholar]
  2. Engelman D. M. Lipid bilayer structure in the membrane of Mycoplasma laidlawii. J Mol Biol. 1971 May 28;58(1):153–165. doi: 10.1016/0022-2836(71)90238-5. [DOI] [PubMed] [Google Scholar]
  3. Esfahani M., Limbrick A. R., Knutton S., Oka T., Wakil S. J. The molecular organization of lipids in the membrane of Escherichia coli: phase transitions. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3180–3184. doi: 10.1073/pnas.68.12.3180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goldfine H. Comparative aspects of bacterial lipids. Adv Microb Physiol. 1972;8:1–58. doi: 10.1016/s0065-2911(08)60187-3. [DOI] [PubMed] [Google Scholar]
  5. Goldfine H., Panos C. Phospholipids of Clostridium butyricum. IV. Analysis of the positional isomers of monounsaturated and cyclopropane fatty acids and alk-1'-enyl ethers by capillary column chromatography. J Lipid Res. 1971 Mar;12(2):214–220. [PubMed] [Google Scholar]
  6. Haest C. W., Verkleij A. J., De Gier J., Scheek R., Ververgaert P. H., Van Deenen L. L. The effect of lipid phase transitions on the architecture of bacterial membranes. Biochim Biophys Acta. 1974 Jul 12;356(1):17–26. doi: 10.1016/0005-2736(74)90290-9. [DOI] [PubMed] [Google Scholar]
  7. JAMES A. T., MARTIN A. J. P. Gas-liquid partition chromatography; the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid. Biochem J. 1952 Mar;50(5):679–690. doi: 10.1042/bj0500679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. II. BIOSYNTHESIS OF PHOSPHOLIPIDS IN ESCHERICHIA COLI. J Biol Chem. 1964 Jun;239:1720–1726. [PubMed] [Google Scholar]
  9. Kates M., Wassef M. K. Lipid chemistry. Annu Rev Biochem. 1970;39:323–358. doi: 10.1146/annurev.bi.39.070170.001543. [DOI] [PubMed] [Google Scholar]
  10. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  11. Prins R. A., Akkermans-Kruyswijk J., Franklin-Klein W., Lankhorst A., van Golde L. M. Metabolism of serine and ethanolamine plasmalogens in Megasphaera elsdenii. Biochim Biophys Acta. 1974 Jun 26;348(3):361–369. doi: 10.1016/0005-2760(74)90216-1. [DOI] [PubMed] [Google Scholar]
  12. Prins R. A., Lankhorst A., van der Meer P., Van Nevel C. J. Some characteristics of Anaerovibrio lipolytica a rumen lipolytic organism. Antonie Van Leeuwenhoek. 1975;41(1):1–11. doi: 10.1007/BF02565031. [DOI] [PubMed] [Google Scholar]
  13. Randle C. L., Albro P. W., Dittmer J. C. The phosphoglyceride composition of Gram-negative bacteria and the changes in composition during growth. Biochim Biophys Acta. 1969;187(2):214–220. doi: 10.1016/0005-2760(69)90030-7. [DOI] [PubMed] [Google Scholar]
  14. Singh H., Spritz N., Geyer B. Studies of brain myelin in the "quaking mouse". J Lipid Res. 1971 Jul;12(4):473–481. [PubMed] [Google Scholar]
  15. Steim J. M., Tourtellotte M. E., Reinert J. C., McElhaney R. N., Rader R. L. Calorimetric evidence for the liquid-crystalline state of lipids in a biomembrane. Proc Natl Acad Sci U S A. 1969 May;63(1):104–109. doi: 10.1073/pnas.63.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Van Golde L. M., Akkermans-Kruyswijk J., Franklin-Klein W., Lankhorst A., Prins R. A. Accumulation of phosphatidylserine in strictly anaerobic lactate fermenting bacteria. FEBS Lett. 1975 Apr 15;53(1):57–60. doi: 10.1016/0014-5793(75)80681-8. [DOI] [PubMed] [Google Scholar]
  17. Verkleij A. J., Ververgaert P. H., van Deenen L. L., Elbers P. F. Phase transitions of phospholipid bilayers and membranes of Acholeplasma laidlawii B visualized by freeze fracturing electron microscopy. Biochim Biophys Acta. 1972 Nov 2;288(2):326–332. doi: 10.1016/0005-2736(72)90253-2. [DOI] [PubMed] [Google Scholar]
  18. de Kruyff B., Demel R. A., van Deenen L. L. The effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact Acholeplasma laidlawii cell membranes and derived liposomes. Biochim Biophys Acta. 1972 Jan 17;255(1):331–347. doi: 10.1016/0005-2736(72)90032-6. [DOI] [PubMed] [Google Scholar]
  19. van Golde L. M. Metabolism of membrane phospholipids and its relation to a novel class of oligosaccharides in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1368–1372. doi: 10.1073/pnas.70.5.1368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. van Golde L. M., Prins R. A., Franklin-Klein W., Akkermans-Kruyswijk J. Phosphatidylserine and its plasmalogen analogue as major lipid constituents in Megasphaera elsdenii. Biochim Biophys Acta. 1973 Dec 20;326(3):314–324. doi: 10.1016/0005-2760(73)90133-1. [DOI] [PubMed] [Google Scholar]
  21. van Golde L. M., van Deenen L. L. The effect of dietary fat on the molecular species of lecithin from rat liver. Biochim Biophys Acta. 1966 Dec 7;125(3):496–509. doi: 10.1016/0005-2760(66)90038-5. [DOI] [PubMed] [Google Scholar]

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