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. 1976 Feb;125(2):608–615. doi: 10.1128/jb.125.2.608-615.1976

Derepression of certain aromatic amino acid biosynthetic enzymes of Escherichia coli K-12 by growth in Fe3+-deficient medium.

J W McCray Jr, K M Herrmann
PMCID: PMC236122  PMID: 1383

Abstract

3-Deoxy-arabino-heptulosonic acid 7-phosphate synthase, prephenate dehydratase, tryptophan synthase, and 2,3-dihydroxybenzoylserine synthase enzyme activities are derepressed in wild-type Escherichia coli K-12 cells grown on Fe3+-deficient medium. This derepression is reversed when FeSO4 is added to the growth medium. Addition of shikimic acid to the Fe3+-deficient growth medium caused repression of the first three enzyme activities but not of 2,3-dihydroxybenzoylserine synthase activity. Addition of 2,3-dihydroxybenzoic acid to the Fe3+-deficient growth medium has no effect on any of the above-mentioned enzyme activities. The Fe3+ deficiency-mediated derepression of 3-deoxyarabino-heptulosonic acid 7-phosphate synthase activity is due to an elevation of the tyrosine-sensitive isoenzyme; the phenylalanine-sensitive isoenzyme is not derepressed under these conditions.

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Selected References

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  1. Brot N., Goodwin J., Fales H. In vivo and in vitro formation of 2,3-dihydroxybenzoylserine by Escherichia coli K12. Biochem Biophys Res Commun. 1966 Nov 22;25(4):454–461. doi: 10.1016/0006-291x(66)90227-0. [DOI] [PubMed] [Google Scholar]
  2. Brot N., Goodwin J. Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. J Biol Chem. 1968 Feb 10;243(3):510–513. [PubMed] [Google Scholar]
  3. Brown K. D., Doy C. H. Control of three isoenzymic 7-phospho-2-oxo-3-deoxy-Darabino-heptonate-D-erythrose-4-phosphate lyases of Escherichia coli W and derived mutants by repressive and "inductive" effects of the aromatic amino acids. Biochim Biophys Acta. 1966 Apr 12;118(1):157–172. doi: 10.1016/s0926-6593(66)80153-4. [DOI] [PubMed] [Google Scholar]
  4. Brown K. D. Regulation of aromatic amino acid biosynthesis Escherichia coli K12. Genetics. 1968 Sep;60(1):31–48. doi: 10.1093/genetics/60.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryce G. F., Brot N. Studies on the enzymatic synthesis of the cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine in Escherichia coli. Biochemistry. 1972 Apr 25;11(9):1708–1715. doi: 10.1021/bi00759a028. [DOI] [PubMed] [Google Scholar]
  6. Bryce G. F., Weller R., Brot N. Studies on the enzymatic synthesis of 2,3-dihydroxy-N-benzoyl-L-serine in Escherichia coli. Biochem Biophys Res Commun. 1971 Mar 5;42(5):871–879. doi: 10.1016/0006-291x(71)90511-0. [DOI] [PubMed] [Google Scholar]
  7. COTTON R. G., GIBSON F. THE BIOSYNTHESIS OF PHENYLALANINE AND TYROSINE; ENZYMES CONVERTING CHORISMIC ACID INTO PREPHENIC ACID AND THEIR RELATIONSHIPS TO PREPHENATE DEHYDRATASE AND PREPHENATE DEHYDROGENASE. Biochim Biophys Acta. 1965 Apr 12;100:76–88. doi: 10.1016/0304-4165(65)90429-0. [DOI] [PubMed] [Google Scholar]
  8. Downer D. N., Davis W. B., Byers B. R. Repression of phenolic acid-synthesizing enzymes and its relation to iron uptake in Bacillus subtilis. J Bacteriol. 1970 Jan;101(1):181–187. doi: 10.1128/jb.101.1.181-187.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  10. Gibson F. Chorismic acid: purification and some chemical and physical studies. Biochem J. 1964 Feb;90(2):256–261. doi: 10.1042/bj0900256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herrmann K. M., Poling M. D. The synthesis of 3-deoxyheptulosonic acid 7-phosphate. J Biol Chem. 1975 Sep 10;250(17):6817–6821. [PubMed] [Google Scholar]
  12. Hua S. S., Markovitz A. Multiple regulator gene control of the galactose operon in Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):1089–1099. doi: 10.1128/jb.110.3.1089-1099.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meers J. L., Tempest D. W., Brown C. M. 'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria. J Gen Microbiol. 1970 Dec;64(2):187–194. doi: 10.1099/00221287-64-2-187. [DOI] [PubMed] [Google Scholar]
  16. Miller R. E., Stadtman E. R. Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. J Biol Chem. 1972 Nov 25;247(22):7407–7419. [PubMed] [Google Scholar]
  17. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. O'Brien I. G., Cox G. B., Gibson F. Biologically active compounds containing 2,3-dihydroxybenzoic acid and serine formed by Escherichia coli. Biochim Biophys Acta. 1970 Mar 24;201(3):453–460. doi: 10.1016/0304-4165(70)90165-0. [DOI] [PubMed] [Google Scholar]
  19. O'Brien I. G., Gibson F. The structure of enterochelin and related 2,3-dihydroxy-N-benzoylserine conjugates from Escherichia coli. Biochim Biophys Acta. 1970 Aug 14;215(2):393–402. doi: 10.1016/0304-4165(70)90038-3. [DOI] [PubMed] [Google Scholar]
  20. Porra R. J., Langman L., Young I. G., Gibson F. The role of ferric enterochelin esterase in enterochelin-mediated iron transport and ferrochelatase activity in Escherichia coli. Arch Biochem Biophys. 1972 Nov;153(1):74–78. doi: 10.1016/0003-9861(72)90422-5. [DOI] [PubMed] [Google Scholar]
  21. RACKER E., KLYBAS V., SCHRAMM M. Tetrose diphosphate, a specific inhibitor of glyceraldehyde 3-phosphate dehydrogenase. J Biol Chem. 1959 Oct;234:2510–2516. [PubMed] [Google Scholar]
  22. Rosenberg A. H., Gefter M. L. An iron-dependent modification of several transfer RNA species in Escherichia coli. J Mol Biol. 1969 Dec 28;46(3):581–584. doi: 10.1016/0022-2836(69)90197-1. [DOI] [PubMed] [Google Scholar]
  23. Sieben A. S., Perlin A. S., Simpson F. J. An improved preparative method for D-erythrose 4-phosphate. Can J Biochem. 1966 Jun;44(6):663–669. doi: 10.1139/o66-083. [DOI] [PubMed] [Google Scholar]
  24. Yost F. J., Jr, Fridovich I. Superoxide radicals and phagocytosis. Arch Biochem Biophys. 1974 Apr 2;161(2):395–401. doi: 10.1016/0003-9861(74)90320-8. [DOI] [PubMed] [Google Scholar]

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