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. 1976 Mar;125(3):845–849. doi: 10.1128/jb.125.3.845-849.1976

Adaptation of a stable L-form of Bacillus subtilis to minimal salts medium without osmotic stabilizers.

R W Gilpin, S K Patterson
PMCID: PMC236157  PMID: 815249

Abstract

An L-form isolated from Bacillus subtilis 168 was adapted to growth in a 340 mOsm minimal salts medium without the addition of osmotically protective solutes. This L-form had no chemically detectable peptidoglycan residues on its surface, but 0.8% of the dry weight of washed membranes was hexosamine. The osmotic stability and susceptibility to bacitracin and vancomycin of the L-form adapted to growth in 340 mOsm osmotically unprotected medium was twice that of the L-form grown in 2,680 mOsm medium supplemented with 1.2 M NaCl.

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Selected References

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