Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1976 Mar;125(3):872–879. doi: 10.1128/jb.125.3.872-879.1976

In vitro system for the synthesis of teichoic acid linked to peptidoglycan.

R Bracha, L Glaser
PMCID: PMC236161  PMID: 1254557

Abstract

A crude cell wall preparation from Staphylococcus aureus H prepared by the method of Mirelman and Sharon (1972) was shown to catalyze the synthesis of polyribitol phosphate linked to the cell wall peptidoglycan. The reaction used cytidine diphosphate (CDP)-ribitol as a substrate and in addition required the presence of CDP-glycerol, uridine diphosphate (UDP)-N-acetyl-D-glucosamine, and adenosine triphosphate. Incubation of radioactive CDP-glycerol with the crude cell wall preparation resulted in the transfer of glycerol phosphate residues to the cell wall; this reaction was greatly stimulated by the presence of UDP-N-acetylglucosamine. These data suggest that polyribitol phosphate is linked to the cell wall peptidoglycan by an oligomer contaning N-acetyl-D-glucosamine and glycerol phosphate.

Full text

PDF
872

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGER M. M., GLASER L. THE SYNTHESIS OF TEICHOIC ACIDS. I. POLYGLYCEROPHOSPHATE. J Biol Chem. 1964 Oct;239:3168–3177. [PubMed] [Google Scholar]
  2. CHATTERJEE A. N., PARK J. T. BIOSYNTHESIS OF CELL WALL MUCOPEPTIDE BY A PARTICULATE FRACTION FROM STAPHYLOCOCCUS AUREUS. Proc Natl Acad Sci U S A. 1964 Jan;51:9–16. doi: 10.1073/pnas.51.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chatterjee A. N. Use of bacteriophage-resistant mutants to study the nature of the bacteriophage receptor site of Staphylococcus aureus. J Bacteriol. 1969 May;98(2):519–527. doi: 10.1128/jb.98.2.519-527.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fiedler F., Glaser L. The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier. J Biol Chem. 1974 May 10;249(9):2684–2689. [PubMed] [Google Scholar]
  5. Fiedler F., Glaser L. The synthesis of polyribitol phosphate. II. On the mechanism of polyribitol phosphate polymerase. J Biol Chem. 1974 May 10;249(9):2690–2695. [PubMed] [Google Scholar]
  6. GLASER L., BURGER M. M. THE SYNTHESIS OF TEICHOIC ACIDS. 3. GLUCOSYLATION OF POLYGLYCEROPHOSPHATE. J Biol Chem. 1964 Oct;239:3187–3191. [PubMed] [Google Scholar]
  7. GLASER L. THE SYNTHESIS OF TEICHOIC ACIDS. II. POLYRIBITOL PHOSPHATE. J Biol Chem. 1964 Oct;239:3178–3186. [PubMed] [Google Scholar]
  8. Ghuysen J. M. Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriol Rev. 1968 Dec;32(4 Pt 2):425–464. [PMC free article] [PubMed] [Google Scholar]
  9. Hancock I., Baddiley J. In vitro synthesis of the unit that links teichoic acid to peptidoglycan. J Bacteriol. 1976 Mar;125(3):880–886. doi: 10.1128/jb.125.3.880-886.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heckels J. E., Archibald A. R., Baddiley J. Studies on the linkage between teichoic acid and peptidoglycan in a bacteriophage-resistant mutant of Staphylococcus aureus H. Biochem J. 1975 Sep;149(3):637–647. doi: 10.1042/bj1490637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herbold D. R., Glaser L. Bacillus subtilis N-acetylmuramic acid L-alanine amidase. J Biol Chem. 1975 Mar 10;250(5):1676–1682. [PubMed] [Google Scholar]
  12. Mauck J., Glaser L. On the mode of in vivo assembly of the cell wall of Bacillus subtilis. J Biol Chem. 1972 Feb 25;247(4):1180–1187. [PubMed] [Google Scholar]
  13. Mirelman D., Sharon N. Biosynthesis of peptidoglycan by a cell wall preparation of Staphylococcus aureus and its inhibition by penicillin. Biochem Biophys Res Commun. 1972 Mar 10;46(5):1909–1917. doi: 10.1016/0006-291x(72)90069-1. [DOI] [PubMed] [Google Scholar]
  14. Mirelman D., Shaw D. R., Park J. T. Nature and origins of phosphorus compounds in isolated cell walls of Staphylococcus aureus. J Bacteriol. 1971 Jul;107(1):239–244. doi: 10.1128/jb.107.1.239-244.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neuhaus F. C., Linzer R., Reusch V. M., Jr Biosynthesis of membrane teichoic acid: role of the D-alanine-activating enzyme and D-alanine: membrane acceptor ligase. Ann N Y Acad Sci. 1974 May 10;235(0):502–518. doi: 10.1111/j.1749-6632.1974.tb43287.x. [DOI] [PubMed] [Google Scholar]
  16. Tomasz A., McDonnell M., Westphal M., Zanati E. Coordinated incorporation of nascent peptidoglycan and teichoic acid into pneumococcal cell walls and conservation of peptidoglycan during growth. J Biol Chem. 1975 Jan 10;250(1):337–341. [PubMed] [Google Scholar]
  17. Wyke A. W., Ward J. B. The synthesis of covalently-linked teichoic acid and peptidoglycan by cell-free preparations of Bacillus licheniformis. Biochem Biophys Res Commun. 1975 Aug 4;65(3):877–885. doi: 10.1016/s0006-291x(75)80467-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES