Abstract
The role of cytidine diphosphate (CDP)-glycerol in gram-positive bacteria whose walls lack poly(glycerol phosphate) was investigated. Membrane preparations from Staphylococcus aureus H, Bacillus subtilis W23, and Micrococcus sp. 2102 catalyzed the incorporation of glycerol phosphate residues from radioactive CDP-glycerol into a water-soluble polymer. In toluenized cells of Micrococcus sp. 2102, some of this product became linked to the wall. In each case, maximum incorporation of glycerol phosphate residues required the presence of the nucleotide precursors of wall teichoic acid and of uridine diphosphate-N-acetylglucosamine. In membrane preparations capable of synthesizing peptidoglycan, vancomycin caused a decrease in the incorporation of isotope from CDP-glycerol into polymer. Synthesis of the poly (glycerol phosphate) unit thus depended at an early stage on the concomitant synthesis of wall teichoic acid and later on the synthesis of peptidoglycan. It is concluded that CDP-glycerol is the biosynthetic precursor of the tri(glycerol phosphate) linkage unit between teichoic acid and peptidoglycan that has recently been characterized in S. aureus H.
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- Anderson J. S., Matsuhashi M., Haskin M. A., Strominger J. L. Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence. J Biol Chem. 1967 Jul 10;242(13):3180–3190. [PubMed] [Google Scholar]
- Anderson R. G., Hussey H., Baddiley J. The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane. Biochem J. 1972 Mar;127(1):11–25. doi: 10.1042/bj1270011. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks D., Baddiley J. The mechanism of biosynthesis and direction of chain extension of a poly-(N-acetylglucosamine 1-phosphate) from the walls of Staphylococcus lactis N.C.T.C. 2102. Biochem J. 1969 Jul;113(4):635–642. doi: 10.1042/bj1130635. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chin T., Burger M. M., Glaser L. Synthesis of teichoic acids. VI. The formation of multiple wall polymers in Bacillus subtilis W-23. Arch Biochem Biophys. 1966 Sep 26;116(1):358–367. doi: 10.1016/0003-9861(66)90042-7. [DOI] [PubMed] [Google Scholar]
- Coley J., Duckworth M., Baddiley J. Extraction and purification of lipoteichoic acids from Gram-positive bacteria. Carbohydr Res. 1975 Mar;40(1):41–52. doi: 10.1016/s0008-6215(00)82667-1. [DOI] [PubMed] [Google Scholar]
- Emdur L. I., Chiu T. H. Turnover of phosphatidylglycerol in Streptococcus sanguis. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1137–1144. doi: 10.1016/s0006-291x(74)80097-5. [DOI] [PubMed] [Google Scholar]
- Emdur L., Chiu T. The role of phosphatidylglycerol in the in vitro biosynthesis of teichoic acid and lipoteichoic acid. FEBS Lett. 1975 Jul 15;55(1):216–219. doi: 10.1016/0014-5793(75)80995-1. [DOI] [PubMed] [Google Scholar]
- Fiedler F., Glaser L. The synthesis of polyribitol phosphate. I. Purification of polyribitol phosphate polymerase and lipoteichoic acid carrier. J Biol Chem. 1974 May 10;249(9):2684–2689. [PubMed] [Google Scholar]
- GLASER L. THE SYNTHESIS OF TEICHOIC ACIDS. II. POLYRIBITOL PHOSPHATE. J Biol Chem. 1964 Oct;239:3178–3186. [PubMed] [Google Scholar]
- Glaser L., Lindsay B. The synthesis of lipoteichoic acid carrier. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1131–1136. doi: 10.1016/s0006-291x(74)80096-3. [DOI] [PubMed] [Google Scholar]
- Heckels J. E., Archibald A. R., Baddiley J. Studies on the linkage between teichoic acid and peptidoglycan in a bacteriophage-resistant mutant of Staphylococcus aureus H. Biochem J. 1975 Sep;149(3):637–647. doi: 10.1042/bj1490637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHAW D. R. Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol. Biochem J. 1962 Feb;82:297–312. doi: 10.1042/bj0820297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schrader W. P., Fan D. P. Synthesis of cross-linked peptidoglycan attached to previously formed cell wall by toluene-treated cells of Bacillus megaterium. J Biol Chem. 1974 Aug 10;249(15):4815–4818. [PubMed] [Google Scholar]
- Warner J. R., Morgan S. A., Shulman R. W. Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae. J Bacteriol. 1976 Mar;125(3):887–891. doi: 10.1128/jb.125.3.887-891.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]