Abstract
A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine is reduced by half within 20 s. During that period there is a substantial expansion of the pool. A logarithmically growing cell in synthetic medium contains about 2 X 10(6) molecules of S-adenosylmethionine, of which only 10% is used for the methylation of ribonucleic acid molecules.
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Selected References
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- Boehlke K. W., Friesen J. D. Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate. J Bacteriol. 1975 Feb;121(2):429–433. doi: 10.1128/jb.121.2.429-433.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chakraborty P. R., Bandyopadhyay P., Huang H. H., Maitra U. Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase. J Biol Chem. 1974 Nov 10;249(21):6901–6909. [PubMed] [Google Scholar]
- Getz G. S., Jakovcic S., Heywood J., Frank J., Rabinowitz M. A two-dimensional thin-layer chromatographic system for phospholipid separation. The analysis of yeast phospholipids. Biochim Biophys Acta. 1970 Dec 15;218(3):441–452. doi: 10.1016/0005-2760(70)90007-x. [DOI] [PubMed] [Google Scholar]
- Holcomb E. R., Shapiro S. K. Assay and regulation of S-adenosylmethionine synthetase in Saccharomyces cerevisiae and Candida utilis. J Bacteriol. 1975 Jan;121(1):267–271. doi: 10.1128/jb.121.1.267-271.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klootwijk J., Planta R. J. Analysis of the methylation sites in yeast ribosomal RNA. Eur J Biochem. 1973 Nov 15;39(2):325–333. doi: 10.1111/j.1432-1033.1973.tb03130.x. [DOI] [PubMed] [Google Scholar]
- PARKS L. W., SCHLENK F. Formation of alpha-amino-gamma-butyrolactone from S-adenosylmethionine. Arch Biochem Biophys. 1958 May;75(1):291–292. doi: 10.1016/0003-9861(58)90419-3. [DOI] [PubMed] [Google Scholar]
- Pawlowski P. J., Vaughan M. H. Comparison of the relative synthesis of the proteins of the 50S ribosomal subunit in growing and valine-deprived HeLa cells. J Cell Biol. 1972 Feb;52(2):409–419. doi: 10.1083/jcb.52.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHLENK F., DEPALMA R. E. The formation of S-adenosylmethionine in yeast. J Biol Chem. 1957 Dec;229(2):1037–1050. [PubMed] [Google Scholar]
- Schlenk F., Zydek-Cwick C. R. Stabilit of the glycosidic bond of S-adenosylsulfonium compounds toward acid. Arch Biochem Biophys. 1969 Nov;134(2):414–422. doi: 10.1016/0003-9861(69)90301-4. [DOI] [PubMed] [Google Scholar]
- Udem S. A., Warner J. R. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. doi: 10.1016/0022-2836(72)90279-3. [DOI] [PubMed] [Google Scholar]
- Warner J. R. The assembly of ribosomes in yeast. J Biol Chem. 1971 Jan 25;246(2):447–454. [PubMed] [Google Scholar]
- Warner J. R., Udem S. A. Temperature sensitive mutations affecting ribosome synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):243–257. doi: 10.1016/0022-2836(72)90280-x. [DOI] [PubMed] [Google Scholar]