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. 1976 Mar;125(3):887–891. doi: 10.1128/jb.125.3.887-891.1976

Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.

J R Warner, S A Morgan, R W Shulman
PMCID: PMC236163  PMID: 767330

Abstract

A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine is reduced by half within 20 s. During that period there is a substantial expansion of the pool. A logarithmically growing cell in synthetic medium contains about 2 X 10(6) molecules of S-adenosylmethionine, of which only 10% is used for the methylation of ribonucleic acid molecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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