Abstract
Polymeric carbohydrates containing glycerol and fatty acids were isolated from whole cells and membranes of mycoplasmas by hot aqueous phenol extraction and gel filtration. Lipopolysaccharides were found to occur in four species of Acholeplasma, two of Anaeroplasma, and in Mycoplasma neurolyticum. None were detected in Spiroplasma citri or in five species of Mycoplasma. All lipopolysaccharides contained both neutral and N-acylated amino sugars in ratios varying from 1:1 to 3:1. The neutral sugars found in varying distribution were glucose, galactose, and mannose. The amino sugars included fucosamine, an unidentified deoxyhexosamine, galactosamine, and glucosamine. Fucosamine and glucose were the only sugars common to all lipopolysaccharides. The fatty acids were similar to those found in the lipids of each organism.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
- Beckman B. L., Kenny G. E. Immunochemical analysis of serologically active lipids of Mycoplasma pneumoniae. J Bacteriol. 1968 Oct;96(4):1171–1180. doi: 10.1128/jb.96.4.1171-1180.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRUMPTON M. J., DAVIES D. A. The isolation of D-fucosamine from the specific polysaccharide of Chromobacterium violaceum (NCTC 7917). Biochem J. 1958 Dec;70(4):729–736. doi: 10.1042/bj0700729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRUMPTON M. J. Identification of amino sugars. Biochem J. 1959 Jul;72:479–486. doi: 10.1042/bj0720479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilliam J. M., Morowitz H. J. Characterization of the plasma membrane of Mycoplasma laidlawii. IX. Isolation and characterization of the membrane polyhexosamine. Biochim Biophys Acta. 1972 Aug 9;274(2):353–363. doi: 10.1016/0005-2736(72)90183-6. [DOI] [PubMed] [Google Scholar]
- Hollingdale M. R., Lemcke R. M. Membrane antigens of Mycoplasma hominis. J Hyg (Lond) 1972 Mar;70(1):85–98. doi: 10.1017/s0022172400022129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Langworthy T. A., Mayberry W. R., Smith P. F., Robinson I. M. Plasmalogen composition of Anaeroplasma. J Bacteriol. 1975 May;122(2):785–787. doi: 10.1128/jb.122.2.785-787.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Langworthy T. A., Smith P. F., Mayberry W. R. Lipids of Thermoplasma acidophilum. J Bacteriol. 1972 Dec;112(3):1193–1200. doi: 10.1128/jb.112.3.1193-1200.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayberry-Carson K. J., Langworthy T. A., MAyberry W. R., Smith P. F. A new class of lipopolysaccharide from Thermoplasma acidophilum. Biochim Biophys Acta. 1974 Aug 22;360(2):217–229. doi: 10.1016/0005-2760(74)90171-4. [DOI] [PubMed] [Google Scholar]
- Mayberry-Carson K. J., Roth I. L., Smith P. F. Ultrastructure of lipopolysaccharide isolated from Thermoplasma acidophilum. J Bacteriol. 1975 Feb;121(2):700–703. doi: 10.1128/jb.121.2.700-703.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayberry W. R., Smith P. F., Langworthy T. A. Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum. J Bacteriol. 1974 Jun;118(3):898–904. doi: 10.1128/jb.118.3.898-904.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayberry W. R., Smith P. F., Langworthy T. A., Plackett P. Identification of the amide-linked fatty acids of Acholeplasma axanthum S743 as D(-)3-hydroxyhexadecanoate and its homologues. J Bacteriol. 1973 Dec;116(3):1091–1095. doi: 10.1128/jb.116.3.1091-1095.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plackett P., Buttery S. H. A galactofuranose disaccharide from the galactan of Mycoplasma mycoides. Biochem J. 1964 Jan;90(1):201–205. doi: 10.1042/bj0900201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plackett P., Marmion B. P., Shaw E. J., Lemcke R. M. Immunochemical analysis of Mycoplasma pneumoniae. 3. Separation and chemical identification of serologically active lipids. Aust J Exp Biol Med Sci. 1969 Apr;47(2):171–195. doi: 10.1038/icb.1969.19. [DOI] [PubMed] [Google Scholar]
- Plackett P., Shaw E. J. Glucolipids from Mycoplasma laidlawii and Streptococcus MG. Biochem J. 1967 Sep;104(3):61C–62C. doi: 10.1042/bj1040061c. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSEN H. A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957 Mar;67(1):10–15. doi: 10.1016/0003-9861(57)90241-2. [DOI] [PubMed] [Google Scholar]
- Schiefer H. G., Gerhardt U., Brunner H., Krüpe M. Studies with lectins on the surface carbohydrate structures of mycoplasma membranes. J Bacteriol. 1974 Oct;120(1):81–88. doi: 10.1128/jb.120.1.81-88.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith P. F. Lipid composition of Mycoplasma neurolyticum. J Bacteriol. 1972 Oct;112(1):554–558. doi: 10.1128/jb.112.1.554-558.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugiyama T., Smith P. F., Langworthy T. A., Mayberry W. R. Immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas. Infect Immun. 1974 Dec;10(6):1273–1279. doi: 10.1128/iai.10.6.1273-1279.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TULLY J. G. PRODUCTION AND BIOLOGICAL CHARACTERISTICS OF AN EXTRACELLULAR NEUROTOXIN FROM MYCOPLASMA NEUROLYTICUM. J Bacteriol. 1964 Aug;88:381–388. doi: 10.1128/jb.88.2.381-388.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terry T. M., Zupnik J. S. Weak association of glucosamine-containing polymer with the Acholeplasma laidlawii membrane. Biochim Biophys Acta. 1973 Jan 2;291(1):144–148. doi: 10.1016/0005-2736(73)90069-2. [DOI] [PubMed] [Google Scholar]
- Wright D. N. Nature of penicillin-induced growth inhibition of Mycoplasma neurolyticum. J Bacteriol. 1967 Jan;93(1):185–190. doi: 10.1128/jb.93.1.185-190.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]