Abstract
A mutant of Escherichia coli with a new promoter for the lac operon exhibits dramatic discontinuities in the synthesis of lac messenger ribonucleic acid after induction. These discontinuities immediately precede similar discontinuities in the synthesis of beta-galactosidase. The discontinuous synthesis of beta-galactosidase persists after addition of rifampin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beckwith J., Grodzicker T., Arditti R. Evidence for two sites in the lac promoter region. J Mol Biol. 1972 Aug 14;69(1):155–160. doi: 10.1016/0022-2836(72)90031-9. [DOI] [PubMed] [Google Scholar]
- Bruenn J., Hollingsworth H. A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3693–3697. doi: 10.1073/pnas.70.12.3693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruenn J., Le Lucia-Curtin P. Genetic mapping of a new promoter for the lac operon. J Mol Biol. 1975 Apr 5;93(2):311–317. doi: 10.1016/0022-2836(75)90134-5. [DOI] [PubMed] [Google Scholar]
- Darlix J. L., Horaist M. Existence and possible roles of transcriptional barriers in T7 DNA early region as shown by electron microscopy. Nature. 1975 Jul 24;256(5515):288–292. doi: 10.1038/256288a0. [DOI] [PubMed] [Google Scholar]
- De Crombrugghe E., Chen B., Anderson W. B., Gottesman M. E., Perlman R. L., Pastan I. Role of cyclic adenosine 3',5'-monophosphate and the cyclic adenosine 3',5'-monophosphate receptor protein in the initiation of lac transcription. J Biol Chem. 1971 Dec 10;246(23):7343–7348. [PubMed] [Google Scholar]
- KEPES A. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963 Oct 15;76:293–309. [PubMed] [Google Scholar]
- Maizels N. M. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585–3589. doi: 10.1073/pnas.70.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reznikoff W. S., Michels C. A., Cooper T. G., Silverstone A. E., Magasanik B. Inhibition of lacZ gene translation initiation in trp-lac fusion strains. J Bacteriol. 1974 Mar;117(3):1231–1239. doi: 10.1128/jb.117.3.1231-1239.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]