Abstract
Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary forms. With time-lapse photomicrography, reversion sequences of single protoplasts were demonstrated.
Full text
PDF







Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FITZ-JAMES P. C. Cytological and chemical studies of the growth of protoplasts of Bacillus megaterium. J Biophys Biochem Cytol. 1958 May 25;4(3):257–266. doi: 10.1083/jcb.4.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fodor K., Hadlaczky G., Alföldi L. Reversion of Bacillus megaterium protoplasts to the bacillary form. J Bacteriol. 1975 Jan;121(1):390–391. doi: 10.1128/jb.121.1.390-391.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kusaka I. Growth and division of protoplasts of Bacillus megaterium and inhibition of division by penicillin. J Bacteriol. 1967 Oct;94(4):884–888. doi: 10.1128/jb.94.4.884-888.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landman O. E., Ryter A., Fréhel C. Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: electron-microscopic and physical study. J Bacteriol. 1968 Dec;96(6):2154–2170. doi: 10.1128/jb.96.6.2154-2170.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MCQUILLEN K. Bacterial protoplasts: growth and division of protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1955 Nov;18(3):458–461. doi: 10.1016/0006-3002(55)90129-3. [DOI] [PubMed] [Google Scholar]
- Miller I. L., Wiebe W., Landman O. E. Gelatin-induced reversion of protoplasts of Bacillus subtilis to the bacillary form: photomicrographic study. J Bacteriol. 1968 Dec;96(6):2171–2174. doi: 10.1128/jb.96.6.2171-2174.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynolds P. E. Peptidoglycan synthesis in bacilli. II. Characteristics of protoplast membrane preparations. Biochim Biophys Acta. 1971 May 18;237(2):255–272. doi: 10.1016/0304-4165(71)90316-3. [DOI] [PubMed] [Google Scholar]
- WEIBULL C. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J Bacteriol. 1953 Dec;66(6):688–695. doi: 10.1128/jb.66.6.688-695.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]