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. 1976 Mar;125(3):1172–1179. doi: 10.1128/jb.125.3.1172-1179.1976

Morphological study of the reversion to bacillary form, of Bacillus megaterium protoplasts.

G Hadlaczky, K Fodor, L Alföldi
PMCID: PMC236197  PMID: 815240

Abstract

Protoplasts of Bacillus megaterium readily reverted to bacillary form in liquid media and when plated in a soft-agar layer onto the surface of appropriate agar media. Three phases of the reversion sequence could be differentiated by phase contrast microscopy: (i) increase in size of the individual protoplasts, (ii) non oriented division of the protoplasts and (iii) outgrowth of the bacillary forms. With time-lapse photomicrography, reversion sequences of single protoplasts were demonstrated.

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Selected References

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