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. 1976 Mar;125(3):1237–1239. doi: 10.1128/jb.125.3.1237-1239.1976

In Vivo Distribution of Ribonucleic Acid Polymerase Between Cytoplasm and Nucleoid in Escherichia coli

W Rünzi 1, H Matzura 1
PMCID: PMC236211  PMID: 767324

Abstract

Three forms of ribonucleic acid polymerase can be distinguished in exponentially growing Escherichia coli cells: (i) active, (ii) inactive, inside the nucleoid, and (iii) inactive, free in the cytoplasm.

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Selected References

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  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burgess R. R. Separation and characterization of the subunits of ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6168–6176. [PubMed] [Google Scholar]
  3. Dalbow D. G. Synthesis of RNA polymerase in Escherichia coli B-r growing at different rates. J Mol Biol. 1973 Mar 25;75(1):181–184. doi: 10.1016/0022-2836(73)90537-8. [DOI] [PubMed] [Google Scholar]
  4. GOLDSTEIN A., GOLDSTEIN D. B., LOWNEY L. I. PROTEIN SYNTHESIS OF 0 DEGREES C IN ESCHERICHIA COLI. J Mol Biol. 1964 Jul;9:213–235. doi: 10.1016/s0022-2836(64)80102-9. [DOI] [PubMed] [Google Scholar]
  5. Iwakura Y., Ito K., Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. I. Control of RNA polymerase content at various growth rates. Mol Gen Genet. 1974;133(1):1–23. doi: 10.1007/BF00268673. [DOI] [PubMed] [Google Scholar]
  6. Matzura H., Hansen B. S., Zeuthen J. Biosynthesis of the beta and beta' subunits of RNA polymerase in Escherichia coli. J Mol Biol. 1973 Feb 15;74(1):9–20. doi: 10.1016/0022-2836(73)90350-1. [DOI] [PubMed] [Google Scholar]
  7. Matzura H., Molin S., Maaloe O. Sequential biosynthesis of the and ' subunits of the DNA-dependent RNA polymerase from Escherichia coli. J Mol Biol. 1971 Jul 14;59(1):17–25. doi: 10.1016/0022-2836(71)90410-4. [DOI] [PubMed] [Google Scholar]
  8. Oeschger M. P., Berlyn M. K. Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees. Proc Natl Acad Sci U S A. 1975 Mar;72(3):911–915. doi: 10.1073/pnas.72.3.911. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rogerson A. C. Evidence for a cryptic RNA polymerase activity in DNA-free minicells of Escherichia coli. Biochem Biophys Res Commun. 1975 Sep 16;66(2):665–670. doi: 10.1016/0006-291x(75)90561-6. [DOI] [PubMed] [Google Scholar]
  10. Rogerson A. C., Stone J. E. Beta-beta' subunits of ribonucleic acid polymerase in episome-free minicells of Escherichia coli. J Bacteriol. 1974 Jul;119(1):332–333. doi: 10.1128/jb.119.1.332-333.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roozen K. J., Fenwick R. G., Jr, Curtiss R., 3rd Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12. J Bacteriol. 1971 Jul;107(1):21–33. doi: 10.1128/jb.107.1.21-33.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stonington O. G., Pettijohn D. E. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc Natl Acad Sci U S A. 1971 Jan;68(1):6–9. doi: 10.1073/pnas.68.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]

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