Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1999 Sep 24;79(1):54–58. doi: 10.1038/sj.bjc.6690011

Perchloric acid-soluble proteins from goat liver inhibit chemical carcinogenesis of Syrian hamster cheek-pouch carcinoma

F Ghezzo 1, G N Berta 1, B Bussolati 2, A Bosio 1, G Corvetti 1, F Di Carlo 1, G Bussolati 3, R Guglielmone 4, A Bartorelli 4
PMCID: PMC2362162  PMID: 10408693

Abstract

Chemically induced Syrian hamster cheek-pouch squamous cell carcinoma is very similar to the corresponding human tumour. This paper describes a blind study in which inhibition of dimethylbenzanthracene-induced cheek-pouch tumours by a goat liver extract denominated UK101 was investigated. Less than 40% of animals treated with UK101 developed tumours compared with 100% of the controls. Intermediate results (80%) were noted in a positive control group treated with Calmette–Guérin bacillus. Immunocytochemical testing of cheek-pouch mucosa by Mib5 showed significantly less proliferating cells in UK101 animals than in the controls. The effect of UK101 was completely reversed when dexamethasone was added in a third control group. A significant difference in complement-mediated cytotoxicity was noted in the sera of UK101-tested and control animals. These findings suggest that an immune mechanism is responsible for the inhibition of hamster cheek-pouch carcinoma by UK101. © 1999 Cancer Research Campaign

Keywords: Calmette–Guérin bacillus, cytotoxicity, dexamethasone, hamster cheek-pouch carcinoma, Mib5, tumour inhibition; UK101

Full Text

The Full Text of this article is available as a PDF (136.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andronico G., Mangano M., Ferrara L., Lamanna D., Mulé G., Cerasola G. In vivo relationship between insulin and endothelin role of insulin-resistance. J Hum Hypertens. 1997 Jan;11(1):63–66. doi: 10.1038/sj.jhh.1000386. [DOI] [PubMed] [Google Scholar]
  2. Ceciliani F., Faotto L., Negri A., Colombo I., Berra B., Bartorelli A., Ronchi S. The primary structure of UK114 tumor antigen. FEBS Lett. 1996 Sep 16;393(2-3):147–150. doi: 10.1016/0014-5793(96)00850-2. [DOI] [PubMed] [Google Scholar]
  3. Eisenberg E., Shklar G. Levamisole and hamster pouch carcinogenesis. Oral Surg Oral Med Oral Pathol. 1977 Apr;43(4):562–571. doi: 10.1016/0030-4220(77)90109-8. [DOI] [PubMed] [Google Scholar]
  4. Gilmore W., Giunta J. L. The effect of 13-cis-retinoic acid on hamster buccal pouch carcinogenesis. Oral Surg Oral Med Oral Pathol. 1981 Mar;51(3):256–265. doi: 10.1016/0030-4220(81)90055-4. [DOI] [PubMed] [Google Scholar]
  5. Gimenez-Conti I. B., LaBate M., Liu F., Osterndorff E. p53 alterations in chemically induced hamster cheek-pouch lesions. Mol Carcinog. 1996 Aug;16(4):197–202. doi: 10.1002/(SICI)1098-2744(199608)16:4<197::AID-MC3>3.0.CO;2-D. [DOI] [PubMed] [Google Scholar]
  6. Giunta J. L., Reif A. E., Shklar G. Bacillus Calmette-Guérin and antilymphocyte serum in carcinogenesis. Effects on the hamster pouch. Arch Pathol. 1974 Oct;98(4):237–240. [PubMed] [Google Scholar]
  7. Levy-Favatier F., Cuisset L., Nedelec B., Tichonicky L., Kruh J., Delpech M. Characterization, purification and cDNA cloning of a rat perchloric-acid-soluble 23-kDa protein present only in liver and kidney. Eur J Biochem. 1993 Mar 15;212(3):665–673. doi: 10.1111/j.1432-1033.1993.tb17704.x. [DOI] [PubMed] [Google Scholar]
  8. Odukoya O., Shklar G. Two-phase carcinogenesis in hamster buccal pouch. Oral Surg Oral Med Oral Pathol. 1982 Nov;54(5):547–552. doi: 10.1016/0030-4220(82)90193-1. [DOI] [PubMed] [Google Scholar]
  9. Oka T., Tsuji H., Noda C., Sakai K., Hong Y. M., Suzuki I., Muñoz S., Natori Y. Isolation and characterization of a novel perchloric acid-soluble protein inhibiting cell-free protein synthesis. J Biol Chem. 1995 Dec 15;270(50):30060–30067. doi: 10.1074/jbc.270.50.30060. [DOI] [PubMed] [Google Scholar]
  10. Parkin D. M., Pisani P., Ferlay J. Estimates of the worldwide incidence of eighteen major cancers in 1985. Int J Cancer. 1993 Jun 19;54(4):594–606. doi: 10.1002/ijc.2910540413. [DOI] [PubMed] [Google Scholar]
  11. Racca S., Di Carlo F., Bartorelli A., Bussolati B., Bussolati G. Growth inhibition of DMBA-induced rat mammary carcinomas by UK 114. Virchows Arch. 1997 Nov;431(5):323–328. doi: 10.1007/s004280050106. [DOI] [PubMed] [Google Scholar]
  12. SALLEY J. J. Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res. 1954 Apr;33(2):253–262. doi: 10.1177/00220345540330021201. [DOI] [PubMed] [Google Scholar]
  13. Schlüter C., Duchrow M., Wohlenberg C., Becker M. H., Key G., Flad H. D., Gerdes J. The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513–522. doi: 10.1083/jcb.123.3.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schmiedeknecht G., Kerkhoff C., Orsó E., Stöhr J., Aslanidis C., Nagy G. M., Knuechel R., Schmitz G. Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation. Eur J Biochem. 1996 Dec 1;242(2):339–351. doi: 10.1111/j.1432-1033.1996.0339r.x. [DOI] [PubMed] [Google Scholar]
  15. Schwartz J., Shklar G., Reid S., Trickler D. Prevention of experimental oral cancer by extracts of Spirulina-Dunaliella algae. Nutr Cancer. 1988;11(2):127–134. doi: 10.1080/01635588809513979. [DOI] [PubMed] [Google Scholar]
  16. Shklar G. Experimental oral pathology in the Syrian hamster. Prog Exp Tumor Res. 1972;16:518–538. doi: 10.1159/000393387. [DOI] [PubMed] [Google Scholar]
  17. Trickler D., Shklar G. Prevention by vitamin E of experimental oral carcinogenesis. J Natl Cancer Inst. 1987 Jan;78(1):165–169. doi: 10.1093/jnci/78.1.165. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES