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. 1999 Jan;79(2):236–243. doi: 10.1038/sj.bjc.6690039

Substance P activates responses correlated with tumour growth in human glioma cell lines bearing tachykinin NK1 receptors

C Palma 1, F Nardelli 1, S Manzini 1, C A Maggi 2
PMCID: PMC2362197  PMID: 9888463

Abstract

The neuropeptide substance P (SP), by stimulating tachykinin NK1receptors (NK1R), triggers a number of biological responses in human glioma cells which are potentially relevant for tumour growth. First, radioligand binding studies demonstrated the presence of tachykinin NK1R on SNB-19, DBTRG-05 MG and U373 MG, but not on U138 MG and MOG-G-GCM human glioma cell lines. Second, application of SP or neurokinin A (NKA) to NK1R+glioma cell lines increased the secretion of interleukin 6 (IL-6) and potentiated IL-6 secretion induced by IL-1β. SP also up-regulated the release of transforming growth factor β1 (TGF-β1) by the U373 MG glioma cell line. Third, SP induced new DNA synthesis and enhanced the proliferation rate of NK1R+, but not of NK1R−glioma cell lines. Also, NKA stimulated the proliferation and cytokine secretion in NK1R+glioma cell lines. All the stimulant effects of SP/NKA on NK1R+glioma cell lines were completely blocked by a specific tachykinin NK1R antagonist, MEN 11467. These data support the potential use of tachykinin NK1R antagonist for controlling the proliferative rate of human gliomas. © 1999 Cancer Research Campaign

Keywords: glioma, substance P, tachykinin NK1receptor, cytokine production, proliferation

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Selected References

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