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. 1999 Feb;79(5-6):724–731. doi: 10.1038/sj.bjc.6690116

Genetic aberrations in glioblastoma multiforme: translocation of chromosome 10 in an O-2A-like cell line

X Mao 1,2, T A Jones 2, I Tomlinson 2,3, A J Rowan 2,3, L I Fedorova 2, A V Zelenin 2, J-I Mao 4, N J Gutowski 4,4, M Noble 5,4,5, D Sheer 1
PMCID: PMC2362672  PMID: 10070860

Abstract

We have examined the genetic aberrations in two near-diploid glioblastoma multiforme cell lines that appear to have arisen from different glial lineages. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic of the oligodendrocyte-type-2 astrocyte (0-2A) lineage of the rat central nervous system. This line generates, in vitro, cells with characteristics of 0-2A progenitor cells, oligodendrocytes and astrocytes. The second cell line, IN1434, is derived from an astrocyte or a precursor cell restricted to astrocytic differentiation. In Hu-O-2A/Gb1 the sole homologue of chromosome 10 is disrupted at band 10p11–12.1 by translocation with chromosomes X and 15. The translocation breakpoint is localized between genetic markers D10S2103 and [D10S637, D10S1962, D10S355]. Other aberrations include a 5;14 translocation, deletion of the long and short arms of chromosome 16 and loss of one copy of the CDKN2 gene. IN1434 cells share some cytogenetic abnormalities with Hu-O-2A/Gb1 cells, despite their apparent derivation from a different biological origin, but also have translocations involving the long and short arms of chromosome 1 and the long arm of chromosome 7, and deletion of chromosome 13 at bands 13q12–21. © 1999 Cancer Research Campaign

Keywords: cytogenetics, FISH, molecular genetics, glioblastoma multiforme

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Selected References

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