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. 1999 Apr;80(1-2):279–285. doi: 10.1038/sj.bjc.6690352

The significance of measuring monocyte tissue factor activity in patients with breast and colorectal cancer

B A Lwaleed 1, M Chisholm 1, J L Francis 2
PMCID: PMC2363008  PMID: 10390009

Abstract

Monocytes express tissue factor (mTF) in several conditions including cancer where levels may be valuable in assessing tumour presence and progression. Using a two-stage kinetic chromogenic assay (KCA), mTF levels were measured in controls [normal subjects (n = 60) and patients undergoing hernia repair or cholecystectomy (n = 60)], in patients with benign and malignant disease of the breast (n = 83) and of the large bowel (n = 62). This was performed under fresh (resting) conditions and after incubation for 6 h without (unstimulated) and with (stimulated) Escherichia coli endotoxin. The malignant groups showed higher mTF levels than each of the three controls for resting (P < 0.05 breast, P < 0.05 colorectal) unstimulated (P < 0.05 breast, P < 0.05 colorectal) and stimulated cells (P < 0.001 breast, P < 0.01 colorectal). Similarly, the benign inflammatory groups had higher mTF levels than controls for resting (P < 0.05 colorectal), unstimulated (P < 0.05 colorectal) and stimulated cells (P < 0.01 breast, P < 0.01 colorectal). There was no significant difference between malignant and benign inflammatory groups in each organ. mTF levels showed an increase corresponding to that of histological tumour progression and were higher in non-surviving patients. In conclusion, mTF levels are raised in malignant and inflammatory disease compared to controls and patients with non-inflammatory conditions. Stimulated cells give better discrimination between the groups and may be of value in identifying high risk individuals. mTF levels showed an association with tumour grade or stage and the patients' survival time. © 1999 Cancer Research Campaign

Keywords: monocyte tissue factor, coagulopathy, cancer

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Selected References

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  1. Auger M. J., Mackie M. J. Monocyte procoagulant activity in breast cancer. Thromb Res. 1987 Jul 1;47(1):77–84. doi: 10.1016/0049-3848(87)90242-8. [DOI] [PubMed] [Google Scholar]
  2. Bach R., Nemerson Y., Konigsberg W. Purification and characterization of bovine tissue factor. J Biol Chem. 1981 Aug 25;256(16):8324–8331. [PubMed] [Google Scholar]
  3. Carson S. D., Konigsberg W. H. Cadmium increases tissue factor (coagulation factor III) activity by facilitating its reassociation with lipids. Science. 1980 Apr 18;208(4441):307–309. doi: 10.1126/science.7367861. [DOI] [PubMed] [Google Scholar]
  4. Contrino J., Hair G., Kreutzer D. L., Rickles F. R. In situ detection of tissue factor in vascular endothelial cells: correlation with the malignant phenotype of human breast disease. Nat Med. 1996 Feb;2(2):209–215. doi: 10.1038/nm0296-209. [DOI] [PubMed] [Google Scholar]
  5. Dasmahapatra K. S., Cheung N. K., Spillert C., Lazaro E. An assessment of monocyte procoagulant activity in patients with solid tumors. J Surg Res. 1987 Aug;43(2):158–163. doi: 10.1016/0022-4804(87)90159-4. [DOI] [PubMed] [Google Scholar]
  6. Edwards R. L., Levine J. B., Green R., Duffy M., Mathews E., Brande W., Rickles F. R. Activation of blood coagulation in Crohn's disease. Increased plasma fibrinopeptide A levels and enhanced generation of monocyte tissue factor activity. Gastroenterology. 1987 Feb;92(2):329–337. [PubMed] [Google Scholar]
  7. Edwards R. L., Rickles F. R., Cronlund M. Abnormalities of blood coagulation in patients with cancer. Mononuclear cell tissue factor generation. J Lab Clin Med. 1981 Dec;98(6):917–928. [PubMed] [Google Scholar]
  8. Guha A., Bach R., Konigsberg W., Nemerson Y. Affinity purification of human tissue factor: interaction of factor VII and tissue factor in detergent micelles. Proc Natl Acad Sci U S A. 1986 Jan;83(2):299–302. doi: 10.1073/pnas.83.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hogg N. Human monocytes are associated with the formation of fibrin. J Exp Med. 1983 Feb 1;157(2):473–485. doi: 10.1084/jem.157.2.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lorenzet R., Peri G., Locati D., Allavena P., Colucci M., Semeraro N., Mantovani A., Donati M. B. Generation of procoagulant activity by mononuclear phagocytes: a possible mechanism contributing to blood clotting activation within malignant tissues. Blood. 1983 Aug;62(2):271–273. [PubMed] [Google Scholar]
  11. Lwaleed B. A., Chisholm M., Francis J. L. Urinary tissue factor levels in patients with breast and colorectal cancer. J Pathol. 1999 Feb;187(3):291–294. doi: 10.1002/(SICI)1096-9896(199902)187:3<291::AID-PATH213>3.0.CO;2-8. [DOI] [PubMed] [Google Scholar]
  12. Mellor H., Taylor I., Roath S., Francis J. L. Whole blood procoagulant activity in breast and colorectal cancer. J Clin Pathol. 1989 May;42(5):489–494. doi: 10.1136/jcp.42.5.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morgan D., Edwards R. L., Rickles F. R. Monocyte procoagulant activity as a peripheral marker of clotting activation in cancer patients. Haemostasis. 1988;18(1):55–65. doi: 10.1159/000215783. [DOI] [PubMed] [Google Scholar]
  14. Muller A. D., van Dam-Mieras M. C., Hemker H. C. Measurement of macrophage cellular procoagulant activity. Haemostasis. 1985;15(2):108–113. doi: 10.1159/000215130. [DOI] [PubMed] [Google Scholar]
  15. Naschitz J. E., Yeshurun D., Eldar S., Lev L. M. Diagnosis of cancer-associated vascular disorders. Cancer. 1996 May 1;77(9):1759–1767. doi: 10.1002/(SICI)1097-0142(19960501)77:9<1759::AID-CNCR1>3.0.CO;2-7. [DOI] [PubMed] [Google Scholar]
  16. Nemerson Y., Bach R. Tissue factor revisited. Prog Hemost Thromb. 1982;6:237–261. [PubMed] [Google Scholar]
  17. Nemerson Y., Gentry R. An ordered addition, essential activation model of the tissue factor pathway of coagulation: evidence for a conformational cage. Biochemistry. 1986 Jul 15;25(14):4020–4033. doi: 10.1021/bi00362a006. [DOI] [PubMed] [Google Scholar]
  18. Nemerson Y. The tissue factor pathway of blood coagulation. Semin Hematol. 1992 Jul;29(3):170–176. [PubMed] [Google Scholar]
  19. Osterud B., Bjørklid E. The production and availability of tissue thromboplastin in cellular populations of whole blood exposed to various concentrations of endotoxin. An assay for detection of endotoxin. Scand J Haematol. 1982 Aug;29(2):175–184. doi: 10.1111/j.1600-0609.1982.tb00580.x. [DOI] [PubMed] [Google Scholar]
  20. Osterud B., Due J., Jr Blood coagulation in patients with benign and malignant tumours before and after surgery. Special reference to thromboplastin generation in monocytes. Scand J Haematol. 1984 Mar;32(3):258–264. [PubMed] [Google Scholar]
  21. Osterud B., Flaegstad T. Increased tissue thromboplastin activity in monocytes of patients with meningococcal infection: related to an unfavourable prognosis. Thromb Haemost. 1983 Feb 28;49(1):5–7. [PubMed] [Google Scholar]
  22. Osterud B., Olsen J. O., Wilsgård L. The role of arachidonic acid release and lipoxygenase pathway in lipopolysaccharide-induced thromboplastin activity in monocytes. Blood Coagul Fibrinolysis. 1990 Mar-Apr;1(1):41–46. [PubMed] [Google Scholar]
  23. Parmar J., Taylor I., Roath S., Francis J. Procoagulant activity in whole blood from patients with breast and colorectal cancer. Blood Coagul Fibrinolysis. 1990 Jun;1(2):127–132. [PubMed] [Google Scholar]
  24. Szczepański M., Bardadin K., Zawadzki J., Pypno W. Procoagulant activity of gastric, colorectal, and renal cancer is factor VII-dependent. J Cancer Res Clin Oncol. 1988;114(5):519–522. doi: 10.1007/BF00391504. [DOI] [PubMed] [Google Scholar]

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