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. 1999 Jun;80(7):946–953. doi: 10.1038/sj.bjc.6690448

BPD-MA-mediated photosensitization in vitro and in vivo: cellular adhesion and β1 integrin expression in ovarian cancer cells

J M Runnels 1, N Chen 1, B Ortel 1, D Kato 2, T Hasan 1
PMCID: PMC2363035  PMID: 10362101

Abstract

Benzoporphyrin derivative monoacid (BPD-MA) photosensitization was examined for its effects on cellular adhesion of a human ovarian cancer cell line, OVCAR 3, to extracellular matrix (ECM) components. Mild BPD-MA photosensitization (~ 85% cell survival) of OVCAR 3 transiently decreased adhesion to collagen IV, fibronectin, laminin and vitronectin to a greater extent than could be attributed to cell death. The loss in adhesiveness was accompanied by a loss of β1 integrin-containing focal adhesion plaques (FAPs), although β1 subunits were still recognized by monoclonal antibody directed against human β1 subunits. In vivo BPD-MA photosensitization decreased OVCAR 3 adhesiveness as well. Photosensitized adhesion was reduced in the presence of sodium azide and enhanced in deuterium oxide, suggesting mediation by singlet oxygen. Co-localization studies of BPD-MA and Rhodamine 123 showed that the photosensitizer was largely mitochondrial, but also exhibited extramitochondrial, intracellullar, diffuse cytosolic fluorescence. Taken together, these data show that intracellular damage mediated by BPD-PDT remote from the FAP site can affect cellular–ECM interactions and result in loss of FAP formation. This may have an impact on long-term effects of photodynamic therapy. The topic merits further investigation. © 1999 Cancer Research Campaign

Keywords: BPD-MA, photosensitization, cellular adhesion, ECM proteins, OVCAR 3, ovarian cancer

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Selected References

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