Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1999 Jul;80(10):1571–1574. doi: 10.1038/sj.bjc.6690563

Lack of Bcl10 mutations in testicular germ cell tumours and derived cell lines

E M van Schothorst 1,2, S Mohkamsing 1, R J H L M van Gurp 1, J W Oosterhuis 1, P T van der Saag 2, L H J Looijenga 1
PMCID: PMC2363097  PMID: 10408400

Abstract

Aberrations within Bcl10, a gene involved in execution of apoptosis, has most recently been found in a variety of cancers, including cell lines of testicular germ cell tumours of adolescents and adults (TGCTs). To study this in more detail, we screened exons 2 and 3 of this gene for mutations in a larger series of cell lines as well as primary TGCTs by single-strand conformation polymorphism and endonuclease restriction analysis. Because no aberrations were detected, we conclude that inactivation of Bcl10 by mutation is at least far less important in the development of TGCTs than proposed. © 1999 Cancer Research Campaign

Keywords: testicular cancer, Bcl10, mutation-analysis

Full Text

The Full Text of this article is available as a PDF (149.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. W., Casper J., Damjanov I., Duggan-Keen M., Giwercman A., Hata J., von Keitz A., Looijenga L. H., Millán J. L., Oosterhuis J. W. Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours. Int J Cancer. 1996 Jun 11;66(6):806–816. doi: 10.1002/(SICI)1097-0215(19960611)66:6<806::AID-IJC17>3.0.CO;2-0. [DOI] [PubMed] [Google Scholar]
  2. Hussong J., Crussi F. G., Chou P. M. Gonadoblastoma: immunohistochemical localization of Müllerian-inhibiting substance, inhibin, WT-1, and p53. Mod Pathol. 1997 Nov;10(11):1101–1105. [PubMed] [Google Scholar]
  3. Jørgensen N., Müller J., Jaubert F., Clausen O. P., Skakkebaek N. E. Heterogeneity of gonadoblastoma germ cells: similarities with immature germ cells, spermatogonia and testicular carcinoma in situ cells. Histopathology. 1997 Feb;30(2):177–186. doi: 10.1046/j.1365-2559.1997.d01-580.x. [DOI] [PubMed] [Google Scholar]
  4. Landegren U., Nilsson M., Kwok P. Y. Reading bits of genetic information: methods for single-nucleotide polymorphism analysis. Genome Res. 1998 Aug;8(8):769–776. doi: 10.1101/gr.8.8.769. [DOI] [PubMed] [Google Scholar]
  5. Looijenga L. H., Gillis A. J., van Gurp R. J., Verkerk A. J., Oosterhuis J. W. X inactivation in human testicular tumors. XIST expression and androgen receptor methylation status. Am J Pathol. 1997 Aug;151(2):581–590. [PMC free article] [PubMed] [Google Scholar]
  6. Looijenga L. H., Oosterhuis J. W. Pathogenesis of testicular germ cell tumours. Rev Reprod. 1999 May;4(2):90–100. doi: 10.1530/ror.0.0040090. [DOI] [PubMed] [Google Scholar]
  7. Mathew S., Murty V. V., Bosl G. J., Chaganti R. S. Loss of heterozygosity identifies multiple sites of allelic deletions on chromosome 1 in human male germ cell tumors. Cancer Res. 1994 Dec 1;54(23):6265–6269. [PubMed] [Google Scholar]
  8. Oosterhuis J. W., Castedo S. M., de Jong B., Cornelisse C. J., Dam A., Sleijfer D. T., Schraffordt Koops H. Ploidy of primary germ cell tumors of the testis. Pathogenetic and clinical relevance. Lab Invest. 1989 Jan;60(1):14–21. [PubMed] [Google Scholar]
  9. Rosenberg C., Mostert M. C., Schut T. B., van de Pol M., van Echten J., de Jong B., Raap A. K., Tanke H., Oosterhuis J. W., Looijenga L. H. Chromosomal constitution of human spermatocytic seminomas: comparative genomic hybridization supported by conventional and interphase cytogenetics. Genes Chromosomes Cancer. 1998 Dec;23(4):286–291. [PubMed] [Google Scholar]
  10. Salvesen G. S., Dixit V. M. Caspases: intracellular signaling by proteolysis. Cell. 1997 Nov 14;91(4):443–446. doi: 10.1016/s0092-8674(00)80430-4. [DOI] [PubMed] [Google Scholar]
  11. Sandberg A. A., Meloni A. M., Suijkerbuijk R. F. Reviews of chromosome studies in urological tumors. III. Cytogenetics and genes in testicular tumors. J Urol. 1996 May;155(5):1531–1556. [PubMed] [Google Scholar]
  12. Sinke R. J., Weghuis D. O., Suijkerbuijk R. F., Tanigami A., Nakamura Y., Larsson C., Weber G., de Jong B., Oosterhuis J. W., Molenaar W. M. Molecular characterization of a recurring complex chromosomal translocation in two human extragonadal germ cell tumors. Cancer Genet Cytogenet. 1994 Mar;73(1):11–16. doi: 10.1016/0165-4608(94)90176-7. [DOI] [PubMed] [Google Scholar]
  13. Skakkebaek N. E. Possible carcinoma-in-situ of the testis. Lancet. 1972 Sep 9;2(7776):516–517. doi: 10.1016/s0140-6736(72)91909-5. [DOI] [PubMed] [Google Scholar]
  14. Tsujimoto Y., Yunis J., Onorato-Showe L., Erikson J., Nowell P. C., Croce C. M. Molecular cloning of the chromosomal breakpoint of B-cell lymphomas and leukemias with the t(11;14) chromosome translocation. Science. 1984 Jun 29;224(4656):1403–1406. doi: 10.1126/science.6610211. [DOI] [PubMed] [Google Scholar]
  15. Ulbright T. M. Germ cell neoplasms of the testis. Am J Surg Pathol. 1993 Nov;17(11):1075–1091. doi: 10.1097/00000478-199311000-00001. [DOI] [PubMed] [Google Scholar]
  16. Versnel M. A., Hoogsteden H. C., Hagemeijer A., Bouts M. J., van der Kwast T. H., Delahaye M., Schaart G., Ramaekers F. C. Characterization of three human malignant mesothelioma cell lines. Cancer Genet Cytogenet. 1989 Oct 1;42(1):115–128. doi: 10.1016/0165-4608(89)90014-9. [DOI] [PubMed] [Google Scholar]
  17. Willis T. G., Jadayel D. M., Du M. Q., Peng H., Perry A. R., Abdul-Rauf M., Price H., Karran L., Majekodunmi O., Wlodarska I. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell. 1999 Jan 8;96(1):35–45. doi: 10.1016/s0092-8674(00)80957-5. [DOI] [PubMed] [Google Scholar]
  18. de Graaff W. E., Oosterhuis J. W., de Jong B., Dam A., van Putten W. L., Castedo S. M., Sleijfer D. T., Schraffordt Koops H. Ploidy of testicular carcinoma in situ. Lab Invest. 1992 Feb;66(2):166–168. [PubMed] [Google Scholar]
  19. van Echten J., Oosterhuis J. W., Looijenga L. H., van de Pol M., Wiersema J., te Meerman G. J., Schaffordt Koops H., Sleijfer D. T., de Jong B. No recurrent structural abnormalities apart from i(12p) in primary germ cell tumors of the adult testis. Genes Chromosomes Cancer. 1995 Oct;14(2):133–144. doi: 10.1002/gcc.2870140208. [DOI] [PubMed] [Google Scholar]
  20. von Keitz A. T., Riedmiller H., Neumann K., Gutschank W., Fonatsch C. Establishment and characterization of a seminoma cell-line (S2). Investig Urol (Berl) 1994;5:28–31. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES