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. 1994 Jan;68(1):566–569. doi: 10.1128/jvi.68.1.566-569.1994

Screening retroviral packaging cells for highly efficient virus production by using a combined selection procedure.

M Wirth 1, R Grannemann 1, D Klehr 1, H Hauser 1
PMCID: PMC236323  PMID: 8254773

Abstract

To facilitate the screening for clones of transfected packaging cells producing a high yield of recombinant retrovirus, we present a fast and simple method for the isolation of overexpressing cells. By this method the efficiency of virus production can generally be enhanced 10- to 100-fold by application of high selection pressure. Cell lines which exhibit titers of up to 10(8) CFU/ml were obtained.

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Selected References

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