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. 2000 Jan 18;82(2):492–496. doi: 10.1054/bjoc.1999.0948

Modulation of oestrone sulphate formation and hydrolysis in breast cancer cells by breast cyst fluid from British and Hungarian women

A Purohit 1, B Budai 2, D Y Wang 1, E L Willemsen 1, A de Winkel 1, D Parish 1, M W Ghilchik 3, I Szamel 2, M J Reed 1
PMCID: PMC2363274  PMID: 10646910

Abstract

Women with gross cystic breast disease may have an increased risk of breast cancer. In this study the ability of breast cyst fluid (BCF), obtained from British or Hungarian women, to modulate oestrone sulphate (E1S) formation or hydrolysis, has been examined. For this, oestrogen receptor-positive (ER+) MCF-7 or MDA-MB-231 (ER–) breast cancer cells were employed. The formation and hydrolysis of E1S was measured using radiometric techniques. BCF from British and Hungarian women mainly inhibited E1S hydrolysis in MCF-7 cells while stimulating hydrolysis in MDA-MB-231 cells. The extent of inhibition or stimulation of E1S hydrolysis in these cells was related to the Na+/K+ratio of the BCF. There was a significant inverse relationship between the extent to which BCF samples inhibited hydrolysis in MCF-7 cells and stimulated it in MDA-MB-231 cells. BCF stimulated E1S formation in MCF-7 cells while inhibiting formation in MDA-MB-231 cells. No difference in the ability of BCF from British or Hungarian women to inhibit or stimulate E1S hydrolysis was detected in ER+ or ER– breast cancer cells. In contrast, BCF from British women stimulated E1S formation in ER+ cells (median 82%) to a significantly greater extent (P< 0.01) than BCF from Hungarian women (median 33%). The role that E1S has in breast cancer development remains unclear. The greater stimulation of E1S formation by BCF from British women, who have a higher risk of breast cancer than Hungarian women, suggests that it may act as a storage form of oestrogen within cells that can be activated by oestrone sulphatase. © 2000 Cancer Research Campaign

Keywords: breast cysts, breast cyst fluid, oestrogens, oestrone sulphate, oestrone sulphatase

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Selected References

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  1. Armstrong B., Doll R. Environmental factors and cancer incidence and mortality in different countries, with special reference to dietary practices. Int J Cancer. 1975 Apr 15;15(4):617–631. doi: 10.1002/ijc.2910150411. [DOI] [PubMed] [Google Scholar]
  2. Bruzzi P., Dogliotti L., Naldoni C., Bucchi L., Costantini M., Cicognani A., Torta M., Buzzi G. F., Angeli A. Cohort study of association of risk of breast cancer with cyst type in women with gross cystic disease of the breast. BMJ. 1997 Mar 29;314(7085):925–928. doi: 10.1136/bmj.314.7085.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dixon J. M., Lumsden A. B., Miller W. R. The relationship of cyst type to risk factors for breast cancer and the subsequent development of breast cancer in patients with breast cystic disease. Eur J Cancer Clin Oncol. 1985 Sep;21(9):1047–1050. doi: 10.1016/0277-5379(85)90289-5. [DOI] [PubMed] [Google Scholar]
  4. Dixon J. M., McDonald C., Elton R. A., Miller W. R. Breast cancer risk with cyst type in cystic disease of the breast. Larger study found no association between cyst type and breast cancer. BMJ. 1997 Aug 30;315(7107):545–546. [PMC free article] [PubMed] [Google Scholar]
  5. Dixon J. M., McDonald C., Elton R. A., Miller W. R. Risk of breast cancer in women with palpable breast cysts: a prospective study. Edinburgh Breast Group. Lancet. 1999 May 22;353(9166):1742–1745. doi: 10.1016/s0140-6736(98)06408-3. [DOI] [PubMed] [Google Scholar]
  6. Duncan L. J., Coldham N. G., Reed M. J. The interaction of cytokines in regulating oestradiol 17 beta-hydroxysteroid dehydrogenase activity in MCF-7 cells. J Steroid Biochem Mol Biol. 1994 May;49(1):63–68. doi: 10.1016/0960-0760(94)90301-8. [DOI] [PubMed] [Google Scholar]
  7. Erbas H., Lennard T. W., Lai L. C. Effect of breast cyst fluid on oestrone sulphatase activity in MCF-7 breast cancer cells. Anticancer Res. 1996 Mar-Apr;16(2):833–836. [PubMed] [Google Scholar]
  8. Falany J. L., Lawing L., Falany C. N. Identification and characterization of cytosolic sulfotransferase activities in MCF-7 human breast carcinoma cells. J Steroid Biochem Mol Biol. 1993 Oct;46(4):481–487. doi: 10.1016/0960-0760(93)90102-3. [DOI] [PubMed] [Google Scholar]
  9. Lai L. C., Coldham N. G., Islam S., Reed M. J., Ghilchik M. W., James V. H. Effect of breast cyst fluid on oestrogen 17-oxidoreductase activity in MCF-7 breast cancer cells. Cancer Lett. 1990 Dec 3;55(2):165–169. doi: 10.1016/0304-3835(90)90028-v. [DOI] [PubMed] [Google Scholar]
  10. Lai L. C., Dunkley S. A., Reed M. J., Ghilchik M. W., Shaikh N. A., James V. H. Epidermal growth factor and oestradiol in human breast cyst fluid. Eur J Cancer. 1990 Apr;26(4):481–484. doi: 10.1016/0277-5379(90)90021-k. [DOI] [PubMed] [Google Scholar]
  11. Miller W. R., Dixon J. M., Scott W. N., Forrest A. P. Classification of human breast cysts according to electrolyte and androgen conjugate composition. Clin Oncol. 1983 Sep;9(3):227–232. [PubMed] [Google Scholar]
  12. Pasqualini J. R., Gelly C., Nguyen B. L., Vella C. Importance of estrogen sulfates in breast cancer. J Steroid Biochem. 1989;34(1-6):155–163. doi: 10.1016/0022-4731(89)90077-0. [DOI] [PubMed] [Google Scholar]
  13. Purohit A., Reed M. J., Morris N. C., Williams G. J., Potter B. V. Regulation and inhibition of steroid sulfatase activity in breast cancer. Ann N Y Acad Sci. 1996 Apr 30;784:40–49. doi: 10.1111/j.1749-6632.1996.tb16226.x. [DOI] [PubMed] [Google Scholar]
  14. Purohit A., Reed M. J. Oestrogen sulphatase activity in hormone-dependent and hormone-independent breast-cancer cells: modulation by steroidal and non-steroidal therapeutic agents. Int J Cancer. 1992 Apr 1;50(6):901–905. doi: 10.1002/ijc.2910500614. [DOI] [PubMed] [Google Scholar]
  15. Purohit A., de Giovani C. V., Reed M. J. The regulation of oestrone sulphate formation in breast cancer cells. J Steroid Biochem Mol Biol. 1999 Feb;68(3-4):129–135. doi: 10.1016/s0960-0760(99)00023-0. [DOI] [PubMed] [Google Scholar]
  16. Reed M. J., Topping L., Coldham N. G., Purohit A., Ghilchik M. W., James V. H. Control of aromatase activity in breast cancer cells: the role of cytokines and growth factors. J Steroid Biochem Mol Biol. 1993 Mar;44(4-6):589–596. doi: 10.1016/0960-0760(93)90264-w. [DOI] [PubMed] [Google Scholar]
  17. Sánchez L. M., Vizoso F., Díez-Itza I., López-Otín C. Identification of the major protein components in breast secretions from women with benign and malignant breast diseases. Cancer Res. 1992 Jan 1;52(1):95–100. [PubMed] [Google Scholar]
  18. Wild M. J., Rudland P. S., Back D. J. Metabolism of the oral contraceptive steroids ethynylestradiol and norgestimate by normal (Huma 7) and malignant (MCF-7 and ZR-75-1) human breast cells in culture. J Steroid Biochem Mol Biol. 1991 Oct;39(4A):535–543. doi: 10.1016/0960-0760(91)90248-4. [DOI] [PubMed] [Google Scholar]

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