Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 2000 Apr 3;82(9):1510–1512. doi: 10.1054/bjoc.1999.1170

Human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50 are admixtures of the human colon carcinoma cell line HCT 116

B P L Wijnhoven 1,2, M G J Tilanus 3, A G Morris 4, S J Darnton 5, H W Tilanus 1,2, W N M Dinjens 1,6
PMCID: PMC2363398  PMID: 10789716

Abstract

In two recently described human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50, derived from one tumour, we detected identical E-cadherin and β-catenin gene mutations as in colon carcinoma cell line HCT 116. We demonstrate by HLA-typing, mutation analysis and microsatellite analysis that cell lines JROECL 47 and JROECL 50 are admixtures of the human colon adenocarcinoma cell line HCT 116. © 2000 Cancer Research Campaign

Keywords: adenocarcinoma, oesophagus, colon, cell lines, admixtures

Full Text

The Full Text of this article is available as a PDF (103.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brattain M. G., Fine W. D., Khaled F. M., Thompson J., Brattain D. E. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 1981 May;41(5):1751–1756. [PubMed] [Google Scholar]
  2. Buard A., Zipfel P. A., Frey R. S., Mulder K. M. Maintenance of growth factor signaling through Ras in human colon carcinoma cells containing K-ras mutations. Int J Cancer. 1996 Aug 7;67(4):539–546. doi: 10.1002/(SICI)1097-0215(19960807)67:4<539::AID-IJC13>3.0.CO;2-2. [DOI] [PubMed] [Google Scholar]
  3. Efstathiou J. A., Liu D., Wheeler J. M., Kim H. C., Beck N. E., Ilyas M., Karayiannakis A. J., Mortensen N. J., Kmiot W., Playford R. J. Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2316–2321. doi: 10.1073/pnas.96.5.2316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fukuchi T., Sakamoto M., Tsuda H., Maruyama K., Nozawa S., Hirohashi S. Beta-catenin mutation in carcinoma of the uterine endometrium. Cancer Res. 1998 Aug 15;58(16):3526–3528. [PubMed] [Google Scholar]
  5. Grady W. M., Rajput A., Myeroff L., Liu D. F., Kwon K., Willis J., Markowitz S. Mutation of the type II transforming growth factor-beta receptor is coincident with the transformation of human colon adenomas to malignant carcinomas. Cancer Res. 1998 Jul 15;58(14):3101–3104. [PubMed] [Google Scholar]
  6. Hoang J. M., Cottu P. H., Thuille B., Salmon R. J., Thomas G., Hamelin R. BAT-26, an indicator of the replication error phenotype in colorectal cancers and cell lines. Cancer Res. 1997 Jan 15;57(2):300–303. [PubMed] [Google Scholar]
  7. Ilyas M., Tomlinson I. P., Rowan A., Pignatelli M., Bodmer W. F. Beta-catenin mutations in cell lines established from human colorectal cancers. Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10330–10334. doi: 10.1073/pnas.94.19.10330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lane J. R. Clinical Lecture on Injuries from Lightning. Br Med J. 1872 Aug 3;2(605):114–116. doi: 10.1136/bmj.2.605.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McGinnis M. D., Conrad M. P., Bouwens A. G., Tilanus M. G., Kronick M. N. Automated, solid-phase sequencing of DRB region genes using T7 sequencing chemistry and dye-labeled primers. Tissue Antigens. 1995 Sep;46(3 ):173–179. doi: 10.1111/j.1399-0039.1995.tb03116.x. [DOI] [PubMed] [Google Scholar]
  10. McKinney A., Sharp L., Macfarlane G. J., Muir C. S. Oesophageal and gastric cancer in Scotland 1960-90. Br J Cancer. 1995 Feb;71(2):411–415. doi: 10.1038/bjc.1995.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oki E., Oda S., Maehara Y., Sugimachi K. Mutated gene-specific phenotypes of dinucleotide repeat instability in human colorectal carcinoma cell lines deficient in DNA mismatch repair. Oncogene. 1999 Mar 25;18(12):2143–2147. doi: 10.1038/sj.onc.1202583. [DOI] [PubMed] [Google Scholar]
  12. Rockett J. C., Larkin K., Darnton S. J., Morris A. G., Matthews H. R. Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization. Br J Cancer. 1997;75(2):258–263. doi: 10.1038/bjc.1997.42. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schipper R. F., Schreuder G. M., D'Amaro J., Oudshoorn M. HLA gene and haplotype frequencies in Dutch blood donors. Tissue Antigens. 1996 Nov;48(5):562–574. doi: 10.1111/j.1399-0039.1996.tb02670.x. [DOI] [PubMed] [Google Scholar]
  14. Trapman J., Sleddens H. F., van der Weiden M. M., Dinjens W. N., Konig J. J., Schroder F. H., Faber P. W., Bosman F. T. Loss of heterozygosity of chromosome 8 microsatellite loci implicates a candidate tumor suppressor gene between the loci D8S87 and D8S133 in human prostate cancer. Cancer Res. 1994 Dec 1;54(23):6061–6064. [PubMed] [Google Scholar]
  15. Versluis L. F., Rozemuller E., Tonks S., Marsh S. G., Bouwens A. G., Bodmer J. G., Tilanus M. G. High-resolution HLA-DPB typing based upon computerized analysis of data obtained by fluorescent sequencing of the amplified polymorphic exon 2. Hum Immunol. 1993 Dec;38(4):277–283. doi: 10.1016/0198-8859(93)90555-f. [DOI] [PubMed] [Google Scholar]
  16. Wijnhoven B. P., de Both N. J., van Dekken H., Tilanus H. W., Dinjens W. N. E-cadherin gene mutations are rare in adenocarcinomas of the oesophagus. Br J Cancer. 1999 Jul;80(10):1652–1657. doi: 10.1038/sj.bjc.6690577. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES