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. 2000 Jun 15;83(2):215–218. doi: 10.1054/bjoc.2000.1195

Immunohistochemical detection of membrane-type-1-matrix metalloproteinase in colorectal carcinoma

R Kikuchi 1, T Noguchi 1, S Takeno 1, N Kubo 1, Y Uchida 1
PMCID: PMC2363481  PMID: 10901373

Abstract

We investigated whether the expression of membrane-type-1 matrix metalloproteinase (MT1-MMP), matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) was consistent with the proposed roles of these proteins in promoting metastasis in colorectal cancer. The expression of MT1-MMP was significantly more frequent in deeply invasive carcinomas (P = 0.007) and in cases of vascular invasion (P = 0.02). The frequency of detection of MMP-2 in the stroma was much greater in vascular invasion-positive cases (42%) than in negative cases (20%;P = 0.02). The rate of detection of TIMP-2 in tumour cell cytoplasm increased with the depth of invasion (P = 0.03). TIMP-2 in the stroma was found more frequently in tumours with lymphatic invasion and lymph node metastasis (P< 0.05). Significant correlations were found between detection of MT1-MMP and MMP-2 in tumour cell cytoplasm (P< 0.05), of MT1-MMP and TIMP-2 in tumour cell cytoplasm (P< 0.01), and of MMP-2 and TIMP-2 in tumour cell cytoplasm (P< 0.01). Immunohistochemical detection of MT1-MMP and TIMP-2 might be useful for monitoring infiltration in colorectal carcinoma but is not correlated with distant metastases. © 2000 Cancer Research Campaign

Keywords: membrane-type-1-matrix metalloproteinase, matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-2, immunohistochemistry, distant metastases, colorectal carcinoma

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Selected References

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