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. 2001 Jul;85(1):102–106. doi: 10.1054/bjoc.2001.1904

Quantitative measurement of thyroglobulin mRNA in peripheral blood of patients after total thyroidectomy

T Takano 1, A Miyauchi 2, H Yoshida 2, Y Hasegawa 1, K Kuma 2, N Amino 1
PMCID: PMC2363919  PMID: 11437410

Abstract

Previous studies have reported the clinical usefulness of reverse transcription-polymerase chain reaction (RT-PCR) detection of thyroglobulin (TG) mRNA in the peripheral blood of patients with differentiated thyroid carcinoma. To evaluate this usefulness, we measured TG mRNA in the peripheral blood of patients diagnosed with thyroid carcinoma after total thyroidectomy by real-time quantitative RT-PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control. Surprisingly, we detected TG mRNA in all samples obtained after total thyroidectomy, including those from 4 medullary carcinomas. Further, there was no statistical difference in expression levels of TG mRNA in the patients with or without metastasis, and no significant correlation was found between serum TG concentrations and the expression levels of TG mRNA. These results give rise to a question regarding the clinical applications of not only RT-PCR detection but also quantitative measurement of TG mRNA in peripheral blood. © 2001 Cancer Research Campaign http://www.bjcancer.com

Keywords: thyroglobulin, real-time quantitative RT-PCR, thyroid cancer, molecular-based diagnosis

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Selected References

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  1. Bojunga J., Röddiger S., Stanisch M., Kusterer K., Kurek R., Renneberg H., Adams S., Lindhorst E., Usadel K. H., Schumm-Draeger P. M. Molecular detection of thyroglobulin mRNA transcripts in peripheral blood of patients with thyroid disease by RT-PCR. Br J Cancer. 2000 May;82(10):1650–1655. doi: 10.1054/bjoc.1999.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  3. Coutelier J. P., Godfraind C., Dveksler G. S., Wysocka M., Cardellichio C. B., Noël H., Holmes K. V. B lymphocyte and macrophage expression of carcinoembryonic antigen-related adhesion molecules that serve as receptors for murine coronavirus. Eur J Immunol. 1994 Jun;24(6):1383–1390. doi: 10.1002/eji.1830240622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Endo T., Ohta K., Haraguchi K., Onaya T. Cloning and functional expression of a thyrotropin receptor cDNA from rat fat cells. J Biol Chem. 1995 May 5;270(18):10833–10837. doi: 10.1074/jbc.270.18.10833. [DOI] [PubMed] [Google Scholar]
  5. Francis T., Burch H. B., Cai W. Y., Lukes Y., Peele M., Carr F. E., Wartofsky L., Burman K. D. Lymphocytes express thyrotropin receptor-specific mRNA as detected by the PCR technique. Thyroid. 1991 Summer;1(3):223–228. doi: 10.1089/thy.1991.1.223. [DOI] [PubMed] [Google Scholar]
  6. Ghossein R. A., Scher H. I., Gerald W. L., Kelly W. K., Curley T., Amsterdam A., Zhang Z. F., Rosai J. Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: clinical implications. J Clin Oncol. 1995 May;13(5):1195–1200. doi: 10.1200/JCO.1995.13.5.1195. [DOI] [PubMed] [Google Scholar]
  7. Lafarge-Frayssinet C., Torres J. M., Frain M., Uriel J. Alpha-fetoprotein gene expression in human lymphoblastoid cells and in PHA-stimulated normal T-lymphocytes. Biochem Biophys Res Commun. 1989 Feb 28;159(1):112–118. doi: 10.1016/0006-291x(89)92411-x. [DOI] [PubMed] [Google Scholar]
  8. Loh K. C., Greenspan F. S., Gee L., Miller T. R., Yeo P. P. Pathological tumor-node-metastasis (pTNM) staging for papillary and follicular thyroid carcinomas: a retrospective analysis of 700 patients. J Clin Endocrinol Metab. 1997 Nov;82(11):3553–3562. doi: 10.1210/jcem.82.11.4373. [DOI] [PubMed] [Google Scholar]
  9. Mattano L. A., Jr, Moss T. J., Emerson S. G. Sensitive detection of rare circulating neuroblastoma cells by the reverse transcriptase-polymerase chain reaction. Cancer Res. 1992 Sep 1;52(17):4701–4705. [PubMed] [Google Scholar]
  10. Ringel M. D., Balducci-Silano P. L., Anderson J. S., Spencer C. A., Silverman J., Sparling Y. H., Francis G. L., Burman K. D., Wartofsky L., Ladenson P. W. Quantitative reverse transcription-polymerase chain reaction of circulating thyroglobulin messenger ribonucleic acid for monitoring patients with thyroid carcinoma. J Clin Endocrinol Metab. 1999 Nov;84(11):4037–4042. doi: 10.1210/jcem.84.11.6164. [DOI] [PubMed] [Google Scholar]
  11. Ringel M. D., Ladenson P. W., Levine M. A. Molecular diagnosis of residual and recurrent thyroid cancer by amplification of thyroglobulin messenger ribonucleic acid in peripheral blood. J Clin Endocrinol Metab. 1998 Dec;83(12):4435–4442. doi: 10.1210/jcem.83.12.5346. [DOI] [PubMed] [Google Scholar]
  12. Singer P. A., Cooper D. S., Daniels G. H., Ladenson P. W., Greenspan F. S., Levy E. G., Braverman L. E., Clark O. H., McDougall I. R., Ain K. V. Treatment guidelines for patients with thyroid nodules and well-differentiated thyroid cancer. American Thyroid Association. Arch Intern Med. 1996 Oct 28;156(19):2165–2172. [PubMed] [Google Scholar]
  13. Takano T., Miyauchi A., Yokozawa T., Matsuzuka F., Maeda I., Kuma K., Amino N. Preoperative diagnosis of thyroid papillary and anaplastic carcinomas by real-time quantitative reverse transcription-polymerase chain reaction of oncofetal fibronectin messenger RNA. Cancer Res. 1999 Sep 15;59(18):4542–4545. [PubMed] [Google Scholar]
  14. Tallini G., Ghossein R. A., Emanuel J., Gill J., Kinder B., Dimich A. B., Costa J., Robbins R., Burrow G. N., Rosai J. Detection of thyroglobulin, thyroid peroxidase, and RET/PTC1 mRNA transcripts in the peripheral blood of patients with thyroid disease. J Clin Oncol. 1998 Mar;16(3):1158–1166. doi: 10.1200/JCO.1998.16.3.1158. [DOI] [PubMed] [Google Scholar]
  15. Wingo S. T., Ringel M. D., Anderson J. S., Patel A. D., Lukes Y. D., Djuh Y. Y., Solomon B., Nicholson D., Balducci-Silano P. L., Levine M. A. Quantitative reverse transcription-PCR measurement of thyroglobulin mRNA in peripheral blood of healthy subjects. Clin Chem. 1999 Jun;45(6 Pt 1):785–789. [PubMed] [Google Scholar]

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