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. 2001 Nov;85(11):1787–1793. doi: 10.1054/bjoc.2001.2170

Internalisation enhances photo-induced cytotoxicity of monoclonal antibody-phthalocyanine conjugates

M Carcenac 1, M Dorvillius 1, V Garambois 1, F Glaussel 1, Ch Larroque 2, R Langlois 3, N E Hynes 4, J E van Lier 3, A Pèlegrin 1
PMCID: PMC2363963  PMID: 11742503

Abstract

Immunophototherapy of cancer combines the specificity of a monoclonal antibody (MAb) to an overexpressed tumor marker with the phototoxic properties of the conjugated dye. To analyze the potential role of internalisation of the dye on photo-induced cytotoxicity, we compared two target antigens, carcinoembryonic antigen (CEA) that does not internalise and ErbB2 that does. Human ovarian carcinoma SKOv3 cells that express a high level of ErbB2 were transfected with the CEA cDNA. Using FACS analysis, the resulting cell line, SKOv3-CEA-1B9, demonstrated comparable levels of expression of the two target antigens. Aluminium tetrasulfophthalocyanine (AlPcS 4) was covalently coupled to anti-CEA MAb 35A7, anti-ErbB2 MAb FSP77 and a non-specific MAb PX, via a five-carbon sulfonamide spacer chain (A 1) at molar ratios ranging from 6 to 9 moles of AlPcS 4 per mole of MAb. The 35A7-(AlPcS 4 A 1)8 conjugate induced 68% growth inhibition of the SKOv3-CEA-1B9 cell line after a 20 h incubation at 2.50 μg/ml (based on AlPcS 4 A 1 content) following light exposure. However, the FSP77-(AlPcS 4 A 1)6 conjugate gave a 51% growth inhibition for an AlPcS 4 A 1 concentration as low as 0.04 μg/ml after the same incubation time and exposure to the same light dose. At a 1.25 μg/ml AlPcS 4 A 1 concentration, the FSP77-(AlPcS 4 A 1)6 conjugate gave a 67% growth inhibition after an incubation time as short as 1 h, reaching a 96% inhibition after an 8 h incubation time. Using an unique cell line that expresses two different target antigens, we demonstrated a clear advantage of an internalising over a non-internalising MAb-dye conjugate in terms of phototoxic efficacy. In vivo evaluation of the photodynamic properties of the conjugates is in progress. © 2001 Cancer Research Campaign http://www.bjcancer.com © 2001 Cancer Research Campaign

Keywords: immunophototherapy, phthalocyanine, monoclonal antibody, CEA, ErbB2

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Selected References

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