Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1994 Jul;68(7):4673–4678. doi: 10.1128/jvi.68.7.4673-4678.1994

A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases.

A L Passarelli 1, J W Todd 1, L K Miller 1
PMCID: PMC236397  PMID: 8207843

Abstract

We have identified and sequenced a novel baculovirus gene, late expression factor eight gene (lef-8), of Autographa californica nuclear polyhedrosis virus that is necessary for efficient expression from late and very late virus gene promoters in a transient expression assay. The predicted gene product, LEF-8, has a molecular mass of 102 kDa and contains a conserved sequence motif, GXKX4HGQ/NKG, found in DNA-directed RNA polymerases throughout the animal, plant, and microbial kingdoms.

Full text

PDF
4673

Images in this article

4674Image
on p.4674
4675Image
on p.4675

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amegadzie B. Y., Holmes M. H., Cole N. B., Jones E. V., Earl P. L., Moss B. Identification, sequence, and expression of the gene encoding the second-largest subunit of the vaccinia virus DNA-dependent RNA polymerase. Virology. 1991 Jan;180(1):88–98. doi: 10.1016/0042-6822(91)90012-z. [DOI] [PubMed] [Google Scholar]
  2. Chen Y., Weeks J., Mortin M. A., Greenleaf A. L. Mapping mutations in genes encoding the two large subunits of Drosophila RNA polymerase II defines domains essential for basic transcription functions and for proper expression of developmental genes. Mol Cell Biol. 1993 Jul;13(7):4214–4222. doi: 10.1128/mcb.13.7.4214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crawford A. M., Miller L. K. Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1988 Aug;62(8):2773–2781. doi: 10.1128/jvi.62.8.2773-2781.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuchs L. Y., Woods M. S., Weaver R. F. Viral Transcription During Autographa californica Nuclear Polyhedrosis Virus Infection: a Novel RNA Polymerase Induced in Infected Spodoptera frugiperda Cells. J Virol. 1983 Dec;48(3):641–646. doi: 10.1128/jvi.48.3.641-646.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glocker B., Hoopes R. R., Jr, Hodges L., Rohrmann G. F. In vitro transcription from baculovirus late gene promoters: accurate mRNA initiation by nuclear extracts prepared from infected Spodoptera frugiperda cells. J Virol. 1993 Jul;67(7):3771–3776. doi: 10.1128/jvi.67.7.3771-3776.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grula M. A., Buller P. L., Weaver R. F. alpha-Amanitin-Resistant Viral RNA Synthesis in Nuclei Isolated from Nuclear Polyhedrosis Virus-Infected Heliothis zea Larvae and Spodoptera frugiperda Cells. J Virol. 1981 Jun;38(3):916–921. doi: 10.1128/jvi.38.3.916-921.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoopes R. R., Jr, Rohrmann G. F. In vitro transcription of baculovirus immediate early genes: accurate mRNA initiation by nuclear extracts from both insect and human cells. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4513–4517. doi: 10.1073/pnas.88.10.4513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huh N. E., Weaver R. F. Categorizing some early and late transcripts directed by the Autographa californica nuclear polyhedrosis virus. J Gen Virol. 1990 Sep;71(Pt 9):2195–2200. doi: 10.1099/0022-1317-71-9-2195. [DOI] [PubMed] [Google Scholar]
  9. Huh N. E., Weaver R. F. Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus. J Gen Virol. 1990 Jan;71(Pt 1):195–201. doi: 10.1099/0022-1317-71-1-195. [DOI] [PubMed] [Google Scholar]
  10. Li Y., Passarelli A. L., Miller L. K. Identification, sequence, and transcriptional mapping of lef-3, a baculovirus gene involved in late and very late gene expression. J Virol. 1993 Sep;67(9):5260–5268. doi: 10.1128/jvi.67.9.5260-5268.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mustaev A., Kashlev M., Lee J. Y., Polyakov A., Lebedev A., Zalenskaya K., Grachev M., Goldfarb A., Nikiforov V. Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase. J Biol Chem. 1991 Dec 15;266(35):23927–23931. [PubMed] [Google Scholar]
  12. Passarelli A. L., Miller L. K. Identification and characterization of lef-1, a baculovirus gene involved in late and very late gene expression. J Virol. 1993 Jun;67(6):3481–3488. doi: 10.1128/jvi.67.6.3481-3488.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Passarelli A. L., Miller L. K. Identification and transcriptional regulation of the baculovirus lef-6 gene. J Virol. 1994 Jul;68(7):4458–4467. doi: 10.1128/jvi.68.7.4458-4467.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Passarelli A. L., Miller L. K. Identification of genes encoding late expression factors located between 56.0 and 65.4 map units of the Autographa californica nuclear polyhedrosis virus genome. Virology. 1993 Dec;197(2):704–714. doi: 10.1006/viro.1993.1646. [DOI] [PubMed] [Google Scholar]
  15. Passarelli A. L., Miller L. K. Three baculovirus genes involved in late and very late gene expression: ie-1, ie-n, and lef-2. J Virol. 1993 Apr;67(4):2149–2158. doi: 10.1128/jvi.67.4.2149-2158.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rice W. C., Miller L. K. Baculovirus transcription in the presence of inhibitors and in nonpermissive Drosophila cells. Virus Res. 1986 Nov;6(2):155–172. doi: 10.1016/0168-1702(86)90047-x. [DOI] [PubMed] [Google Scholar]
  17. Scafe C., Martin C., Nonet M., Podos S., Okamura S., Young R. A. Conditional mutations occur predominantly in highly conserved residues of RNA polymerase II subunits. Mol Cell Biol. 1990 Mar;10(3):1270–1275. doi: 10.1128/mcb.10.3.1270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schultz P., Célia H., Riva M., Sentenac A., Oudet P. Three-dimensional model of yeast RNA polymerase I determined by electron microscopy of two-dimensional crystals. EMBO J. 1993 Jul;12(7):2601–2607. doi: 10.1002/j.1460-2075.1993.tb05920.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thiem S. M., Miller L. K. Identification, sequence, and transcriptional mapping of the major capsid protein gene of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 1989 May;63(5):2008–2018. doi: 10.1128/jvi.63.5.2008-2018.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tomalski M. D., Wu J. G., Miller L. K. The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology. 1988 Dec;167(2):591–600. [PubMed] [Google Scholar]
  21. Wilson M. E., Mainprize T. H., Friesen P. D., Miller L. K. Location, transcription, and sequence of a baculovirus gene encoding a small arginine-rich polypeptide. J Virol. 1987 Mar;61(3):661–666. doi: 10.1128/jvi.61.3.661-666.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES