Abstract
Objective: Our goal was to determine the in vitro susceptibility of Ureaplasma urealyticum and Mycoplasma hominis isolates to several antibiotics in Argentina.
Methods: Ninety-four strains of U. urealyticum and 18 strains of M. hominis isolated from cervical and urethral specimens were studied. Broth microdilution and agar dilution tests for minocycline, tetracycline, erythromycin, ciprofloxacin, and ofloxacin were performed.
Results: Both methods proved to be reliable and reproducible for U. urealyticum and M. hominis, with no major differences in results. The U. urealyticurn strains were inhibited by erythromycin at MICs ranging from ≤0.5 to >8 μ/ml. Ofloxacin showed the highest activity against this latter organism. No differences between tetracycline and minocycline MICs were observed with U. urealyticum. Two M. hominis strains displaying high MICs both to tetracycline and to minocycline were detected.
Conclusions: The emerging resistance of mycoplasmas to certain antibiotics emphasizes the need to undertake further surveillance studies on the clinical isolates of such organisms.
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Selected References
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- Cassell G. H., Crouse D. T., Waites K. B., Rudd P. T., Davis J. K. Does Ureaplasma urealyticum cause respiratory disease in newborns? Pediatr Infect Dis J. 1988 Aug;7(8):535–541. [PubMed] [Google Scholar]
- Cassell G. H., Davis J. K., Waites K. B., Rudd P. T., Talkington D., Crouse D., Horowitz S. A. Pathogenesis and significance of urogenital mycoplasmal infections. Adv Exp Med Biol. 1987;224:93–115. doi: 10.1007/978-1-4684-8932-3_10. [DOI] [PubMed] [Google Scholar]
- Cassell G. H., Waites K. B., Crouse D. T., Rudd P. T., Canupp K. C., Stagno S., Cutter G. R. Association of Ureaplasma urealyticum infection of the lower respiratory tract with chronic lung disease and death in very-low-birth-weight infants. Lancet. 1988 Jul 30;2(8605):240–245. doi: 10.1016/s0140-6736(88)92536-6. [DOI] [PubMed] [Google Scholar]
- Cummings M. C., McCormack W. M. Increase in resistance of Mycoplasma hominis to tetracyclines. Antimicrob Agents Chemother. 1990 Dec;34(12):2297–2299. doi: 10.1128/aac.34.12.2297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evans R. T., Taylor-Robinson D. The incidence of tetracycline-resistant strains of Ureaplasma urealyticum. J Antimicrob Chemother. 1978 Jan;4(1):57–63. doi: 10.1093/jac/4.1.57. [DOI] [PubMed] [Google Scholar]
- Kenny G. E., Hooton T. M., Roberts M. C., Cartwright F. D., Hoyt J. Susceptibilities of genital mycoplasmas to the newer quinolones as determined by the agar dilution method. Antimicrob Agents Chemother. 1989 Jan;33(1):103–107. doi: 10.1128/aac.33.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robertson J. A., Coppola J. E., Heisler O. R. Standardized method for determining antimicrobial susceptibility of strains of Ureaplasma urealyticum and their response to tetracycline, erythromycin, and rosaramicin. Antimicrob Agents Chemother. 1981 Jul;20(1):53–58. doi: 10.1128/aac.20.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waites K. B., Cassell G. H., Canupp K. C., Fernandes P. B. In vitro susceptibilities of mycoplasmas and ureaplasmas to new macrolides and aryl-fluoroquinolones. Antimicrob Agents Chemother. 1988 Oct;32(10):1500–1502. doi: 10.1128/aac.32.10.1500. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waites K. B., Duffy L. B., Schmid T., Crabb D., Pate M. S., Cassell G. H. In vitro susceptibilities of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum to sparfloxacin and PD 127391. Antimicrob Agents Chemother. 1991 Jun;35(6):1181–1185. doi: 10.1128/aac.35.6.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]