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. 1994 Aug;68(8):5270–5274. doi: 10.1128/jvi.68.8.5270-5274.1994

Characterization of mpl cytoplasmic domain sequences required for myeloproliferative leukemia virus pathogenicity.

L Bénit 1, G Courtois 1, M Charon 1, P Varlet 1, I Dusanter-Fourt 1, S Gisselbrecht 1
PMCID: PMC236472  PMID: 8035524

Abstract

v-mpl is a truncated form of a receptor-like chain which belongs to the cytokine receptor superfamily. This sequence has been transduced in the myeloproliferative leukemia virus as an env-mpl fusion gene responsible for an acute myeloproliferative disorder in mice. We constructed a series of viral mutants in the mpl sequence. Analysis of their oncogenic potential in vivo indicated that a critical 69-amino-acid-long cytoplasmic domain of v-Mpl is required for myoproliferative leukemia virus pathogenicity. We also developed an in vitro assay and showed that expression of the env-mpl gene confers growth factor independence to murine as well as to human hematopoietic growth factor-dependent cell lines. These findings strongly suggest that v-Mpl delivers a constitutive proliferative signal through a limited region of its cytoplasmic domain.

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Selected References

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