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. 1992;1(1):49–54. doi: 10.1155/S0962935192000103

Neutrophil recruitment inhibitory factor: a possible candidate for a novel cytokine

W M S C Tamashiro 1, B M Tavares-Murta 1, F Q Cunha 1, M C Roque-Barreira 2, R M D Nogueira 1, S H Ferreira 1,✉
PMCID: PMC2365323  PMID: 18475441

Abstract

Inhibitory effect upon neutrophil migration to the inflammatory focus was previously detected in the cell-free incubation fluid of lipopolysaccharide (LPS)-stimulated macrophage monolayers. In the present study we showed that the neutrophil recruitment inhibitory activity from this supernatant was mainly detected in a fraction (P2) obtained by gel filtration chromatography on Sephacryl S-300. P2 fraction was able to inhibit ‘in vivo’ neutrophil emigration induced by different inflammatory stimuli, but it did not affect ‘in vitro’ neutrophil chemotaxis induced by FMLP. When injected intravenously, P2 inhibited oedema induced by carrageenin or immunological stimulus but not the oedema induced by dextran, thus affecting cell-dependent inflammatory responses. It was observed that P2 also induced neutrophil migration when injected locally in peritoneal cavities. This activity was significantly reduced by pretreatment of the animals with dexamethasone. Cytokines, such as IL-8 and TNF-α that are known to exhibit inhibitory effect upon neutrophil migration, were not detected in P2 fraction by highly sensitive assays. Overall the results suggest the existence of a novel cytokine exhibiting ‘in vivo’ neutrophil inhibitory activity, referred as NRIF.

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Selected References

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  1. Cannon J. G., Tompkins R. G., Gelfand J. A., Michie H. R., Stanford G. G., van der Meer J. W., Endres S., Lonnemann G., Corsetti J., Chernow B. Circulating interleukin-1 and tumor necrosis factor in septic shock and experimental endotoxin fever. J Infect Dis. 1990 Jan;161(1):79–84. doi: 10.1093/infdis/161.1.79. [DOI] [PubMed] [Google Scholar]
  2. Cicco N. A., Lindemann A., Content J., Vandenbussche P., Lübbert M., Gauss J., Mertelsmann R., Herrmann F. Inducible production of interleukin-6 by human polymorphonuclear neutrophils: role of granulocyte-macrophage colony-stimulating factor and tumor necrosis factor-alpha. Blood. 1990 May 15;75(10):2049–2052. [PubMed] [Google Scholar]
  3. Cunha F. Q., Ferreira S. H. The release of a neutrophil chemotactic factor from peritoneal macrophages by endotoxin: inhibition by glucocorticoids. Eur J Pharmacol. 1986 Sep 23;129(1-2):65–76. doi: 10.1016/0014-2999(86)90337-7. [DOI] [PubMed] [Google Scholar]
  4. Cybulsky M. I., Chan M. K., Movat H. Z. Acute inflammation and microthrombosis induced by endotoxin, interleukin-1, and tumor necrosis factor and their implication in gram-negative infection. Lab Invest. 1988 Apr;58(4):365–378. [PubMed] [Google Scholar]
  5. Cybulsky M. I., McComb D. J., Movat H. Z. Protein synthesis dependent and independent mechanisms of neutrophil emigration. Different mechanisms of inflammation in rabbits induced by interleukin-1, tumor necrosis factor alpha or endotoxin versus leukocyte chemoattractants. Am J Pathol. 1989 Jul;135(1):227–237. [PMC free article] [PubMed] [Google Scholar]
  6. Espevik T., Nissen-Meyer J. A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J Immunol Methods. 1986 Dec 4;95(1):99–105. doi: 10.1016/0022-1759(86)90322-4. [DOI] [PubMed] [Google Scholar]
  7. Faccioli L. H., Souza G. E., Cunha F. Q., Poole S., Ferreira S. H. Recombinant interleukin-1 and tumor necrosis factor induce neutrophil migration "in vivo" by indirect mechanisms. Agents Actions. 1990 Jun;30(3-4):344–349. doi: 10.1007/BF01966298. [DOI] [PubMed] [Google Scholar]
  8. Ferreira S. H. A new method for measuring variations of rat paw volume. J Pharm Pharmacol. 1979 Sep;31(9):648–648. doi: 10.1111/j.2042-7158.1979.tb13616.x. [DOI] [PubMed] [Google Scholar]
  9. Gimbrone M. A., Jr, Obin M. S., Brock A. F., Luis E. A., Hass P. E., Hébert C. A., Yip Y. K., Leung D. W., Lowe D. G., Kohr W. J. Endothelial interleukin-8: a novel inhibitor of leukocyte-endothelial interactions. Science. 1989 Dec 22;246(4937):1601–1603. doi: 10.1126/science.2688092. [DOI] [PubMed] [Google Scholar]
  10. Gregory H., Young J., Schröder J. M., Mrowietz U., Christophers E. Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP). Biochem Biophys Res Commun. 1988 Mar 15;151(2):883–890. doi: 10.1016/s0006-291x(88)80364-4. [DOI] [PubMed] [Google Scholar]
  11. Lo T. N., Almeida A. P., Beaven M. A. Dextran and carrageenan evoke different inflammatory responses in rat with respect to composition of infiltrates and effect of indomethacin. J Pharmacol Exp Ther. 1982 Apr;221(1):261–267. [PubMed] [Google Scholar]
  12. Macklem P. T. A hypothesis linking bronchial hyperreactivity and airway inflammation: implications for therapy. Ann Allergy. 1990 Feb;64(2 Pt 1):113–116. [PubMed] [Google Scholar]
  13. Nakamura H., Motoyoshi S., Kadokawa T. Anti-inflammatory action of interleukin 1 through the pituitary-adrenal axis in rats. Eur J Pharmacol. 1988 Jun 22;151(1):67–73. doi: 10.1016/0014-2999(88)90693-0. [DOI] [PubMed] [Google Scholar]
  14. Opp M., Obal F., Jr, Cady A. B., Johannsen L., Krueger J. M. Interleukin-6 is pyrogenic but not somnogenic. Physiol Behav. 1989 May;45(5):1069–1072. doi: 10.1016/0031-9384(89)90239-4. [DOI] [PubMed] [Google Scholar]
  15. Ribeiro R. A., Cunha F. Q., Ferreira S. H. Recombinant gamma interferon causes neutrophil migration mediated by the release of a macrophage neutrophil chemotactic factor. Int J Exp Pathol. 1990 Oct;71(5):717–725. [PMC free article] [PubMed] [Google Scholar]
  16. Ribeiro R. A., Flores C. A., Cunha F. Q., Ferreira S. H. IL-8 causes in vivo neutrophil migration by a cell-dependent mechanism. Immunology. 1991 Aug;73(4):472–477. [PMC free article] [PubMed] [Google Scholar]
  17. Strober W., James S. P. The interleukins. Pediatr Res. 1988 Nov;24(5):549–557. doi: 10.1203/00006450-198811000-00001. [DOI] [PubMed] [Google Scholar]
  18. Tavares B. M., Cunha F. Q., Ferreira S. H. Macrophages stimulated with lipopolysaccharide release a selective neutrophil recruitment inhibitory factor: an in vivo demonstration. Braz J Med Biol Res. 1989;22(6):733–736. [PubMed] [Google Scholar]
  19. Ulich T. R., Yin S., Guo K., Yi E. S., Remick D., del Castillo J. Intratracheal injection of endotoxin and cytokines. II. Interleukin-6 and transforming growth factor beta inhibit acute inflammation. Am J Pathol. 1991 May;138(5):1097–1101. [PMC free article] [PubMed] [Google Scholar]
  20. Walz A., Peveri P., Aschauer H., Baggiolini M. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Biophys Res Commun. 1987 Dec 16;149(2):755–761. doi: 10.1016/0006-291x(87)90432-3. [DOI] [PubMed] [Google Scholar]

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