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. 1992;1(5):329–333. doi: 10.1155/S0962935192000498

IL-1β and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture

Agnes Ribeiro 1, Marise Mangeney 1, Isabelle Bernard 2, Jean Chambaz 1, Francisco Delers 1,2,✉
PMCID: PMC2365358  PMID: 18475480

Abstract

Incubation of rat hepatocytes in primary culture with IL-1β at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (−35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1β (10 units/ml) or IL-6 (100 units/ml). These results suggest that inflammatory cytokines IL-1β and IL-6 modulate the expression of apoE gene in cultured rat hepatocytes, at a concentration that does not induce the acute phase response.

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Selected References

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