Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1993;2(1):53–57. doi: 10.1155/S0962935193000079

Production of oxygen free radicals by Ehrlich ascites tumour cells: effect of lipids

Gopal K Marathe 1,✉, Cletus J M D'Souza 1
PMCID: PMC2365376  PMID: 18475503

Abstract

Phorbol-12-myristate-13-acetate (PMA), calcium ionophore A23187 and platelet activating factor (PAF) stimulated the generation of oxygen free radicals (nitro-blue tetrazolium reduction) in Ehrlich ascites tumour (EAT) cells. PAF was effective at an optimal concentration of 4 μM, but was inhibited by BN 52021, a specific PAF antagonist. Lyso-PAF was ineffective. Inclusion of different lipids during incubation prior to the addition of PAF, resulted in the activation/inhibition of free radical generation. Among the phospholipids at a concentration of 50 μg/ml, the order of activation was phosphatidylserine > phosphatidylglycerol > phosphoinositides > phosphatidylinositol > phosphatidylethanolamine. Phosphatidylcholine was not effective, while sphingolipids were inhibitory. In addition, Ehrlich ascites tumour cells grown in mice under marginal vitamin A deficiency, showed an augmented production of free radicals compared to control cells. This was suppressed by exogenous addition of vitamin A or superoxide dismutase. These results suggest that membrane lipids and dietary factors like vitamin A probably function as physiological modulators in regulating the free radical generation.

Full Text

The Full Text of this article is available as a PDF (392.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baggiolini M., Dewald B. Stimulus amplification by PAF and LTB4 in human neutrophils. Pharmacol Res Commun. 1986 Aug;18 (Suppl):51–59. doi: 10.1016/0031-6989(86)90038-x. [DOI] [PubMed] [Google Scholar]
  2. Baggiolini M., Wymann M. P. Turning on the respiratory burst. Trends Biochem Sci. 1990 Feb;15(2):69–72. doi: 10.1016/0968-0004(90)90179-f. [DOI] [PubMed] [Google Scholar]
  3. Das U. N., Begin M. E., Ells G., Huang Y. S., Horrobin D. F. Polyunsaturated fatty acids augment free radical generation in tumor cells in vitro. Biochem Biophys Res Commun. 1987 May 29;145(1):15–24. doi: 10.1016/0006-291x(87)91281-2. [DOI] [PubMed] [Google Scholar]
  4. Gabig T. G., Babior B. M. The O2(-) -forming oxidase responsible for the respiratory burst in human neutrophils. Properties of the solubilized enzyme. J Biol Chem. 1979 Sep 25;254(18):9070–9074. [PubMed] [Google Scholar]
  5. Kartha V. N., Krishnamurthy S. Antioxidant function of vitamin A. Int J Vitam Nutr Res. 1977;47(4):394–401. [PubMed] [Google Scholar]
  6. Krause R. F., Beamer K. C., Lawrence C. Vitamin A deficiency and phospholipid metabolism. Am J Clin Nutr. 1969 Jan;22(1):27–32. doi: 10.1093/ajcn/22.1.27. [DOI] [PubMed] [Google Scholar]
  7. Krause R. F., Beamer K. C., Plow J. H. Phospholipid metabolism in vitamin A-deficientrats. J Nutr. 1971 Feb;101(2):161–168. doi: 10.1093/jn/101.2.161. [DOI] [PubMed] [Google Scholar]
  8. Logani M. K., Davies R. E. Lipid oxidation: biologic effects and antioxidants--a review. Lipids. 1980 Jun;15(6):485–495. doi: 10.1007/BF02534079. [DOI] [PubMed] [Google Scholar]
  9. Marathe G. K., Krishnakanta T. P., D'Souza C. J. PAF-acether (PAF) in Ehrlich ascites tumour cells. J Lipid Mediat. 1990 Sep-Oct;2(5):257–262. [PubMed] [Google Scholar]
  10. McCARTHY P. T., CERECEDO L. R. Vitamin A deficiency in the mouse. J Nutr. 1952 Mar;46(3):361–376. doi: 10.1093/jn/46.3.361. [DOI] [PubMed] [Google Scholar]
  11. Rouis M., Nigon F., Chapman M. J. Platelet activating factor is a potent stimulant of the production of active oxygen species by human monocyte-derived macrophages. Biochem Biophys Res Commun. 1988 Nov 15;156(3):1293–1301. doi: 10.1016/s0006-291x(88)80773-3. [DOI] [PubMed] [Google Scholar]
  12. Smith S. M., Levy N. S., Hayes C. E. Impaired immunity in vitamin A-deficient mice. J Nutr. 1987 May;117(5):857–865. doi: 10.1093/jn/117.5.857. [DOI] [PubMed] [Google Scholar]
  13. Stewart A. G., Harris T. Platelet-activating factor may participate in signal transduction processes in rabbit leukocytes. Lipids. 1991 Dec;26(12):1044–1049. doi: 10.1007/BF02536499. [DOI] [PubMed] [Google Scholar]
  14. Tamura M., Tamura T., Tyagi S. R., Lambeth J. D. The superoxide-generating respiratory burst oxidase of human neutrophil plasma membrane. Phosphatidylserine as an effector of the activated enzyme. J Biol Chem. 1988 Nov 25;263(33):17621–17626. [PubMed] [Google Scholar]
  15. Wilson E., Olcott M. C., Bell R. M., Merrill A. H., Jr, Lambeth J. D. Inhibition of the oxidative burst in human neutrophils by sphingoid long-chain bases. Role of protein kinase C in activation of the burst. J Biol Chem. 1986 Sep 25;261(27):12616–12623. [PubMed] [Google Scholar]
  16. Wymann M. P., von Tscharner V., Deranleau D. A., Baggiolini M. The onset of the respiratory burst in human neutrophils. Real-time studies of H2O2 formation reveal a rapid agonist-induced transduction process. J Biol Chem. 1987 Sep 5;262(25):12048–12053. [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES