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. 1993;2(3):235–241. doi: 10.1155/S0962935193000328

Immunomodulatory role of phagocyte-derived chloramines involving lymphocyte glutathione

Véronique Witko-Sarsat 1, Anh Thu Nguyen 1, Béatrice Descamps-Latscha 1,✉
PMCID: PMC2365400  PMID: 18475528

Abstract

This study shows that human lymphocytes markedly decrease chloramines (long-lived oxidants) generated by polymorphonuclear neutrophils (PMN) after stimulation by phorbol-myristate-acetate or opsonized zymosan. In a cell-free model, reduced glutathione (GSH) scavenged chloramines, giving rise to oxidized glutathione (GSSG). In the cell system, treatment of lymphocytes with autologous PMN-derived chloramines induced a profound decrease in their total and reduced glutathione (GSH) content and markedly inhibited their proliferate responses to concanavalin-A and, to a lesser extent, phytohaemagglutinin. It is concluded that (i) lymphocytes may play a defensive role against phagocyte-derived oxidative stress by scavenging chloramines, and (ii) as this effect which is mediated by GSH affects lymphocyte proliferative responses, it may help to elucidate the still obscure mechanisms of oxidative stress associated immunodeficiency.

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Selected References

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