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. 1993;2(3):205–210. doi: 10.1155/S0962935193000274

Desensitization of delayed-type hypersensitivity in mice: suppressive environment

Takashi Katsura 1, Kazuo Kobayashi 1,✉, Michio Hosaka 1, Sachiko Sugihara 1, Tsuyoshi Kasama 1, Keita Kasahara 1, Stanley Cohen 2, Takeshi Yoshida 3
PMCID: PMC2365409  PMID: 18475523

Abstract

The systemic injection of high doses of antigen into a preimmunized animal results in transient unresponsiveness of cell-mediated immune responses. This phenomenon is known as desensitization. Serum interleukin 2 (IL-2) activity was found transiently in desensitized mice at 3 h after the antigen challenge. These mice could not reveal antigen nonspecific delayed-type hypersensitivity (DTH) 1 d after the challenge. Specific suppression of DTH was observed at later stages. Sera from 3 h desensitized mice showed suppressive effects on DTH in preo immunized mice. Administration of recombinant IL-2 into preimmunized mice led to the failure of development of DTH to antigens. These observations suggest that IL-2 plays an important role in the suppressive environment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen S., Fisher B., Yoshida T., Bettigole R. E. Serum migration-inhibitory activity in patients with lymphoproliferative diseases. N Engl J Med. 1974 Apr 18;290(16):882–886. doi: 10.1056/NEJM197404182901605. [DOI] [PubMed] [Google Scholar]
  2. Cone R. E., Gerardi D. A., Davidoff J., Petty J., Kobayashi K., Cohen S. Quantitation of T cell antigen-binding molecules (TABM) in the sera of nonimmunized, immunized, and desensitized mice. J Immunol. 1987 Jan 1;138(1):234–239. [PubMed] [Google Scholar]
  3. Dwyer J. M., Kantor F. S. In vivo suppression of delayed hypersensitivity: prolongation of desensitization in guinea pigs. J Exp Med. 1975 Sep 1;142(3):588–599. doi: 10.1084/jem.142.3.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Janeway C. A., Jr, Carding S., Jones B., Murray J., Portoles P., Rasmussen R., Rojo J., Saizawa K., West J., Bottomly K. CD4+ T cells: specificity and function. Immunol Rev. 1988 Jan;101:39–80. doi: 10.1111/j.1600-065x.1988.tb00732.x. [DOI] [PubMed] [Google Scholar]
  5. Kobayashi K., Suko M., Yoshida T., Cohen S. Antigen-nonspecific and specific suppression of lymphokine production in desensitized guinea pigs. Cell Immunol. 1986 Feb;97(2):325–334. doi: 10.1016/0008-8749(86)90403-x. [DOI] [PubMed] [Google Scholar]
  6. Mosmann T. R., Coffman R. L. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol. 1989;7:145–173. doi: 10.1146/annurev.iy.07.040189.001045. [DOI] [PubMed] [Google Scholar]
  7. Nakauchi H., Ohno I., Kim M., Okumura K., Tada T. Establishment and functional analysis of a cloned, antigen-specific suppressor effector T cell line. J Immunol. 1984 Jan;132(1):88–94. [PubMed] [Google Scholar]
  8. Polák L., Turk J. L. Studies on the effect of systemic administration of sensitizers in guinea-pigs with contact sensitivity to inorganic metal compounds. I. The induction of immunological unresponsiveness in already sensitized animals. Clin Exp Immunol. 1968 Mar;3(3):245–251. [PMC free article] [PubMed] [Google Scholar]
  9. Schlossman S. F., Levin H. A., Rocklin R. E., David J. R. The compartmentalization of antigen-reactive lymphocytes in desensitized guinea pigs. J Exp Med. 1971 Sep 1;134(3 Pt 1):741–750. doi: 10.1084/jem.134.3.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sonozaki H., Papermaster V., Yoshida T., Cohen S. Desensitization: effects on cutaneous and peritoneal manifestations of delayed hypersensitivity in relation to lymphokine production. J Immunol. 1975 Dec;115(6):1657–1661. [PubMed] [Google Scholar]
  11. Suko M., Yoshida T., Cohen S. Desensitization. V: Suppression of MIF production by lymphokine-activated macrophages. Cell Immunol. 1985 Nov;96(1):49–60. doi: 10.1016/0008-8749(85)90339-9. [DOI] [PubMed] [Google Scholar]
  12. UHR J. W., PAPPENHEIMER A. M., Jr Delayed hypersensitivity. III. Specific desensitization of guinea pigs sensitized to protein antigens. J Exp Med. 1958 Dec 1;108(6):891–904. doi: 10.1084/jem.108.6.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilde D. B., Fitch F. W. Antigen-reactive cloned helper T cells. I. Unresponsiveness to antigenic restimulation develops after stimulation of cloned helper T cells. J Immunol. 1984 Apr;132(4):1632–1638. [PubMed] [Google Scholar]
  14. Yoshida T., Cohen S. Biological control of lymphokine function. Fed Proc. 1982 Jun;41(8):2480–2483. [PubMed] [Google Scholar]
  15. Yoshida T., Cohen S. Lymphokine activity in vivo in relation to circulating monocyte levels and delayed skin reactivity. J Immunol. 1974 Apr;112(4):1540–1547. [PubMed] [Google Scholar]
  16. Yoshida T., Siltzbach L. E., Masih N., Cohen S. Serum-migration inhibitory activity in patients with sarcoidosis. Clin Immunol Immunopathol. 1979 May;13(1):39–46. doi: 10.1016/0090-1229(79)90018-7. [DOI] [PubMed] [Google Scholar]

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