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. 1994;3(5):381–385. doi: 10.1155/S0962935194000530

Effect of Fluticasone propionate Aqueous Nasal Spray Treatment on Platelet Activating Factor and Eicosanoid Production By nasal Mucosa in Patients with A house Dust Mite Allergy

I M Garrelds 1,2,✉, T de Graaf-in 't Veld 2, A P H Jansen 2, R Gerth van Wijk 2, F J Zijlstra 1
PMCID: PMC2365576  PMID: 18475584

Abstract

The relationship between the release of platelet activating factor (PAF), leukotriene C4/D4/EE (LTC4/D4/E4) and prostaglandin D2 (PGD2) from nasal mucosa in vivo was examined in 24 rhinitis patients allergic to the house dust mite (HDM). During a double blind placebo controlled cross-over study 200 μg fluticasone propionate aqueous nasal spray (FPANS) was administered twice daily for two weeks. In response to allergen provocation (100, 1 000, 10 000 Bu/ml) and during the 9.5 h after this challenge the nasal fluid was obtained by washing the nose with saline and the levels of PAF, LTC4/D4/E4 and PGD2, as indicators of mediator release, were measured at the following time-points: baseline (t = − 1/2), allergen provocation with 10 000 Bu/ml (t = 0), 3.5 and 7.5 h (late phase). After allergen provocation the levels of the mediators increased in the nasal fluids of placebo treated patients (x-fold increase to baseline: PAF, 15; LTC4/D4/E4, 12; PGD2, 1.5). In fluids of patients treated with FPANS these levels tended to decrease. At the time of provocation the levels of PAF, LTC4/D4/E4 and PGD2 showed a significant correlation. The results indicate that these mediators can be used as markers of allergic reactions against house dust mites and that fluticasone propionate aqueous nasal spray tended to reduce the release of mediators of inflammation correlated with beneficial effects on clinical symptoms in this type of allergic reactions.

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Selected References

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  1. Adams G. K., 3rd, Lichtenstein L. M. Antagonism of antigen-induced contraction of guinea pig and human airways. Nature. 1977 Nov 17;270(5634):255–257. doi: 10.1038/270255a0. [DOI] [PubMed] [Google Scholar]
  2. Adams G. K., 3rd, Lichtenstein L. M. Indomethacin enhances response of human bronchus to antigen. Am Rev Respir Dis. 1985 Jan;131(1):8–10. doi: 10.1164/arrd.1985.131.1.8. [DOI] [PubMed] [Google Scholar]
  3. BROCKLEHURST W. E. The release of histamine and formation of a slow-reacting substance (SRS-A) during anaphylactic shock. J Physiol. 1960 Jun;151:416–435. doi: 10.1113/jphysiol.1960.sp006449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brink C., Grimaud C., Guillot C., Orehek J. The interaction between indomethacin and contractile agents on human isolated airway muscle. Br J Pharmacol. 1980 Jul;69(3):383–388. doi: 10.1111/j.1476-5381.1980.tb07026.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burka J. F., Ali M., McDonald J. W., Paterson N. A. Immunological and non-immunological synthesis and release of prostaglandins and thromboxanes from isolated guinea pig trachea. Prostaglandins. 1981 Nov;22(5):683–691. doi: 10.1016/0090-6980(81)90207-0. [DOI] [PubMed] [Google Scholar]
  6. Cho H. L., Ho P. P., Mihelich E. D., Snyder D. W. Relative potencies of 5-lipoxygenase inhibitors on antigen-induced contractions of guinea pig tracheal strips. J Pharmacol Methods. 1991 Dec;26(4):277–287. doi: 10.1016/0160-5402(91)90038-7. [DOI] [PubMed] [Google Scholar]
  7. Creese B. R., Temple D. M. The mediators of allergic contraction of human airway smooth muscle: a comparison of bronchial and lung parenchymal strip preparations. Clin Exp Pharmacol Physiol. 1986 Feb;13(2):103–111. doi: 10.1111/j.1440-1681.1986.tb00323.x. [DOI] [PubMed] [Google Scholar]
  8. Dahlén S. E., Björck T., Kumlin M., Granström E., Hedqvist P. Studies of leukotrienes as mediators in the human lung in vitro. Adv Prostaglandin Thromboxane Leukot Res. 1990;20:193–200. [PubMed] [Google Scholar]
  9. Dahlén S. E., Hansson G., Hedqvist P., Björck T., Granström E., Dahlén B. Allergen challenge of lung tissue from asthmatics elicits bronchial contraction that correlates with the release of leukotrienes C4, D4, and E4. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1712–1716. doi: 10.1073/pnas.80.6.1712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dunlop L. S., Smith A. P. Reduction of antigen-induced contraction of sensitized human bronchus in vitro by indomethacin. Br J Pharmacol. 1975 Aug;54(4):495–497. doi: 10.1111/j.1476-5381.1975.tb07596.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ellis J. L., Undem B. J. Role of cysteinyl-leukotrienes and histamine in mediating intrinsic tone in isolated human bronchi. Am J Respir Crit Care Med. 1994 Jan;149(1):118–122. doi: 10.1164/ajrccm.149.1.8111568. [DOI] [PubMed] [Google Scholar]
  12. Gorenne I., Labat C., Gascard J. P., Norel X., Müller-Peddinghaus R., Mohrs K. H., Taylor W. A., Gardiner P. J., Brink C. (R)-2-[4-(quinolin-2-yl-methoxy)phenyl]-2-cyclopentyl] acetic acid (BAY x1005), a potent leukotriene synthesis inhibitor: effects on anti-IgE challenge in human airways. J Pharmacol Exp Ther. 1994 Feb;268(2):868–872. [PubMed] [Google Scholar]
  13. Hand J. M., Schwalm S. F., Lewis A. J. Antagonism of antigen-induced contraction of isolated guinea-pig trachea by 5-lipoxygenase inhibitors. Int Arch Allergy Appl Immunol. 1986;79(1):8–13. doi: 10.1159/000233934. [DOI] [PubMed] [Google Scholar]
  14. Haye-Legrand I., Norel X., Labat C., Benveniste J., Brink C. Antigenic contraction of guinea pig tracheal preparations passively sensitized with monoclonal IgE: pharmacological modulation. Int Arch Allergy Appl Immunol. 1988;87(4):342–348. doi: 10.1159/000234699. [DOI] [PubMed] [Google Scholar]
  15. Jones T. R., Charette L., Denis D. Leukotriene and anti-IgE induced contractions of human isolated trachea: studies with leukotriene receptor antagonists and a novel 5-lipoxygenase inhibitor. Can J Physiol Pharmacol. 1988 Jun;66(6):762–768. doi: 10.1139/y88-121. [DOI] [PubMed] [Google Scholar]
  16. Muccitelli R. M., Tucker S. S., Hay D. W., Torphy T. J., Wasserman M. A. Is the guinea pig trachea a good in vitro model of human large and central airways? Comparison on leukotriene-, methacholine-, histamine- and antigen-induced contractions. J Pharmacol Exp Ther. 1987 Nov;243(2):467–473. [PubMed] [Google Scholar]
  17. Norel X., Labat C., Gardiner P. J., Brink C. Inhibitory effects of BAY u3405 on prostanoid-induced contractions in human isolated bronchial and pulmonary arterial muscle preparations. Br J Pharmacol. 1991 Nov;104(3):591–595. doi: 10.1111/j.1476-5381.1991.tb12474.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Piper P. J., Vane J. R. Release of additional factors in anaphylaxis and its antagonism by anti-inflammatory drugs. Nature. 1969 Jul 5;223(5201):29–35. doi: 10.1038/223029a0. [DOI] [PubMed] [Google Scholar]
  19. SCHILD H. O., HAWKINS D. F., MONGAR J. L., HERXHEIMER H. Reactions of isolated human asthmatic lung and bronchial tissue to a specific antigen; histamine release and muscular contraction. Lancet. 1951 Sep 1;2(6679):376–382. doi: 10.1016/s0140-6736(51)91554-1. [DOI] [PubMed] [Google Scholar]
  20. Saad M. H., Wilson M. A., Burka J. F. Release of leukotriene C4 from guinea pig trachea. Prostaglandins. 1983 May;25(5):741–752. doi: 10.1016/0090-6980(83)90128-4. [DOI] [PubMed] [Google Scholar]
  21. Undem B. J., Pickett W. C., Lichtenstein L. M., Adams G. K., 3rd The effect of indomethacin on immunologic release of histamine and sulfidopeptide leukotrienes from human bronchus and lung parenchyma. Am Rev Respir Dis. 1987 Nov;136(5):1183–1187. doi: 10.1164/ajrccm/136.5.1183. [DOI] [PubMed] [Google Scholar]
  22. Undem B. J., Pickett W. C., Lichtenstein L. M., Adams G. K., 3rd The effect of indomethacin on immunologic release of histamine and sulfidopeptide leukotrienes from human bronchus and lung parenchyma. Am Rev Respir Dis. 1987 Nov;136(5):1183–1187. doi: 10.1164/ajrccm/136.5.1183. [DOI] [PubMed] [Google Scholar]

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