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. 1994;3(6):433–437. doi: 10.1155/S096293519400061X

Endothelin-1 Production by Normal Human Cultured Keratinocytes and its Regulation

Hajime Inoue 1,✉, Nagaoki Wakisaka 1, Nobuhiro Tane 1, Kazumasa Ando 1, Emiko Isono 1, Miwa Yamanaka 1, Masaki Aihara 1, Hirotomo Ishida 1
PMCID: PMC2365585  PMID: 18475592

Abstract

The possibility that cultured keratinocytes produce endothelins were investigated. The results showed that cultured keratinocytes derived from normal human skin produce endothelin-1. Moreover, keratinocyte endothelin-1 production was completely inhibited by the presence of actinomycin D in the medium. As in the case of endothelial cells, recombinant interleukin-1β was capable of promoting endothelin-1 production in keratinocytes, whereas herapin inhibited it. Thrombin also inhibited endothelin-1 production. These results indicate that the mechanism of endothelin-1 production in keratinocytes is slightly different from the mechanism in vascular endothelial cells.

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Selected References

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  1. Boyce S. T., Ham R. G. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol. 1983 Jul;81(1 Suppl):33s–40s. doi: 10.1111/1523-1747.ep12540422. [DOI] [PubMed] [Google Scholar]
  2. Bull H. A., Bunker C. B., Terenghi G., Springall D. R., Zhao Y., Polak J. M., Dowd P. M. Endothelin-1 in human skin: immunolocalization, receptor binding, mRNA expression, and effects on cutaneous microvascular endothelial cells. J Invest Dermatol. 1991 Oct;97(4):618–623. doi: 10.1111/1523-1747.ep12483000. [DOI] [PubMed] [Google Scholar]
  3. Inoue H., Imokawa H., Yamanaka M., Isono E., Ando K., Kubota T., Aihara M., Ishida H. Detection of endothelin 1,2 and endothelin-like immunoreactant in wound surface and plasma in mice with thermal injury. Life Sci. 1993;52(25):PL291–PL296. doi: 10.1016/0024-3205(93)90695-y. [DOI] [PubMed] [Google Scholar]
  4. Katabami T., Shimizu M., Okano K., Yano Y., Nemoto K., Ogura M., Tsukamoto T., Suzuki S., Ohira K., Yamada Y. Intracellular signal transduction for interleukin-1 beta-induced endothelin production in human umbilical vein endothelial cells. Biochem Biophys Res Commun. 1992 Oct 30;188(2):565–570. doi: 10.1016/0006-291x(92)91093-6. [DOI] [PubMed] [Google Scholar]
  5. Kohno M., Horio T., Ikeda M., Yokokawa K., Fukui T., Yasunari K., Kurihara N., Takeda T. Angiotensin II stimulates endothelin-1 secretion in cultured rat mesangial cells. Kidney Int. 1992 Oct;42(4):860–866. doi: 10.1038/ki.1992.361. [DOI] [PubMed] [Google Scholar]
  6. Kupper T. S. Interleukin 1 and other human keratinocyte cytokines: molecular and functional characterization. Adv Dermatol. 1988;3:293–307. [PubMed] [Google Scholar]
  7. Kurihara H., Yoshizumi M., Sugiyama T., Takaku F., Yanagisawa M., Masaki T., Hamaoki M., Kato H., Yazaki Y. Transforming growth factor-beta stimulates the expression of endothelin mRNA by vascular endothelial cells. Biochem Biophys Res Commun. 1989 Mar 31;159(3):1435–1440. doi: 10.1016/0006-291x(89)92270-5. [DOI] [PubMed] [Google Scholar]
  8. Pentland A. P., Mahoney M. G. Keratinocyte prostaglandin synthesis is enhanced by IL-1. J Invest Dermatol. 1990 Jan;94(1):43–46. doi: 10.1111/1523-1747.ep12873337. [DOI] [PubMed] [Google Scholar]
  9. Schmitt A., Hauser C., Jaunin F., Dayer J. M., Saurat J. H. Normal epidermis contains high amounts of natural tissue IL 1 biochemical analysis by HPLC identifies a MW approximately 17 Kd form with a P1 5.7 and a MW approximately 30 Kd form. Lymphokine Res. 1986 Spring;5(2):105–118. [PubMed] [Google Scholar]
  10. Shichiri M., Hirata Y., Emori T., Ohta K., Nakajima T., Sato K., Sato A., Marumo F. Secretion of endothelin and related peptides from renal epithelial cell lines. FEBS Lett. 1989 Aug 14;253(1-2):203–206. doi: 10.1016/0014-5793(89)80959-7. [DOI] [PubMed] [Google Scholar]
  11. Takuwa N., Takuwa Y., Yanagisawa M., Yamashita K., Masaki T. A novel vasoactive peptide endothelin stimulates mitogenesis through inositol lipid turnover in Swiss 3T3 fibroblasts. J Biol Chem. 1989 May 15;264(14):7856–7861. [PubMed] [Google Scholar]
  12. Warner T. D., de Nucci G., Vane J. R. Rat endothelin is a vasodilator in the isolated perfused mesentery of the rat. Eur J Pharmacol. 1989 Jan 17;159(3):325–326. doi: 10.1016/0014-2999(89)90167-2. [DOI] [PubMed] [Google Scholar]
  13. Yada Y., Higuchi K., Imokawa G. Effects of endothelins on signal transduction and proliferation in human melanocytes. J Biol Chem. 1991 Sep 25;266(27):18352–18357. [PubMed] [Google Scholar]
  14. Yanagisawa M., Kurihara H., Kimura S., Tomobe Y., Kobayashi M., Mitsui Y., Yazaki Y., Goto K., Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988 Mar 31;332(6163):411–415. doi: 10.1038/332411a0. [DOI] [PubMed] [Google Scholar]
  15. Yohn J. J., Morelli J. G., Walchak S. J., Rundell K. B., Norris D. A., Zamora M. R. Cultured human keratinocytes synthesize and secrete endothelin-1. J Invest Dermatol. 1993 Jan;100(1):23–26. doi: 10.1111/1523-1747.ep12349932. [DOI] [PubMed] [Google Scholar]
  16. Yokokawa K., Mandal A. K., Kohno M., Horio T., Murakawa K., Yasunari K., Takeda T. Heparin suppresses endothelin-1 action and production in spontaneously hypertensive rats. Am J Physiol. 1992 Nov;263(5 Pt 2):R1035–R1041. doi: 10.1152/ajpregu.1992.263.5.R1035. [DOI] [PubMed] [Google Scholar]
  17. Yoshizawa T., Shinmi O., Giaid A., Yanagisawa M., Gibson S. J., Kimura S., Uchiyama Y., Polak J. M., Masaki T., Kanazawa I. Endothelin: a novel peptide in the posterior pituitary system. Science. 1990 Jan 26;247(4941):462–464. doi: 10.1126/science.2405487. [DOI] [PubMed] [Google Scholar]
  18. Yoshizumi M., Kurihara H., Morita T., Yamashita T., Oh-hashi Y., Sugiyama T., Takaku F., Yanagisawa M., Masaki T., Yazaki Y. Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells. Biochem Biophys Res Commun. 1990 Jan 15;166(1):324–329. doi: 10.1016/0006-291x(90)91948-r. [DOI] [PubMed] [Google Scholar]

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