Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1995 Jan;4(1):49–54. doi: 10.1155/S0962935195000093

Defective antigen presentation by monocytes in ESRD patients not responding to hepatitis B vaccination: impaired HBsAg internalization and expression of ICAM-1 and HLA-DR/Ia molecules

J Stachowski 1,2,✉, C Barth 1, M Pollok 1, J Michałkiewicz 3, K Madaliński 3, J Maciejewski 2, C A Baldamis 1
PMCID: PMC2365607  PMID: 18475616

Abstract

This study was undertaken to evaluate the monocyte function of uraemic non-responders to hepatitis B vaccination. Therefore, some parameters concerning antigen processing by monocytes (Mo) as antigen presenting cells (APC) were analysed. It was found that in uraemic non-responders, (1) the internalization of HBsAg by monocytes was significantly decreasjed—HBsAg complexed with specific IgG or as immune complex isolated from patients is better internalized compared with free HBsAg; (2) during antigen presentation the expression of adhesion (ICAM-1) and accessory (HLA-DR/Ia) molecules was significantly decreased in uraemic patients, especially in non-responders; and (3) impaired internalization of HBsAg as well as a decrease in ICAM-1 and HLA-DR/Ia expression, correlated well with the blunted proliferation of CD4+ T cells stimulated by autologous monocytes induced by HBsAg.

Full Text

The Full Text of this article is available as a PDF (643.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexiewicz J. M., Smogorzewski M., Fadda G. Z., Massry S. G. Impaired phagocytosis in dialysis patients: studies on mechanisms. Am J Nephrol. 1991;11(2):102–111. doi: 10.1159/000168284. [DOI] [PubMed] [Google Scholar]
  2. Alper C. A., Kruskall M. S., Marcus-Bagley D., Craven D. E., Katz A. J., Brink S. J., Dienstag J. L., Awdeh Z., Yunis E. J. Genetic prediction of nonresponse to hepatitis B vaccine. N Engl J Med. 1989 Sep 14;321(11):708–712. doi: 10.1056/NEJM198909143211103. [DOI] [PubMed] [Google Scholar]
  3. Altman A., Coggeshall K. M., Mustelin T. Molecular events mediating T cell activation. Adv Immunol. 1990;48:227–360. doi: 10.1016/s0065-2776(08)60756-7. [DOI] [PubMed] [Google Scholar]
  4. Assoian R. K., Fleurdelys B. E., Stevenson H. C., Miller P. J., Madtes D. K., Raines E. W., Ross R., Sporn M. B. Expression and secretion of type beta transforming growth factor by activated human macrophages. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6020–6024. doi: 10.1073/pnas.84.17.6020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beaurain G., Naret C., Marcon L., Grateau G., Drueke T., Urena P., Nelson D. L., Bach J. F., Chatenoud L. In vivo T cell preactivation in chronic uremic hemodialyzed and non-hemodialyzed patients. Kidney Int. 1989 Oct;36(4):636–644. doi: 10.1038/ki.1989.240. [DOI] [PubMed] [Google Scholar]
  6. Becker J. C., Dummer R., Hartmann A. A., Burg G., Schmidt R. E. Shedding of ICAM-1 from human melanoma cell lines induced by IFN-gamma and tumor necrosis factor-alpha. Functional consequences on cell-mediated cytotoxicity. J Immunol. 1991 Dec 15;147(12):4398–4401. [PubMed] [Google Scholar]
  7. Bouffard P., Lamelin J. P., Zoulim F., Pichoud C., Trepo C. Different forms of hepatitis B virus DNA and expression of HBV antigens in peripheral blood mononuclear cells in chronic hepatitis B. J Med Virol. 1990 Aug;31(4):312–317. doi: 10.1002/jmv.1890310413. [DOI] [PubMed] [Google Scholar]
  8. Brown S. E., Howard C. R., Zuckerman A. J., Steward M. W. Determination of the affinity of antibodies to hepatitis B surface antigen in human sera. J Immunol Methods. 1984 Aug 3;72(1):41–48. doi: 10.1016/0022-1759(84)90431-9. [DOI] [PubMed] [Google Scholar]
  9. Ceuppens J. L., Desmyter J. Effect of prostaglandin synthesis inhibition in vivo on the immune response following hepatitis B vaccination in normal subjects. Int J Immunopharmacol. 1987;9(7):803–810. doi: 10.1016/0192-0561(87)90076-2. [DOI] [PubMed] [Google Scholar]
  10. Dumann H., Meuer S. C., Köhler H. Uremic serum inhibits monocyte-dependent, but not interleukin-2-dependent steps of T cell proliferation. Nephron. 1990;56(2):162–165. doi: 10.1159/000186126. [DOI] [PubMed] [Google Scholar]
  11. Dumann H., Meuer S. C., Renschin G., Köhler H. Influence of thymopentin on antibody response, and monocyte and T cell function in hemodialysis patients who fail to respond to hepatitis B vaccination. Nephron. 1990;55(2):136–140. doi: 10.1159/000185941. [DOI] [PubMed] [Google Scholar]
  12. Dumann H., Meuer S., Meyer zum Büschenfelde K. H., Köhler H. Hepatitis B vaccination and interleukin 2 receptor expression in chronic renal failure. Kidney Int. 1990 Dec;38(6):1164–1168. doi: 10.1038/ki.1990.328. [DOI] [PubMed] [Google Scholar]
  13. Franco A., Barnaba V., Ruberti G., Benvenuto R., Balsano C., Musca A. Liver-derived T cell clones in autoimmune chronic active hepatitis: accessory cell function of hepatocytes expressing class II major histocompatibility complex molecules. Clin Immunol Immunopathol. 1990 Mar;54(3):382–394. doi: 10.1016/0090-1229(90)90052-r. [DOI] [PubMed] [Google Scholar]
  14. Geppert T. D., Davis L. S., Gur H., Wacholtz M. C., Lipsky P. E. Accessory cell signals involved in T-cell activation. Immunol Rev. 1990 Oct;117:5–66. doi: 10.1111/j.1600-065x.1990.tb00566.x. [DOI] [PubMed] [Google Scholar]
  15. Gibbons R. A., Martinez O. M., Garovoy M. R. Altered monocyte function in uremia. Clin Immunol Immunopathol. 1990 Jul;56(1):66–80. doi: 10.1016/0090-1229(90)90170-u. [DOI] [PubMed] [Google Scholar]
  16. Gregorek H., Jung H., Ułanowicz G., Madaliński K. Immune complexes in sera of children with HBV-mediated glomerulonephritis. Arch Immunol Ther Exp (Warsz) 1986;34(1):73–83. [PubMed] [Google Scholar]
  17. Johnson R. J., Couser W. G. Hepatitis B infection and renal disease: clinical, immunopathogenetic and therapeutic considerations. Kidney Int. 1990 Feb;37(2):663–676. doi: 10.1038/ki.1990.32. [DOI] [PubMed] [Google Scholar]
  18. Krämer A., Herth D., von Keyserlingk H. J., Ludwig W. D., Hampl H., Sommer D., Hahn E. G., Riecken E. O. Non-responsiveness to hepatitis-B vaccination: revaccination and immunogenetic typing. Klin Wochenschr. 1988 Aug 1;66(15):670–674. doi: 10.1007/BF01726924. [DOI] [PubMed] [Google Scholar]
  19. Köhler H., Dumann H., Meyer zum Büschenfelde K. H., Meuer S. Sekundärer Immundefekt bei Niereninsuffizienz am Beispiel der Hepatitis B-Impfung. Klin Wochenschr. 1988 Sep 15;66(18):865–872. doi: 10.1007/BF01728948. [DOI] [PubMed] [Google Scholar]
  20. Ladefoged J., Langhoff E. Accessory cell functions in mononuclear cell cultures uremic patients. Kidney Int. 1990 Jan;37(1):126–130. doi: 10.1038/ki.1990.17. [DOI] [PubMed] [Google Scholar]
  21. Langhoff E., Hofmann B., Odum N., Ladefoged J., Platz P., Ryder L. P., Svejgaard A. Kinetic analysis of interleukin 2 (IL-2) production and expression of IL-2 receptors by uraemic and normal lymphocytes. Scand J Immunol. 1987 Jan;25(1):29–36. doi: 10.1111/j.1365-3083.1987.tb01043.x. [DOI] [PubMed] [Google Scholar]
  22. Lerner R. A., Green N., Alexander H., Liu F. T., Sutcliffe J. G., Shinnick T. M. Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3403–3407. doi: 10.1073/pnas.78.6.3403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Meuer S. C., Dumann H., Meyer zum Büschenfelde K. H., Köhler H. Low-dose interleukin-2 induces systemic immune responses against HBsAg in immunodeficient non-responders to hepatitis B vaccination. Lancet. 1989 Jan 7;1(8628):15–18. doi: 10.1016/s0140-6736(89)91674-7. [DOI] [PubMed] [Google Scholar]
  24. Meuer S. C., Hauer M., Kurz P., Meyer zum Büschenfelde K. H., Köhler H. Selective blockade of the antigen-receptor-mediated pathway of T cell activation in patients with impaired primary immune responses. J Clin Invest. 1987 Sep;80(3):743–749. doi: 10.1172/JCI113129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pasek M., Goto T., Gilbert W., Zink B., Schaller H., MacKay P., Leadbetter G., Murray K. Hepatitis B virus genes and their expression in E. coli. Nature. 1979 Dec 6;282(5739):575–579. doi: 10.1038/282575a0. [DOI] [PubMed] [Google Scholar]
  26. Pol S., Legendre C., Mattlinger B., Berthelot P., Kreis H. Genetic basis of nonresponse to hepatitis B vaccine in hemodialyzed patients. J Hepatol. 1990 Nov;11(3):385–387. doi: 10.1016/0168-8278(90)90226-h. [DOI] [PubMed] [Google Scholar]
  27. Quiroga J. A., Castillo I., Porres J. C., Casado S., Sáez F., Gracia Martínez M., Gómez M., Inglada L., Sánchez-Sicilia L., Mora A. Recombinant gamma-interferon as adjuvant to hepatitis B vaccine in hemodialysis patients. Hepatology. 1990 Oct;12(4 Pt 1):661–663. doi: 10.1002/hep.1840120407. [DOI] [PubMed] [Google Scholar]
  28. Raskova J., Ghobrial I., Shea S. M., Ebert E. C., Eisinger R. P., Raska K., Jr T cells in patients undergoing chronic hemodialysis: mitogenic response, suppressor activity, and interleukin-2 production and receptor generation. Diagn Immunol. 1986;4(4):209–216. [PubMed] [Google Scholar]
  29. Ruiz P., Gomez F., Schreiber A. D. Impaired function of macrophage Fc gamma receptors in end-stage renal disease. N Engl J Med. 1990 Mar 15;322(11):717–722. doi: 10.1056/NEJM199003153221102. [DOI] [PubMed] [Google Scholar]
  30. Seth R., Raymond F. D., Makgoba M. W. Circulating ICAM-1 isoforms: diagnostic prospects for inflammatory and immune disorders. Lancet. 1991 Jul 13;338(8759):83–84. doi: 10.1016/0140-6736(91)90077-3. [DOI] [PubMed] [Google Scholar]
  31. Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
  32. Stachowski J., Michalkiewicz J., Gregorek H., Madalinski K., Maciejewski J. Influence of immune complexes containing HBsAg and HBeAg on IL-2 dependent human lymphocyte proliferation. Allerg Immunol (Leipz) 1990;36(1):47–60. [PubMed] [Google Scholar]
  33. Stachowski J., Pollok M., Barth C., Maciejewski J., Baldamus C. A. Non-responsiveness to hepatitis B vaccination in haemodialysis patients: association with impaired TCR/CD3 antigen receptor expression regulating co-stimulatory processes in antigen presentation and recognition. Nephrol Dial Transplant. 1994;9(2):144–152. [PubMed] [Google Scholar]
  34. Stachowski J., Pollok M., Burrichter H., Baldamus C. A. Immunodeficiency in ESRD-patients is linked to altered IL-2 receptor density on T cell subsets. J Clin Lab Immunol. 1991 Apr;34(4):171–177. [PubMed] [Google Scholar]
  35. Stachowski J., Pollok M., Burrichter H., Spithaler C., Baldamus C. A. Does uremic environment down-regulate T cell activation via TCR/CD3 antigen receptor complex? J Clin Lab Immunol. 1991 Sep;36(1):15–21. [PubMed] [Google Scholar]
  36. Stachowski J., Pollok M., Burrichter H., Spithaler C., Baldamus C. A. Signalling via the TCR/CD3 antigen receptor complex in uremia is limited by the receptors number. Nephron. 1993;64(3):369–375. doi: 10.1159/000187356. [DOI] [PubMed] [Google Scholar]
  37. Tsakolos N. D., Theoharides T. C., Hendler E. D., Goffinet J., Dwyer J. M., Whisler R. L., Askenase P. W. Immune defects in chronic renal impairment: evidence for defective regulation of lymphocyte response by macrophages from patients with chronic renal impairment on haemodialysis. Clin Exp Immunol. 1986 Jan;63(1):218–227. [PMC free article] [PubMed] [Google Scholar]
  38. Zeitz M. Hepatitis-B-Impfung bei Dialyse-patienten: Reversibilität der defekten Immunantwort durch gleichzeitige Gabe von Interleukin-2. Z Gastroenterol. 1990 Mar;28(3):179–180. [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES