Abstract
We investigated whether TNB alters colonic tissue levels of PAF at the expense of changes in colonic motility in the isolated perfused rabbit left colon. Colonic inflammation was induced by the intracolonic administration of 0.25 ml of 50% ethanol containing 30 mg of trinitrobenzene. Strain gauge transducers were sewn onto the serosal surface of the colon to evaluate colonic motility. TNB administration increased tissue levels of PAF and increased the average force of each colonic contraction. Pretreatment with the PAF receptor antagonist WEB-2170 prior to TNB infusion decreased tissue concentrations of PAF and ameliorated the altered motility parameters seen with TNB alone. These results suggest that PAF is stimulated by TNB and may participate in colonic dysmotility during inflammatory states.
Full Text
The Full Text of this article is available as a PDF (162.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kaminski D. L., Andrus C. H., German D., Deshpande Y. G. The role of prostanoids in the production of acute acalculous cholecystitis by platelet-activating factor. Ann Surg. 1990 Oct;212(4):455–461. doi: 10.1097/00000658-199010000-00008. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lauritsen K., Laursen L. S., Bukhave K., Rask-Madsen J. In vivo profiles of eicosanoids in ulcerative colitis, Crohn's colitis, and Clostridium difficile colitis. Gastroenterology. 1988 Jul;95(1):11–17. doi: 10.1016/0016-5085(88)90284-3. [DOI] [PubMed] [Google Scholar]
- Morteau O., More J., Pons L., Bueno L. Platelet-activating factor and interleukin 1 are involved in colonic dysmotility in experimental colitis in rats. Gastroenterology. 1993 Jan;104(1):47–56. doi: 10.1016/0016-5085(93)90834-y. [DOI] [PubMed] [Google Scholar]
- Pons L., Droy-Lefaix M. T., Braquet P., Buéno L. Involvement of platelet-activating factor (PAF) in endotoxin-induced intestinal motor disturbances in rats. Life Sci. 1989;45(6):533–541. doi: 10.1016/0024-3205(89)90104-5. [DOI] [PubMed] [Google Scholar]
- Pons L., Droy-Lefaix M. T., Bueno L. Leukotriene D4 participates in colonic transit disturbances induced by intracolonic administration of trinitrobenzene sulfonic acid in rats. Gastroenterology. 1992 Jan;102(1):149–156. doi: 10.1016/0016-5085(92)91794-5. [DOI] [PubMed] [Google Scholar]