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. 1995 Mar;4(2):95–97. doi: 10.1155/S0962935195000159

Group II phospholipase A2 in human gingiva with periodontal disease

H Shinohara 1, T Komatsubara 2, H Tojo 2, M Okamoto 2, S Nishikawa 1, T Nagata 1, H Ishida 1,✉
PMCID: PMC2365617  PMID: 18475622

Abstract

Fourteen kilodalton phospholipase A2 molecules (PLA2) are classified into two groups, I- and II-PLA2, and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA2 activity in gingival crevicular fluid (GCF) of patients with periodontal disease, without determining the group of the enzyme involved. In this study, the activity, groups and levels of enzyme in gingiva taken from 13 sites of periodontal disease were determined using both biochemical and radioimmunological methods. A linear correlation between the activity and the level of II-PLA2 was observed. No I-PLA2 was found in any of the samples tested. These data suggest that the PLA2 activity found in the GCF of patients with periodontal disease does not belong to the I-PLA2 but to the II-PLA2 group.

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Selected References

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  1. Bomalaski J. S., Fallon M., Turner R. A., Crooke S. T., Meunier P. C., Clark M. A. Identification and isolation of a phospholipase A2 activating protein in human rheumatoid arthritis synovial fluid: induction of eicosanoid synthesis and an inflammatory response in joints injected in vivo. J Lab Clin Med. 1990 Dec;116(6):814–825. [PubMed] [Google Scholar]
  2. Heinrikson R. L., Krueger E. T., Keim P. S. Amino acid sequence of phospholipase A2-alpha from the venom of Crotalus adamanteus. A new classification of phospholipases A2 based upon structural determinants. J Biol Chem. 1977 Jul 25;252(14):4913–4921. [PubMed] [Google Scholar]
  3. Ishida H., Shinohara H., Amabe Y., Tojo H., Nagata T., Wakano Y. Effects of interleukin 1 beta, tumor necrosis factor alpha and transforming growth factor beta on group II phospholipase A2 activity in rat gingival fibroblasts. J Periodontal Res. 1993 Nov;28(6 Pt 2):517–520. doi: 10.1111/j.1600-0765.1993.tb02116.x. [DOI] [PubMed] [Google Scholar]
  4. Ishida H., Shinohara H., Nagata T., Nishikawa S., Wakano Y. Phospholipase A(2) Activity in Gingival Crevicular Fluid from Patients with Periodontal Disease: A possible Marker of Disease Activity. Mediators Inflamm. 1994;3(1):17–21. doi: 10.1155/S0962935194000037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Matsuda Y., Ogawa M., Sakamoto K., Yamashita S., Kanda A., Kohno M., Yoshida N., Nishijima J., Murata A., Mori T. Development of a radioimmunoassay for human group-II phospholipase A2 and demonstration of postoperative elevation. Enzyme. 1991;45(4):200–208. doi: 10.1159/000468890. [DOI] [PubMed] [Google Scholar]
  6. Minami T., Tojo H., Shinomura Y., Komatsubara T., Matsuzawa Y., Okamoto M. Elevation of phospholipase A2 protein in sera of patients with Crohn's disease and ulcerative colitis. Am J Gastroenterol. 1993 Jul;88(7):1076–1080. [PubMed] [Google Scholar]
  7. Minami T., Tojo H., Shinomura Y., Tarui S., Okamoto M. Raised serum activity of phospholipase A2 immunochemically related to group II enzyme in inflammatory bowel disease: its correlation with disease activity of Crohn's disease and ulcerative colitis. Gut. 1992 Jul;33(7):914–921. doi: 10.1136/gut.33.7.914. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murakami M., Kudo I., Umeda M., Matsuzawa A., Takeda M., Komada M., Fujimori Y., Takahashi K., Inoue K. Detection of three distinct phospholipases A2 in cultured mast cells. J Biochem. 1992 Feb;111(2):175–181. doi: 10.1093/oxfordjournals.jbchem.a123733. [DOI] [PubMed] [Google Scholar]
  9. Pruzanski W., Keystone E. C., Sternby B., Bombardier C., Snow K. M., Vadas P. Serum phospholipase A2 correlates with disease activity in rheumatoid arthritis. J Rheumatol. 1988 Sep;15(9):1351–1355. [PubMed] [Google Scholar]
  10. Shinohara H., Amabe Y., Komatsubara T., Tojo H., Okamoto M., Wakano Y., Ishida H. Group II phospholipase A2 induced by interleukin-1 beta in cultured rat gingival fibroblasts. FEBS Lett. 1992 Jun 8;304(1):69–72. doi: 10.1016/0014-5793(92)80591-4. [DOI] [PubMed] [Google Scholar]
  11. Stashenko P., Fujiyoshi P., Obernesser M. S., Prostak L., Haffajee A. D., Socransky S. S. Levels of interleukin 1 beta in tissue from sites of active periodontal disease. J Clin Periodontol. 1991 Aug;18(7):548–554. doi: 10.1111/j.1600-051x.1991.tb00088.x. [DOI] [PubMed] [Google Scholar]
  12. Suwa Y., Kudo I., Imaizumi A., Okada M., Kamimura T., Suzuki Y., Chang H. W., Hara S., Inoue K. Proteinaceous inhibitors of phospholipase A2 purified from inflammatory sites in rats. Proc Natl Acad Sci U S A. 1990 Apr;87(7):2395–2399. doi: 10.1073/pnas.87.7.2395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vadas P., Keystone J., Stefanski E., Scott K., Pruzanski W. Induction of circulating group II phospholipase A2 expression in adults with malaria. Infect Immun. 1992 Sep;60(9):3928–3931. doi: 10.1128/iai.60.9.3928-3931.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vadas P., Scott K., Smith G., Rajkovic I., Stefanski E., Schouten B. D., Singh R., Pruzanski W. Serum phospholipase A2 enzyme activity and immunoreactivity in a prospective analysis of patients with septic shock. Life Sci. 1992;50(11):807–811. doi: 10.1016/0024-3205(92)90186-s. [DOI] [PubMed] [Google Scholar]
  15. van den Bosch H. Intracellular phospholipases A. Biochim Biophys Acta. 1980 Sep 30;604(2):191–246. doi: 10.1016/0005-2736(80)90574-x. [DOI] [PubMed] [Google Scholar]

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