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. 1995 Mar;4(2):90–94. doi: 10.1155/S0962935195000147

The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells

M K Nag 1, Y G Deshpande 1, D Beck 1, A Li 1, D L Kaminski 1,✉
PMCID: PMC2365627  PMID: 18475621

Abstract

It has been demonstrated that lysolecithin (lysophosphatidyl choline, LPC) produces experimental cholecystitis in cats mediated by arachidonic acid metabolites. LPC is a cytolytic agent that has been postulated as a contributing factor in the development of cholecystitis in humans. The purpose of this research was to evaluate the effect of LPC on human gall-bladder mucosal cell phospholipase A2 and cyclooxygenase activity. Gall-bladder mucosal cells were isolated from the gall-bladders of patients undergoing routine cholecystectomy. Fresh, isolated cells were maintained in tissue culture and stimulated with varying doses of LPC. Platelet-activating factor concentration was quantitated as an index of phospholipase A2 activity and prostanoids were measured as an index of cyclooxygenase activity. Also, the effect of LPC on cyclooxygenase 1 and 2 expression in microsomal protein was evaluated. LPC caused dose related increases in 6-keto-PGF1α and PAF produced by human gall-bladder mucosal cells. Exposure of human gall-bladder mucosal cells to LPC failed to elicit expression of constitutive cyclooxygenase-1, while the expression of inducible cyclooxygenase-2 was increased. The results of this study indicate that LPC induces the formation of prostanoids and PAF by human gall-bladder mucosal cells, suggesting that this substance may promote the development of gall-bladder inflammation.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Carey M. C., Cahalane M. J. Whither biliary sludge? Gastroenterology. 1988 Aug;95(2):508–523. doi: 10.1016/0016-5085(88)90513-6. [DOI] [PubMed] [Google Scholar]
  3. Estrada C., Gómez C., Martín C., Moncada S., González C. Nitric oxide mediates tumor necrosis factor-alpha cytotoxicity in endothelial cells. Biochem Biophys Res Commun. 1992 Jul 15;186(1):475–482. doi: 10.1016/s0006-291x(05)80832-0. [DOI] [PubMed] [Google Scholar]
  4. German D., Barcia J., Brems J., Merenda G., Kaminski D. L. Effect of bradykinin on feline gallbladder water transport and prostanoid formation. Dig Dis Sci. 1989 Nov;34(11):1770–1776. doi: 10.1007/BF01540057. [DOI] [PubMed] [Google Scholar]
  5. Gottfries A. Lysolecithin: a factor in the pathogenesis of acute cholecystitis? An experimental study on rabbits based on clinical findings in humans. Acta Chir Scand. 1969;135(3):213–217. [PubMed] [Google Scholar]
  6. Gottfries A., Nilsson S., Samuelsson B., Scherstén T. Phospholipids in human hepatic bile, gall bladder bile, and plasma in cases with acute cholecystitis. Scand J Clin Lab Invest. 1968;21(2):168–176. doi: 10.3109/00365516809084279. [DOI] [PubMed] [Google Scholar]
  7. Ialenti A., Ianaro A., Moncada S., Di Rosa M. Modulation of acute inflammation by endogenous nitric oxide. Eur J Pharmacol. 1992 Feb 11;211(2):177–182. doi: 10.1016/0014-2999(92)90526-a. [DOI] [PubMed] [Google Scholar]
  8. Kaminski D. L., Amir G., Deshpande Y. G., Beck D., Li A. P. Studies on the etiology of acute acalculous cholecystitis: the effect of lipopolysaccharide on human gallbladder mucosal cells. Prostaglandins. 1994 Apr;47(4):319–330. doi: 10.1016/0090-6980(94)90026-4. [DOI] [PubMed] [Google Scholar]
  9. Kaminski D. L., Andrus C. H., German D., Deshpande Y. G. The role of prostanoids in the production of acute acalculous cholecystitis by platelet-activating factor. Ann Surg. 1990 Oct;212(4):455–461. doi: 10.1097/00000658-199010000-00008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaminski D. L., Daneshmand H., Dean P., Deshpande Y. G. Prostanoids and leukotrienes in experimental feline cholecystitis. Hepatology. 1990 Jun;11(6):1003–1009. doi: 10.1002/hep.1840110615. [DOI] [PubMed] [Google Scholar]
  11. Kaminski D. L., Feinstein W. K., Deshpande Y. G. The production of experimental cholecystitis by endotoxin. Prostaglandins. 1994 Mar;47(3):233–245. doi: 10.1016/0090-6980(94)90063-9. [DOI] [PubMed] [Google Scholar]
  12. LaMorte W. W., LaMont J. T., Hale W., Booker M. L., Scott T. E., Turner B. Gallbladder prostaglandins and lysophospholipids as mediators of mucin secretion during cholelithiasis. Am J Physiol. 1986 Nov;251(5 Pt 1):G701–G709. doi: 10.1152/ajpgi.1986.251.5.G701. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Maier J. A., Hla T., Maciag T. Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells. J Biol Chem. 1990 Jul 5;265(19):10805–10808. [PubMed] [Google Scholar]
  15. Matsudaira P. T., Burgess D. R. SDS microslab linear gradient polyacrylamide gel electrophoresis. Anal Biochem. 1978 Jul 1;87(2):386–396. doi: 10.1016/0003-2697(78)90688-7. [DOI] [PubMed] [Google Scholar]
  16. Mayer R. J., Marshall L. A. New insights on mammalian phospholipase A2(s); comparison of arachidonoyl-selective and -nonselective enzymes. FASEB J. 1993 Feb 1;7(2):339–348. doi: 10.1096/fasebj.7.2.8440410. [DOI] [PubMed] [Google Scholar]
  17. Neiderhiser D., Thornell E., Björck S., Svanvik J. The effect of lysophosphatidylcholine on gallbladder function in the cat. J Lab Clin Med. 1983 May;101(5):699–707. [PubMed] [Google Scholar]
  18. O'Sullivan M. G., Huggins E. M., Jr, Meade E. A., DeWitt D. L., McCall C. E. Lipopolysaccharide induces prostaglandin H synthase-2 in alveolar macrophages. Biochem Biophys Res Commun. 1992 Sep 16;187(2):1123–1127. doi: 10.1016/0006-291x(92)91313-f. [DOI] [PubMed] [Google Scholar]
  19. Ohno K., Fujimoto M., Hirata M. Protective effect of prostaglandin A2 against menadione-induced cell injury in cultured porcine aorta endothelial cells. Chem Biol Interact. 1991;78(1):67–75. doi: 10.1016/0009-2797(91)90103-e. [DOI] [PubMed] [Google Scholar]
  20. Snyder F. Platelet-activating factor and related acetylated lipids as potent biologically active cellular mediators. Am J Physiol. 1990 Nov;259(5 Pt 1):C697–C708. doi: 10.1152/ajpcell.1990.259.5.C697. [DOI] [PubMed] [Google Scholar]
  21. Taoka H. Experimental study on the pathogenesis of acute acalculous cholecystitis, with special reference to the roles of microcirculatory disturbances, free radicals and membrane-bound phospholipase A2. Gastroenterol Jpn. 1991 Oct;26(5):633–644. doi: 10.1007/BF02781681. [DOI] [PubMed] [Google Scholar]
  22. Vane J. R., Mitchell J. A., Appleton I., Tomlinson A., Bishop-Bailey D., Croxtall J., Willoughby D. A. Inducible isoforms of cyclooxygenase and nitric-oxide synthase in inflammation. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2046–2050. doi: 10.1073/pnas.91.6.2046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weltzien H. U. Cytolytic and membrane-perturbing properties of lysophosphatidylcholine. Biochim Biophys Acta. 1979 Aug 20;559(2-3):259–287. doi: 10.1016/0304-4157(79)90004-2. [DOI] [PubMed] [Google Scholar]
  24. Wong W. Y., DeWitt D. L., Smith W. L., Richards J. S. Rapid induction of prostaglandin endoperoxide synthase in rat preovulatory follicles by luteinizing hormone and cAMP is blocked by inhibitors of transcription and translation. Mol Endocrinol. 1989 Nov;3(11):1714–1723. doi: 10.1210/mend-3-11-1714. [DOI] [PubMed] [Google Scholar]

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