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. 1995 Jul;4(4):306–309. doi: 10.1155/S0962935195000494

Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi

S Delia 1, C M Mastroianni 1,✉, M Lichtner 1, F Mengoni 1, S Moretti 1, V Vullo 1
PMCID: PMC2365645  PMID: 18475656

Abstract

The production of interferon-γ and tumour necrosis factor-α was evaluated in the peripheral blood mononuclear cells (PBMCs) from healthy donors and AIDS patients after Rhodococcus equi infection in vitro. PBMCs from healthy donors secreted elevated levels of IFN-γ and TNF-α when challenged in vitro with killed R. equi, whereas the release of both cytokines was impaired in supernatant cultures from AIDS patients. We conclude that the failure of IFN-γ generation in AIDS patients in response to R. equi is not antigen-specific but it may reflect the global impairment of T-cell function. In such patients, however, the infection with R. equi, a facultative intracellular pathogen which survives and replicates within macrophages, may be responsible for the impairment in the TNF-α release, possibly enhancing the HIV-induced macrophage dysftmction.

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Selected References

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  1. Antoniades H. N., Bravo M. A., Avila R. E., Galanopoulos T., Neville-Golden J., Maxwell M., Selman M. Platelet-derived growth factor in idiopathic pulmonary fibrosis. J Clin Invest. 1990 Oct;86(4):1055–1064. doi: 10.1172/JCI114808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailly S., Ferrua B., Fay M., Gougerot-Pocidalo M. A. Differential regulation of IL 6, IL 1 A, IL 1 beta and TNF alpha production in LPS-stimulated human monocytes: role of cyclic AMP. Cytokine. 1990 May;2(3):205–210. doi: 10.1016/1043-4666(90)90017-n. [DOI] [PubMed] [Google Scholar]
  3. Bauman M. D., Jetten A. M., Bonner J. C., Kumar R. K., Bennett R. A., Brody A. R. Secretion of a platelet-derived growth factor homologue by rat alveolar macrophages exposed to particulates in vitro. Eur J Cell Biol. 1990 Apr;51(2):327–334. [PubMed] [Google Scholar]
  4. Bernard D. W., Rodriguez A., Rothblat G. H., Glick J. M. cAMP stimulates cholesteryl ester clearance to high density lipoproteins in J7774 macrophages. J Biol Chem. 1991 Jan 15;266(2):710–716. [PubMed] [Google Scholar]
  5. Bonney R. J., Burger S., Davies P., Kuehl F. A., Jr, Humes J. L. Prostaglandin E2 and prostacyclin elevate cyclic AMP levels in elicited populations of mouse peritoneal macrophages. Adv Prostaglandin Thromboxane Res. 1980;8:1691–1693. [PubMed] [Google Scholar]
  6. Brandwein S. R. Regulation of interleukin 1 production by mouse peritoneal macrophages. Effects of arachidonic acid metabolites, cyclic nucleotides, and interferons. J Biol Chem. 1986 Jul 5;261(19):8624–8632. [PubMed] [Google Scholar]
  7. Cassel D., Pfeuffer T. Mechanism of cholera toxin action: covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2669–2673. doi: 10.1073/pnas.75.6.2669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cox G. W., Mathieson B. J., Gandino L., Blasi E., Radzioch D., Varesio L. Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver. J Natl Cancer Inst. 1989 Oct 4;81(19):1492–1496. doi: 10.1093/jnci/81.19.1492. [DOI] [PubMed] [Google Scholar]
  9. Fabisiak J. P., Evans J. N., Kelley J. Increased expression of PDGF-B (c-sis) mRNA in rat lung precedes DNA synthesis and tissue repair during chronic hyperoxia. Am J Respir Cell Mol Biol. 1989 Sep;1(3):181–189. doi: 10.1165/ajrcmb/1.3.181. [DOI] [PubMed] [Google Scholar]
  10. Goldberg D. I., Khoo J. C. Stimulation of a neutral cholesteryl ester hydrolase by cAMP system in P388D1 macrophages. Biochim Biophys Acta. 1990 Jan 16;1042(1):132–137. doi: 10.1016/0005-2760(90)90067-8. [DOI] [PubMed] [Google Scholar]
  11. Halme J., White C., Kauma S., Estes J., Haskill S. Peritoneal macrophages from patients with endometriosis release growth factor activity in vitro. J Clin Endocrinol Metab. 1988 May;66(5):1044–1049. doi: 10.1210/jcem-66-5-1044. [DOI] [PubMed] [Google Scholar]
  12. Kassis S., Lee J. C., Hanna N. Effects of prostaglandins and cAMP levels on monocyte IL-1 production. Agents Actions. 1989 Jun;27(3-4):274–276. doi: 10.1007/BF01972795. [DOI] [PubMed] [Google Scholar]
  13. Kelley J. Cytokines of the lung. Am Rev Respir Dis. 1990 Mar;141(3):765–788. doi: 10.1164/ajrccm/141.3.765. [DOI] [PubMed] [Google Scholar]
  14. Kovacs E. J. Fibrogenic cytokines: the role of immune mediators in the development of scar tissue. Immunol Today. 1991 Jan;12(1):17–23. doi: 10.1016/0167-5699(91)90107-5. [DOI] [PubMed] [Google Scholar]
  15. Kovacs E. J., Kelley J. Intra-alveolar release of a competence-type growth factor after lung injury. Am Rev Respir Dis. 1986 Jan;133(1):68–72. doi: 10.1164/arrd.1986.133.1.68. [DOI] [PubMed] [Google Scholar]
  16. Kovacs E. J., Kelley J. Lymphokine regulation of macrophage-derived growth factor secretion following pulmonary injury. Am J Pathol. 1985 Nov;121(2):261–268. [PMC free article] [PubMed] [Google Scholar]
  17. Kovacs E. J., Oppenheim J. J., Young H. A. Induction of c-fos and c-myc expression in T lymphocytes after treatment with recombinant interleukin 1-alpha. J Immunol. 1986 Dec 1;137(11):3649–3651. [PubMed] [Google Scholar]
  18. Kumar R. K., Bennett R. A., Brody A. R. A homologue of platelet-derived growth factor produced by rat alveolar macrophages. FASEB J. 1988 Apr;2(7):2272–2277. doi: 10.1096/fasebj.2.7.3280379. [DOI] [PubMed] [Google Scholar]
  19. Leibovich S. J., Ross R. The role of the macrophage in wound repair. A study with hydrocortisone and antimacrophage serum. Am J Pathol. 1975 Jan;78(1):71–100. [PMC free article] [PubMed] [Google Scholar]
  20. Martinet Y., Bitterman P. B., Mornex J. F., Grotendorst G. R., Martin G. R., Crystal R. G. Activated human monocytes express the c-sis proto-oncogene and release a mediator showing PDGF-like activity. Nature. 1986 Jan 9;319(6049):158–160. doi: 10.1038/319158a0. [DOI] [PubMed] [Google Scholar]
  21. Martinet Y., Rom W. N., Grotendorst G. R., Martin G. R., Crystal R. G. Exaggerated spontaneous release of platelet-derived growth factor by alveolar macrophages from patients with idiopathic pulmonary fibrosis. N Engl J Med. 1987 Jul 23;317(4):202–209. doi: 10.1056/NEJM198707233170404. [DOI] [PubMed] [Google Scholar]
  22. Matsuoka J., Grotendorst G. R. Two peptides related to platelet-derived growth factor are present in human wound fluid. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4416–4420. doi: 10.1073/pnas.86.12.4416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mornex J. F., Martinet Y., Yamauchi K., Bitterman P. B., Grotendorst G. R., Chytil-Weir A., Martin G. R., Crystal R. G. Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages. J Clin Invest. 1986 Jul;78(1):61–66. doi: 10.1172/JCI112574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nagaoka I., Trapnell B. C., Crystal R. G. Upregulation of platelet-derived growth factor-A and -B gene expression in alveolar macrophages of individuals with idiopathic pulmonary fibrosis. J Clin Invest. 1990 Jun;85(6):2023–2027. doi: 10.1172/JCI114669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ross R., Masuda J., Raines E. W., Gown A. M., Katsuda S., Sasahara M., Malden L. T., Masuko H., Sato H. Localization of PDGF-B protein in macrophages in all phases of atherogenesis. Science. 1990 May 25;248(4958):1009–1012. doi: 10.1126/science.2343305. [DOI] [PubMed] [Google Scholar]
  26. Ross R. The pathogenesis of atherosclerosis--an update. N Engl J Med. 1986 Feb 20;314(8):488–500. doi: 10.1056/NEJM198602203140806. [DOI] [PubMed] [Google Scholar]
  27. Seamon K. B., Padgett W., Daly J. W. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3363–3367. doi: 10.1073/pnas.78.6.3363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Shimokado K., Raines E. W., Madtes D. K., Barrett T. B., Benditt E. P., Ross R. A significant part of macrophage-derived growth factor consists of at least two forms of PDGF. Cell. 1985 Nov;43(1):277–286. doi: 10.1016/0092-8674(85)90033-9. [DOI] [PubMed] [Google Scholar]
  29. Snyder L. S., Hertz M. I., Peterson M. S., Harmon K. R., Marinelli W. A., Henke C. A., Greenheck J. R., Chen B., Bitterman P. B. Acute lung injury. Pathogenesis of intraalveolar fibrosis. J Clin Invest. 1991 Aug;88(2):663–673. doi: 10.1172/JCI115351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stoller J. K. Systemic corticosteroids in stable chronic obstructive pulmonary disease. Do they work? Chest. 1987 Feb;91(2):155–156. doi: 10.1378/chest.91.2.155. [DOI] [PubMed] [Google Scholar]
  31. Sung S. S., Walters J. A. Increased cyclic AMP levels enhance IL-1 alpha and IL-1 beta mRNA expression and protein production in human myelomonocytic cell lines and monocytes. J Clin Invest. 1991 Dec;88(6):1915–1923. doi: 10.1172/JCI115515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Walsh M. J., LeLeiko N. S., Sterling K. M., Jr Regulation of types I, III, and IV procollagen mRNA synthesis in glucocorticoid-mediated intestinal development. J Biol Chem. 1987 Aug 5;262(22):10814–10818. [PubMed] [Google Scholar]
  33. Yamamoto H., Suzuki T. Prostaglandin-E2-induced activation of adenosine 3'-5' cyclic monophosphate-dependent protein kinases of a murine macrophage-like cell line (P388D1). J Immunol. 1987 Nov 15;139(10):3416–3421. [PubMed] [Google Scholar]
  34. Yamauchi K., Martinet Y., Basset P., Fells G. A., Crystal R. G. High levels of transforming growth factor-beta are present in the epithelial lining fluid of the normal human lower respiratory tract. Am Rev Respir Dis. 1988 Jun;137(6):1360–1363. doi: 10.1164/ajrccm/137.6.1360. [DOI] [PubMed] [Google Scholar]

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