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. 1996 Apr;5(2):110–112. doi: 10.1155/S096293519600018X

Opposite effects of interleukin-4 and interleukin-10 on nitric oxide production in murine macrophages

A Maru 1, S K Jackson 1,✉
PMCID: PMC2365784  PMID: 18475707

Abstract

Interleukin-4 (IL-4) and interleukin-10 (IL-10) were evaluated for their ability to inhibit the production of nitric oxide (NO) by interferon-γ (IFN-γ)- or lipopolysaccharide (LPS)-activated murine macrophages (RAW 264.7 and J774.2). Macrophages pre-treated with IL-4 and then stimulated with IFN-γ or LPS showed significant inhibition in their ability to produce NO as measured by nitrite production. Simultaneous treatment of IL-4 pre-incubated cells with IFN-γ and LPS together augmented nitrite accumulation. On the other hand, similar exposures of the macrophages to IL-10 followed by IFN-γ or LPS treatments resulted in significantly increased NO production. Thus IL-10 failed to suppress IFN-γ or LPS-induced NO production and showed opposite effects in these experiments to IL-4. We conclude that the two lymphokines have differing roles in the control of production of NO and might act to control the secretion of nitric oxide in vivo.

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Selected References

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