Skip to main content
NCBI home page
Mediators of Inflammation logoLink to Mediators of Inflammation
. 1996 Jun;5(3):210–217. doi: 10.1155/S0962935196000300

Expression of lipocortins in human bronchial epithelial cells: effects of IL-1β , TNF-α, LPS and dexamethasone

M M Verheggen 1,2, H I M de Bont 1, P W C Adriaansen-Soeting 1, B J A Goense 1, C J A M Tak 3, H C Hoogsteden 2, P Th W van Hal 1,2, M A Versnel 1,✉
PMCID: PMC2365795  PMID: 18475719

Abstract

In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1β, TNF-α or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE2 and 6-keto-PGF1α production was significantly increased. This IL-1β- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.

Full Text

The Full Text of this article is available as a PDF (1.4 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albright C. D., Grimley P. M., Jones R. T., Fontana J. A., Keenan K. P., Resau J. H. Cell-to-cell communication: a differential response to TGF-beta in normal and transformed (BEAS-2B) human bronchial epithelial cells. Carcinogenesis. 1991 Nov;12(11):1993–1999. doi: 10.1093/carcin/12.11.1993. [DOI] [PubMed] [Google Scholar]
  2. Ambrose M. P., Bahns C. L., Hunninghake G. W. Lipocortin I production by human alveolar macrophages. Am J Respir Cell Mol Biol. 1992 Jan;6(1):17–21. doi: 10.1165/ajrcmb/6.1.17. [DOI] [PubMed] [Google Scholar]
  3. Ambrose M. P., Hunninghake G. W. Corticosteroids increase lipocortin I in alveolar epithelial cells. Am J Respir Cell Mol Biol. 1990 Oct;3(4):349–353. doi: 10.1165/ajrcmb/3.4.349. [DOI] [PubMed] [Google Scholar]
  4. Benham F. J., Hodgkinson S., Davies K. E. A glyceraldehyde-3-phosphate dehydrogenase pseudogene on the short arm of the human X chromosomes defines a multigene family. EMBO J. 1984 Nov;3(11):2635–2640. doi: 10.1002/j.1460-2075.1984.tb02186.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  6. Croxtall J. D., Flower R. J. Lipocortin 1 mediates dexamethasone-induced growth arrest of the A549 lung adenocarcinoma cell line. Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3571–3575. doi: 10.1073/pnas.89.8.3571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. De Caterina R., Sicari R., Giannessi D., Paggiaro P. L., Paoletti P., Lazzerini G., Bernini W., Solito E., Parente L. Macrophage-specific eicosanoid synthesis inhibition and lipocortin-1 induction by glucocorticoids. J Appl Physiol (1985) 1993 Dec;75(6):2368–2375. doi: 10.1152/jappl.1993.75.6.2368. [DOI] [PubMed] [Google Scholar]
  8. Fava R. A., McKanna J., Cohen S. Lipocortin I (p35) is abundant in a restricted number of differentiated cell types in adult organs. J Cell Physiol. 1989 Nov;141(2):284–293. doi: 10.1002/jcp.1041410209. [DOI] [PubMed] [Google Scholar]
  9. Flower R. J., Rothwell N. J. Lipocortin-1: cellular mechanisms and clinical relevance. Trends Pharmacol Sci. 1994 Mar;15(3):71–76. doi: 10.1016/0165-6147(94)90281-x. [DOI] [PubMed] [Google Scholar]
  10. Goulding N. J., Guyre P. M. Regulation of inflammation by lipocortin 1. Immunol Today. 1992 Aug;13(8):295–297. doi: 10.1016/0167-5699(92)90040-E. [DOI] [PubMed] [Google Scholar]
  11. Jacquot J., Dupuit F., Elbtaouri H., Hinnrasky J., Antonicelli F., Haye B., Puchelle E. Production of lipocortin-like proteins by cultured human tracheal submucosal gland cells. FEBS Lett. 1990 Nov 12;274(1-2):131–135. doi: 10.1016/0014-5793(90)81347-q. [DOI] [PubMed] [Google Scholar]
  12. Koning H., Baert M. R., Groot R., Neijens H. J., Savelkoul H. F. Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR. Mediators Inflamm. 1995;4(3):196–204. doi: 10.1155/S0962935195000329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lechner J. F., Haugen A., McClendon I. A., Pettis E. W. Clonal growth of normal adult human bronchial epithelial cells in a serum-free medium. In Vitro. 1982 Jul;18(7):633–642. doi: 10.1007/BF02796396. [DOI] [PubMed] [Google Scholar]
  14. Pepinsky R. B., Sinclair L. K., Browning J. L., Mattaliano R. J., Smart J. E., Chow E. P., Falbel T., Ribolini A., Garwin J. L., Wallner B. P. Purification and partial sequence analysis of a 37-kDa protein that inhibits phospholipase A2 activity from rat peritoneal exudates. J Biol Chem. 1986 Mar 25;261(9):4239–4246. [PubMed] [Google Scholar]
  15. Pepinsky R. B., Tizard R., Mattaliano R. J., Sinclair L. K., Miller G. T., Browning J. L., Chow E. P., Burne C., Huang K. S., Pratt D. Five distinct calcium and phospholipid binding proteins share homology with lipocortin I. J Biol Chem. 1988 Aug 5;263(22):10799–10811. [PubMed] [Google Scholar]
  16. Piltch A., Sun L., Fava R. A., Hayashi J. Lipocortin-independent effect of dexamethasone on phospholipase activity in a thymic epithelial cell line. Biochem J. 1989 Jul 15;261(2):395–400. doi: 10.1042/bj2610395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reddel R. R., Ke Y., Gerwin B. I., McMenamin M. G., Lechner J. F., Su R. T., Brash D. E., Park J. B., Rhim J. S., Harris C. C. Transformation of human bronchial epithelial cells by infection with SV40 or adenovirus-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with a plasmid containing SV40 early region genes. Cancer Res. 1988 Apr 1;48(7):1904–1909. [PubMed] [Google Scholar]
  18. Smith T., Smith S. F., Buckingham J. C. A competitive enzyme-linked immunosorbent assay for the estimation of rat lipocortin 1. Biochem Soc Trans. 1990 Dec;18(6):1230–1231. doi: 10.1042/bst0181230. [DOI] [PubMed] [Google Scholar]
  19. Versnel M. A., Bouts M. J., Langerak A. W., van der Kwast T. H., Hoogsteden H. C., Hagemeijer A., Heldin C. H. Hydrocortisone-induced increase of PDGF beta-receptor expression in a human malignant mesothelioma cell line. Exp Cell Res. 1992 May;200(1):83–88. doi: 10.1016/s0014-4827(05)80074-6. [DOI] [PubMed] [Google Scholar]
  20. Vishwanatha J. K., Muns G., Beckmann J. D., Davis R. G., Rubinstein I. Differential expression of annexins I and II in bovine bronchial epithelial cells. Am J Respir Cell Mol Biol. 1995 Mar;12(3):280–286. doi: 10.1165/ajrcmb.12.3.7873194. [DOI] [PubMed] [Google Scholar]
  21. Wallner B. P., Mattaliano R. J., Hession C., Cate R. L., Tizard R., Sinclair L. K., Foeller C., Chow E. P., Browing J. L., Ramachandran K. L. Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature. 1986 Mar 6;320(6057):77–81. doi: 10.1038/320077a0. [DOI] [PubMed] [Google Scholar]
  22. Zijlstra F. J., Vincent J. E., Mol W. M., Hoogsteden H. C., Van Hal P. T., Jongejan R. C. Eicosanoid levels in bronchoalveolar lavage fluid of young female smokers and non-smokers. Eur J Clin Invest. 1992 May;22(5):301–306. doi: 10.1111/j.1365-2362.1992.tb01466.x. [DOI] [PubMed] [Google Scholar]

Articles from Mediators of Inflammation are provided here courtesy of Wiley

RESOURCES