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. 1996 Oct;5(5):324–327. doi: 10.1155/S0962935196000464

Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells

J Padrón 1,✉, L Glaría 2, O Martinez 2, M Torres 2, E Lopez 2, R Delgado 2, L Caveda 2, A Rojas 2
PMCID: PMC2365802  PMID: 18475725

Abstract

The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, 3H-thymidine uptake of CTLL-2 cells in response to different concentrations of interleukin 2. Withdrawal of L-arginine from culture medium resulted as potent as the higher inhibition obtained when blocking nitric oxide synthase with L-arginine analogues. Furthermore, intermedial concentrations of Larginine and exogenous nitric oxide donors were found for achieving optimal IL2-induced proliferation of CTLL-2. These findings prompted us to suggest that intra- and/or inter-cellular nitric oxide signalling may contribute to the modulation of the IL2 mitogenic effect upon cytotoxic T lymphocytes.

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Selected References

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