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. 1994 Mar;68(3):1293–1300. doi: 10.1128/jvi.68.3.1293-1300.1994

Identification of Evi-3, a novel common site of retroviral integration in mouse AKXD B-cell lymphomas.

M J Justice 1, H C Morse 3rd 1, N A Jenkins 1, N G Copeland 1
PMCID: PMC236582  PMID: 8107195

Abstract

We have identified a novel common site of ecotropic viral integration called ecotropic viral integration site 3 (Evi-3) in B-cell lineage lymphomas of the AKXD recombinant inbred strains of mice. A number of virally induced pre-B-, B-, myeloid, and T-cell lymphomas were screened for viral rearrangements at Evi-3; rearrangements were found in pre-B- and B-cell lymphomas but not in other hematopoietic tumors. Genetic mapping studies localized Evi-3 to mouse chromosome 18, distinct from proto-oncogene and common viral integration loci identified previously in the mouse. Each proviral integration at Evi-3 is contained within a 200-bp region that lies inside a CpG island. All but one of the proviruses have integrated in the same 5'-to-3' transcriptional orientation. Transcripts from Evi-3 are expressed in a developmentally regulated manner in B cells. Taken together, these data suggest that Evi-3 represents a novel proto-oncogene involved in mouse B-cell lymphomas.

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Selected References

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