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. 1996 Dec;5(6):443–447. doi: 10.1155/S0962935196000610

Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes

J M Huebner 1,✉, R R Eversole 1, W F Jackson 1, C D Mackenzie 2, S R Stapleton 1, L J Beuving 1
PMCID: PMC2365822  PMID: 18475750

Abstract

The mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration-dependent release of leukotriene (LT) C4 when exposed to the Ca2+ ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5-lipoxygenase activating protein antagonist MK-886 (0.1 μM), nominally Ca2+ and Mg2+-free incubation media and by activation of protein kinase C via phorbol 12-myristate 13-acetate (50 nM). These findings establish a model system for investigating LTC4 profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC4 biosynthesis is phosphoregulated.

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Selected References

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